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Bone marrow stromal cells (BMSCs CD45(‐)/CD44(+)/CD73(+)/CD90(+)) isolated from osteoporotic mice SAM/P6 as a novel model for osteoporosis investigation

Available therapies aimed at treating age‐related osteoporosis are still insufficient. Therefore, designing reliable in vitro model for the analysis of molecular mechanisms underlying senile osteoporosis is highly required. We have isolated and characterized progenitor cells isolated from bone marro...

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Autores principales: Sikora, Mateusz, Śmieszek, Agnieszka, Marycz, Krzysztof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278098/
https://www.ncbi.nlm.nih.gov/pubmed/34075722
http://dx.doi.org/10.1111/jcmm.16667
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author Sikora, Mateusz
Śmieszek, Agnieszka
Marycz, Krzysztof
author_facet Sikora, Mateusz
Śmieszek, Agnieszka
Marycz, Krzysztof
author_sort Sikora, Mateusz
collection PubMed
description Available therapies aimed at treating age‐related osteoporosis are still insufficient. Therefore, designing reliable in vitro model for the analysis of molecular mechanisms underlying senile osteoporosis is highly required. We have isolated and characterized progenitor cells isolated from bone marrow (BMSCs) of osteoporotic mice strain SAM/P6 (BMSC(SAM/P6)). The cytophysiology of BMSC(SAM/P6) was for the first time compared with BMSCs isolated from healthy BALB/c mice (BMSC(BALB/c)). Characterization of the cells included evaluation of their multipotency, morphology and determination of specific phenotype. Viability of BMSCs cultures was determined in reference to apoptosis profile, metabolic activity, oxidative stress, mitochondrial membrane potential and caspase activation. Additionally, expression of relevant biomarkers was determined with RT‐qPCR. Obtained results indicated that BMSC(SAM/P6) and BMSC(BALB/c) show the typical phenotype of mesenchymal stromal cells (CD44+, CD73+, CD90+) and do not express CD45. Further, BMSC(SAM/P6) were characterized by deteriorated multipotency, decreased metabolic activity and increased apoptosis occurrence, accompanied by elevated oxidative stress and mitochondria depolarisation. The transcriptome analyses showed that BMSC(SAM/P6) are distinguished by lowered expression of molecules crucial for proper osteogenesis, including Coll‐1, Opg and Opn. However, the expression of Trap, DANCR1 and miR‐124‐3p was significantly up‐regulated. Obtained results show that BMSC(SAM/P6) present features of progenitor cells with disturbed metabolism and could serve as appropriate model for in vitro investigation of age‐dependent osteoporosis.
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spelling pubmed-82780982021-07-15 Bone marrow stromal cells (BMSCs CD45(‐)/CD44(+)/CD73(+)/CD90(+)) isolated from osteoporotic mice SAM/P6 as a novel model for osteoporosis investigation Sikora, Mateusz Śmieszek, Agnieszka Marycz, Krzysztof J Cell Mol Med Original Articles Available therapies aimed at treating age‐related osteoporosis are still insufficient. Therefore, designing reliable in vitro model for the analysis of molecular mechanisms underlying senile osteoporosis is highly required. We have isolated and characterized progenitor cells isolated from bone marrow (BMSCs) of osteoporotic mice strain SAM/P6 (BMSC(SAM/P6)). The cytophysiology of BMSC(SAM/P6) was for the first time compared with BMSCs isolated from healthy BALB/c mice (BMSC(BALB/c)). Characterization of the cells included evaluation of their multipotency, morphology and determination of specific phenotype. Viability of BMSCs cultures was determined in reference to apoptosis profile, metabolic activity, oxidative stress, mitochondrial membrane potential and caspase activation. Additionally, expression of relevant biomarkers was determined with RT‐qPCR. Obtained results indicated that BMSC(SAM/P6) and BMSC(BALB/c) show the typical phenotype of mesenchymal stromal cells (CD44+, CD73+, CD90+) and do not express CD45. Further, BMSC(SAM/P6) were characterized by deteriorated multipotency, decreased metabolic activity and increased apoptosis occurrence, accompanied by elevated oxidative stress and mitochondria depolarisation. The transcriptome analyses showed that BMSC(SAM/P6) are distinguished by lowered expression of molecules crucial for proper osteogenesis, including Coll‐1, Opg and Opn. However, the expression of Trap, DANCR1 and miR‐124‐3p was significantly up‐regulated. Obtained results show that BMSC(SAM/P6) present features of progenitor cells with disturbed metabolism and could serve as appropriate model for in vitro investigation of age‐dependent osteoporosis. John Wiley and Sons Inc. 2021-06-01 2021-07 /pmc/articles/PMC8278098/ /pubmed/34075722 http://dx.doi.org/10.1111/jcmm.16667 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Sikora, Mateusz
Śmieszek, Agnieszka
Marycz, Krzysztof
Bone marrow stromal cells (BMSCs CD45(‐)/CD44(+)/CD73(+)/CD90(+)) isolated from osteoporotic mice SAM/P6 as a novel model for osteoporosis investigation
title Bone marrow stromal cells (BMSCs CD45(‐)/CD44(+)/CD73(+)/CD90(+)) isolated from osteoporotic mice SAM/P6 as a novel model for osteoporosis investigation
title_full Bone marrow stromal cells (BMSCs CD45(‐)/CD44(+)/CD73(+)/CD90(+)) isolated from osteoporotic mice SAM/P6 as a novel model for osteoporosis investigation
title_fullStr Bone marrow stromal cells (BMSCs CD45(‐)/CD44(+)/CD73(+)/CD90(+)) isolated from osteoporotic mice SAM/P6 as a novel model for osteoporosis investigation
title_full_unstemmed Bone marrow stromal cells (BMSCs CD45(‐)/CD44(+)/CD73(+)/CD90(+)) isolated from osteoporotic mice SAM/P6 as a novel model for osteoporosis investigation
title_short Bone marrow stromal cells (BMSCs CD45(‐)/CD44(+)/CD73(+)/CD90(+)) isolated from osteoporotic mice SAM/P6 as a novel model for osteoporosis investigation
title_sort bone marrow stromal cells (bmscs cd45(‐)/cd44(+)/cd73(+)/cd90(+)) isolated from osteoporotic mice sam/p6 as a novel model for osteoporosis investigation
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278098/
https://www.ncbi.nlm.nih.gov/pubmed/34075722
http://dx.doi.org/10.1111/jcmm.16667
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