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Toxocara canis Infection Alters lncRNA and mRNA Expression Profiles of Dog Bone Marrow

Bone marrow is the main hematopoietic organ that produces red blood cells, granulocytes, monocyte/macrophages, megakaryocytes, lymphocytes, and myeloid dendritic cells. Many of these cells play roles in the pathogenesis of Toxocara canis infection, and understanding how infection alters the dynamics...

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Autores principales: Zheng, Wen-Bin, Zou, Yang, Liu, Qing, Hu, Min-Hua, Elsheikha, Hany M., Zhu, Xing-Quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277978/
https://www.ncbi.nlm.nih.gov/pubmed/34277631
http://dx.doi.org/10.3389/fcell.2021.688128
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author Zheng, Wen-Bin
Zou, Yang
Liu, Qing
Hu, Min-Hua
Elsheikha, Hany M.
Zhu, Xing-Quan
author_facet Zheng, Wen-Bin
Zou, Yang
Liu, Qing
Hu, Min-Hua
Elsheikha, Hany M.
Zhu, Xing-Quan
author_sort Zheng, Wen-Bin
collection PubMed
description Bone marrow is the main hematopoietic organ that produces red blood cells, granulocytes, monocyte/macrophages, megakaryocytes, lymphocytes, and myeloid dendritic cells. Many of these cells play roles in the pathogenesis of Toxocara canis infection, and understanding how infection alters the dynamics of transcription regulation in bone marrow is therefore critical for deciphering the global changes in the dog transcriptional signatures during T. canis infection. In this study, long non-coding RNA (lncRNA) and messenger RNA (mRNA) expression profiles in the bone marrow of Beagle dogs infected with T. canis were determined at 12 h post-infection (hpi), 24 hpi, 96 hpi, and 36 days post-infection (dpi). RNA-sequencing and bioinformatics analysis identified 1,098, 984, 1,120, and 1,305 differentially expressed lncRNAs (DElncRNAs), and 196, 253, 223, and 328 differentially expressed mRNAs (DEmRNAs) at 12 h, 24 h, 96 h, and 36 days after infection, respectively. We also identified 29, 36, 38, and 68 DEmRNAs potentially cis-regulated by 44, 44, 51, and 80 DElncRNAs at 12 hpi, 24 hpi, 96 hpi, and 36 dpi, respectively. To validate the sequencing findings, qRT-PCR was performed on 10 randomly selected transcripts. Many altered genes were involved in the differentiation of bone marrow cells. GO of DElncRNAs and GO and KEGG pathway analyses of DEmRNAs revealed alterations in several signaling pathways, including pathways involved in energy metabolism, amino acid biosynthesis and metabolism, Wnt signaling pathway, Huntington's disease, HIF-1 signaling pathway, cGMP–PKG signaling pathway, dilated cardiomyopathy, and adrenergic signaling in cardiomyocytes. These findings revealed that bone marrow of T. canis-infected dogs exhibits distinct lncRNA and mRNA expression patterns compared to healthy control dogs. Our data provide novel insights into T. canis interaction with the definitive host and shed light on the significance of the non-coding portion of the dog genome in the pathogenesis of toxocariasis.
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spelling pubmed-82779782021-07-15 Toxocara canis Infection Alters lncRNA and mRNA Expression Profiles of Dog Bone Marrow Zheng, Wen-Bin Zou, Yang Liu, Qing Hu, Min-Hua Elsheikha, Hany M. Zhu, Xing-Quan Front Cell Dev Biol Cell and Developmental Biology Bone marrow is the main hematopoietic organ that produces red blood cells, granulocytes, monocyte/macrophages, megakaryocytes, lymphocytes, and myeloid dendritic cells. Many of these cells play roles in the pathogenesis of Toxocara canis infection, and understanding how infection alters the dynamics of transcription regulation in bone marrow is therefore critical for deciphering the global changes in the dog transcriptional signatures during T. canis infection. In this study, long non-coding RNA (lncRNA) and messenger RNA (mRNA) expression profiles in the bone marrow of Beagle dogs infected with T. canis were determined at 12 h post-infection (hpi), 24 hpi, 96 hpi, and 36 days post-infection (dpi). RNA-sequencing and bioinformatics analysis identified 1,098, 984, 1,120, and 1,305 differentially expressed lncRNAs (DElncRNAs), and 196, 253, 223, and 328 differentially expressed mRNAs (DEmRNAs) at 12 h, 24 h, 96 h, and 36 days after infection, respectively. We also identified 29, 36, 38, and 68 DEmRNAs potentially cis-regulated by 44, 44, 51, and 80 DElncRNAs at 12 hpi, 24 hpi, 96 hpi, and 36 dpi, respectively. To validate the sequencing findings, qRT-PCR was performed on 10 randomly selected transcripts. Many altered genes were involved in the differentiation of bone marrow cells. GO of DElncRNAs and GO and KEGG pathway analyses of DEmRNAs revealed alterations in several signaling pathways, including pathways involved in energy metabolism, amino acid biosynthesis and metabolism, Wnt signaling pathway, Huntington's disease, HIF-1 signaling pathway, cGMP–PKG signaling pathway, dilated cardiomyopathy, and adrenergic signaling in cardiomyocytes. These findings revealed that bone marrow of T. canis-infected dogs exhibits distinct lncRNA and mRNA expression patterns compared to healthy control dogs. Our data provide novel insights into T. canis interaction with the definitive host and shed light on the significance of the non-coding portion of the dog genome in the pathogenesis of toxocariasis. Frontiers Media S.A. 2021-06-30 /pmc/articles/PMC8277978/ /pubmed/34277631 http://dx.doi.org/10.3389/fcell.2021.688128 Text en Copyright © 2021 Zheng, Zou, Liu, Hu, Elsheikha and Zhu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Zheng, Wen-Bin
Zou, Yang
Liu, Qing
Hu, Min-Hua
Elsheikha, Hany M.
Zhu, Xing-Quan
Toxocara canis Infection Alters lncRNA and mRNA Expression Profiles of Dog Bone Marrow
title Toxocara canis Infection Alters lncRNA and mRNA Expression Profiles of Dog Bone Marrow
title_full Toxocara canis Infection Alters lncRNA and mRNA Expression Profiles of Dog Bone Marrow
title_fullStr Toxocara canis Infection Alters lncRNA and mRNA Expression Profiles of Dog Bone Marrow
title_full_unstemmed Toxocara canis Infection Alters lncRNA and mRNA Expression Profiles of Dog Bone Marrow
title_short Toxocara canis Infection Alters lncRNA and mRNA Expression Profiles of Dog Bone Marrow
title_sort toxocara canis infection alters lncrna and mrna expression profiles of dog bone marrow
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277978/
https://www.ncbi.nlm.nih.gov/pubmed/34277631
http://dx.doi.org/10.3389/fcell.2021.688128
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