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MiR-186-3p attenuates tumorigenesis of cervical cancer by targeting IGF1

BACKGROUND: Mounting evidence in the cancer literature suggests that microRNAs (miRNAs) influence the progression of human cancer cells by targeting protein-coding genes. How insulin-like growth factor 1(IGF1) and miR-186-3p contribute to the development of cervical cancer (CC) remains unclear. This...

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Autores principales: Lu, Xiurong, Song, Xiao, Hao, Xiaohui, Liu, Xiaoyu, Zhang, Xianyu, Yuan, Na, Ma, Huan, Zhang, Zhilin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276452/
https://www.ncbi.nlm.nih.gov/pubmed/34253194
http://dx.doi.org/10.1186/s12957-021-02317-z
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author Lu, Xiurong
Song, Xiao
Hao, Xiaohui
Liu, Xiaoyu
Zhang, Xianyu
Yuan, Na
Ma, Huan
Zhang, Zhilin
author_facet Lu, Xiurong
Song, Xiao
Hao, Xiaohui
Liu, Xiaoyu
Zhang, Xianyu
Yuan, Na
Ma, Huan
Zhang, Zhilin
author_sort Lu, Xiurong
collection PubMed
description BACKGROUND: Mounting evidence in the cancer literature suggests that microRNAs (miRNAs) influence the progression of human cancer cells by targeting protein-coding genes. How insulin-like growth factor 1(IGF1) and miR-186-3p contribute to the development of cervical cancer (CC) remains unclear. This study examined the regulatory roles of miR-186-3p and IGF1 in CC development. METHODS: Gene expression levels were determined by qRT-PCR. Proliferation, migration, and apoptosis of CC and normal cells were determined by MTT, Transwell, and caspase-3 activity assays, respectively. Dual-luciferase reporter activity and RNA pull-down assays were performed to identify the target gene of miR-186-3p. RESULTS: IGF1 was the target of miR-186-3p. The expression of miR-186-3p inhibited cell proliferation and migration abilities of CC cell lines, but induced the apoptosis rate of CC cells. IGF1 could restore the inhibitory effects of miR-186-3p on the proliferation, migration, and apoptosis abilities of CC cells. Experimental results revealed that miR-186-3p could inhibit IGF1 expression, thereby reducing the viability of CC cells. CONCLUSIONS: The data suggest that targeting of IGF1 by miR-186-3p could be crucial in regulating the progression of CC.
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spelling pubmed-82764522021-07-13 MiR-186-3p attenuates tumorigenesis of cervical cancer by targeting IGF1 Lu, Xiurong Song, Xiao Hao, Xiaohui Liu, Xiaoyu Zhang, Xianyu Yuan, Na Ma, Huan Zhang, Zhilin World J Surg Oncol Research BACKGROUND: Mounting evidence in the cancer literature suggests that microRNAs (miRNAs) influence the progression of human cancer cells by targeting protein-coding genes. How insulin-like growth factor 1(IGF1) and miR-186-3p contribute to the development of cervical cancer (CC) remains unclear. This study examined the regulatory roles of miR-186-3p and IGF1 in CC development. METHODS: Gene expression levels were determined by qRT-PCR. Proliferation, migration, and apoptosis of CC and normal cells were determined by MTT, Transwell, and caspase-3 activity assays, respectively. Dual-luciferase reporter activity and RNA pull-down assays were performed to identify the target gene of miR-186-3p. RESULTS: IGF1 was the target of miR-186-3p. The expression of miR-186-3p inhibited cell proliferation and migration abilities of CC cell lines, but induced the apoptosis rate of CC cells. IGF1 could restore the inhibitory effects of miR-186-3p on the proliferation, migration, and apoptosis abilities of CC cells. Experimental results revealed that miR-186-3p could inhibit IGF1 expression, thereby reducing the viability of CC cells. CONCLUSIONS: The data suggest that targeting of IGF1 by miR-186-3p could be crucial in regulating the progression of CC. BioMed Central 2021-07-12 /pmc/articles/PMC8276452/ /pubmed/34253194 http://dx.doi.org/10.1186/s12957-021-02317-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Lu, Xiurong
Song, Xiao
Hao, Xiaohui
Liu, Xiaoyu
Zhang, Xianyu
Yuan, Na
Ma, Huan
Zhang, Zhilin
MiR-186-3p attenuates tumorigenesis of cervical cancer by targeting IGF1
title MiR-186-3p attenuates tumorigenesis of cervical cancer by targeting IGF1
title_full MiR-186-3p attenuates tumorigenesis of cervical cancer by targeting IGF1
title_fullStr MiR-186-3p attenuates tumorigenesis of cervical cancer by targeting IGF1
title_full_unstemmed MiR-186-3p attenuates tumorigenesis of cervical cancer by targeting IGF1
title_short MiR-186-3p attenuates tumorigenesis of cervical cancer by targeting IGF1
title_sort mir-186-3p attenuates tumorigenesis of cervical cancer by targeting igf1
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276452/
https://www.ncbi.nlm.nih.gov/pubmed/34253194
http://dx.doi.org/10.1186/s12957-021-02317-z
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