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Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs

KEY MESSAGE: Rose has 19 MLO genes. Of these, RhMLO1 and RhMLO2 were shown to be required for powdery mildew infection, which suggests their potential as susceptibility targets towards disease resistance. ABSTRACT: Powdery mildew, caused by Podosphaera pannosa, is one of the most serious and widespr...

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Autores principales: Fang, Peihong, Arens, Paul, Liu, Xintong, Zhang, Xin, Lakwani, Deepika, Foucher, Fabrice, Clotault, Jérémy, Geike, Juliane, Kaufmann, Helgard, Debener, Thomas, Bai, Yuling, Zhang, Zhao, Smulders, Marinus J. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277636/
https://www.ncbi.nlm.nih.gov/pubmed/33934211
http://dx.doi.org/10.1007/s00122-021-03838-7
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author Fang, Peihong
Arens, Paul
Liu, Xintong
Zhang, Xin
Lakwani, Deepika
Foucher, Fabrice
Clotault, Jérémy
Geike, Juliane
Kaufmann, Helgard
Debener, Thomas
Bai, Yuling
Zhang, Zhao
Smulders, Marinus J. M.
author_facet Fang, Peihong
Arens, Paul
Liu, Xintong
Zhang, Xin
Lakwani, Deepika
Foucher, Fabrice
Clotault, Jérémy
Geike, Juliane
Kaufmann, Helgard
Debener, Thomas
Bai, Yuling
Zhang, Zhao
Smulders, Marinus J. M.
author_sort Fang, Peihong
collection PubMed
description KEY MESSAGE: Rose has 19 MLO genes. Of these, RhMLO1 and RhMLO2 were shown to be required for powdery mildew infection, which suggests their potential as susceptibility targets towards disease resistance. ABSTRACT: Powdery mildew, caused by Podosphaera pannosa, is one of the most serious and widespread fungal diseases for roses, especially in greenhouse-grown cut roses. It has been shown that certain MLO genes are involved in powdery mildew susceptibility and that loss of function in these genes in various crops leads to broad-spectrum, long-lasting resistance against this fungal disease. For this reason, these MLO genes are called susceptibility genes. We carried out a genome-wide identification of the MLO gene family in the Rosa chinensis genome, and screened for allelic variants among 22 accessions from seven different Rosa species using re-sequencing and transcriptome data. We identified 19 MLO genes in rose, of which four are candidate genes for functional homologs in clade V, which is the clade containing all dicot MLO susceptibility genes. We detected a total of 198 different allelic variants in the set of Rosa species and accessions, corresponding to 5–15 different alleles for each of the genes. Some diploid Rosa species shared alleles with tetraploid rose cultivars, consistent with the notion that diploid species have contributed to the formation of tetraploid roses. Among the four RhMLO genes in clade V, we demonstrated using expression study, virus-induced gene silencing as well as transient RNAi silencing that two of them, RhMLO1 and RhMLO2, are required for infection by P. pannosa and suggest their potential as susceptibility targets for powdery mildew resistance breeding in rose. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00122-021-03838-7.
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spelling pubmed-82776362021-07-20 Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs Fang, Peihong Arens, Paul Liu, Xintong Zhang, Xin Lakwani, Deepika Foucher, Fabrice Clotault, Jérémy Geike, Juliane Kaufmann, Helgard Debener, Thomas Bai, Yuling Zhang, Zhao Smulders, Marinus J. M. Theor Appl Genet Original Article KEY MESSAGE: Rose has 19 MLO genes. Of these, RhMLO1 and RhMLO2 were shown to be required for powdery mildew infection, which suggests their potential as susceptibility targets towards disease resistance. ABSTRACT: Powdery mildew, caused by Podosphaera pannosa, is one of the most serious and widespread fungal diseases for roses, especially in greenhouse-grown cut roses. It has been shown that certain MLO genes are involved in powdery mildew susceptibility and that loss of function in these genes in various crops leads to broad-spectrum, long-lasting resistance against this fungal disease. For this reason, these MLO genes are called susceptibility genes. We carried out a genome-wide identification of the MLO gene family in the Rosa chinensis genome, and screened for allelic variants among 22 accessions from seven different Rosa species using re-sequencing and transcriptome data. We identified 19 MLO genes in rose, of which four are candidate genes for functional homologs in clade V, which is the clade containing all dicot MLO susceptibility genes. We detected a total of 198 different allelic variants in the set of Rosa species and accessions, corresponding to 5–15 different alleles for each of the genes. Some diploid Rosa species shared alleles with tetraploid rose cultivars, consistent with the notion that diploid species have contributed to the formation of tetraploid roses. Among the four RhMLO genes in clade V, we demonstrated using expression study, virus-induced gene silencing as well as transient RNAi silencing that two of them, RhMLO1 and RhMLO2, are required for infection by P. pannosa and suggest their potential as susceptibility targets for powdery mildew resistance breeding in rose. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00122-021-03838-7. Springer Berlin Heidelberg 2021-05-02 2021 /pmc/articles/PMC8277636/ /pubmed/33934211 http://dx.doi.org/10.1007/s00122-021-03838-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Fang, Peihong
Arens, Paul
Liu, Xintong
Zhang, Xin
Lakwani, Deepika
Foucher, Fabrice
Clotault, Jérémy
Geike, Juliane
Kaufmann, Helgard
Debener, Thomas
Bai, Yuling
Zhang, Zhao
Smulders, Marinus J. M.
Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs
title Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs
title_full Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs
title_fullStr Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs
title_full_unstemmed Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs
title_short Analysis of allelic variants of RhMLO genes in rose and functional studies on susceptibility to powdery mildew related to clade V homologs
title_sort analysis of allelic variants of rhmlo genes in rose and functional studies on susceptibility to powdery mildew related to clade v homologs
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277636/
https://www.ncbi.nlm.nih.gov/pubmed/33934211
http://dx.doi.org/10.1007/s00122-021-03838-7
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