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Comparative Transcriptome and DNA Methylation Analysis of Phenotypic Plasticity in the Pacific Abalone (Haliotis discus hannai)

Phenotypic plasticity is an adaptive mechanism used by organisms to cope with environmental fluctuations. Pacific abalone (Haliotis discus hannai) are large-scale farmed in the temperate area of northern China and in the warmer waters of southern China. RNA-seq and comparative transcriptomic analysi...

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Autores principales: Huang, Zekun, Xiao, Qizhen, Yu, Feng, Gan, Yang, Lu, Chengkuan, Peng, Wenzhu, Zhang, Yifang, Luo, Xuan, Chen, Nan, You, Weiwei, Ke, Caihuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277243/
https://www.ncbi.nlm.nih.gov/pubmed/34267674
http://dx.doi.org/10.3389/fphys.2021.683499
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author Huang, Zekun
Xiao, Qizhen
Yu, Feng
Gan, Yang
Lu, Chengkuan
Peng, Wenzhu
Zhang, Yifang
Luo, Xuan
Chen, Nan
You, Weiwei
Ke, Caihuan
author_facet Huang, Zekun
Xiao, Qizhen
Yu, Feng
Gan, Yang
Lu, Chengkuan
Peng, Wenzhu
Zhang, Yifang
Luo, Xuan
Chen, Nan
You, Weiwei
Ke, Caihuan
author_sort Huang, Zekun
collection PubMed
description Phenotypic plasticity is an adaptive mechanism used by organisms to cope with environmental fluctuations. Pacific abalone (Haliotis discus hannai) are large-scale farmed in the temperate area of northern China and in the warmer waters of southern China. RNA-seq and comparative transcriptomic analysis here were performed to determine if the northern and southern populations have evolved divergent plasticity and if functional differences are associated with protein synthesis and growth-related biological progress. The DNA methylation (5mC) landscape of H. discus hannai from the two populations using whole genomic bisulfite sequencing (WGBS), exhibited different epigenetic patterns. The southern population had significant genomic hypo-methylation that may have resulted from long-term acclimation to heat stress. Combining 790 differentially expressed genes (DEGs) and 7635 differentially methylated genes (DMGs), we found that methylation within the gene body might be important in predicting abalone gene expression. Genes related to growth, development, transduction, and apoptosis may be regulated by methylation and could explain the phenotypic divergence of H. discus hannai. Our findings not only emphasize the significant roles of adaptive plasticity in the acclimation of H. discus hannai to high temperatures but also provide a new understanding of the epigenetic mechanism underlying the phenotypic plasticity in adaptation to climate change for marine organisms.
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spelling pubmed-82772432021-07-14 Comparative Transcriptome and DNA Methylation Analysis of Phenotypic Plasticity in the Pacific Abalone (Haliotis discus hannai) Huang, Zekun Xiao, Qizhen Yu, Feng Gan, Yang Lu, Chengkuan Peng, Wenzhu Zhang, Yifang Luo, Xuan Chen, Nan You, Weiwei Ke, Caihuan Front Physiol Physiology Phenotypic plasticity is an adaptive mechanism used by organisms to cope with environmental fluctuations. Pacific abalone (Haliotis discus hannai) are large-scale farmed in the temperate area of northern China and in the warmer waters of southern China. RNA-seq and comparative transcriptomic analysis here were performed to determine if the northern and southern populations have evolved divergent plasticity and if functional differences are associated with protein synthesis and growth-related biological progress. The DNA methylation (5mC) landscape of H. discus hannai from the two populations using whole genomic bisulfite sequencing (WGBS), exhibited different epigenetic patterns. The southern population had significant genomic hypo-methylation that may have resulted from long-term acclimation to heat stress. Combining 790 differentially expressed genes (DEGs) and 7635 differentially methylated genes (DMGs), we found that methylation within the gene body might be important in predicting abalone gene expression. Genes related to growth, development, transduction, and apoptosis may be regulated by methylation and could explain the phenotypic divergence of H. discus hannai. Our findings not only emphasize the significant roles of adaptive plasticity in the acclimation of H. discus hannai to high temperatures but also provide a new understanding of the epigenetic mechanism underlying the phenotypic plasticity in adaptation to climate change for marine organisms. Frontiers Media S.A. 2021-06-29 /pmc/articles/PMC8277243/ /pubmed/34267674 http://dx.doi.org/10.3389/fphys.2021.683499 Text en Copyright © 2021 Huang, Xiao, Yu, Gan, Lu, Peng, Zhang, Luo, Chen, You and Ke. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Huang, Zekun
Xiao, Qizhen
Yu, Feng
Gan, Yang
Lu, Chengkuan
Peng, Wenzhu
Zhang, Yifang
Luo, Xuan
Chen, Nan
You, Weiwei
Ke, Caihuan
Comparative Transcriptome and DNA Methylation Analysis of Phenotypic Plasticity in the Pacific Abalone (Haliotis discus hannai)
title Comparative Transcriptome and DNA Methylation Analysis of Phenotypic Plasticity in the Pacific Abalone (Haliotis discus hannai)
title_full Comparative Transcriptome and DNA Methylation Analysis of Phenotypic Plasticity in the Pacific Abalone (Haliotis discus hannai)
title_fullStr Comparative Transcriptome and DNA Methylation Analysis of Phenotypic Plasticity in the Pacific Abalone (Haliotis discus hannai)
title_full_unstemmed Comparative Transcriptome and DNA Methylation Analysis of Phenotypic Plasticity in the Pacific Abalone (Haliotis discus hannai)
title_short Comparative Transcriptome and DNA Methylation Analysis of Phenotypic Plasticity in the Pacific Abalone (Haliotis discus hannai)
title_sort comparative transcriptome and dna methylation analysis of phenotypic plasticity in the pacific abalone (haliotis discus hannai)
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277243/
https://www.ncbi.nlm.nih.gov/pubmed/34267674
http://dx.doi.org/10.3389/fphys.2021.683499
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