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Genetic mapping and phenotypic analysis of shot(H.3.2) in Drosophila melanogaster
Genetic screens are used to identify genes involved in specific biological processes. An EMS mutagenesis screen in Drosophila melanogaster identified growth control phenotypes in the developing eye. One mutant line from this screen, H.3.2, was phenotypically characterized using the FLP/FRT system an...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Caltech Library
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278230/ https://www.ncbi.nlm.nih.gov/pubmed/34278244 http://dx.doi.org/10.17912/micropub.biology.000418 |
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| author | Talley, Elyse M Watts, Charlie T Aboyer, Sonia Adamson, Madeline G Akoto, Harriet AB Altemus, Haley Avella, Philip J Bailey, Rebecca Bell, Elizabeth R Bell, Katheryn L Breneman, Kelsey Burkhart, Jessica S Chanley, Logan J Cook, Savannah S DesLaurier, Mackenzie T Dorsey, Timothy R Doyle, Cassandra J Egloff, Merris E Fasawe, Ayoola S Garcia, Katy K Graves, Nathaniel P Gray, Tyler K Gustafson, Evan M Hall, Makayla J Hayes, Jaden D Holic, Lindsay J Jarvis, Brice A Klos, Piotr S Kritzmire, Sidney Kuzovko, Lera Lainez, Edwyna McCoy, Shamerra Mierendorf, James C Neri, Nicole A Neville, Caley R Osborn, Kelley Parker, Kaitlyn Parks, Megan E Peck, Kylee Pitt, Robyn Platta, Matthew E Powell, Brianna Rodriguez, Katalina Ruiz, Clara Schaefer, Mariah N Shields, Amanda B Smiley, Jasmine B Stauffer, Briona Straub, Devan Sweeney, John L Termine, Kaitlyn M Thomas, Brett Toth, Sophia D Veile, Taylor R Walker, Kayla S Webster, Paige N Woodard, Brian J Yoder, Quentin L Young, McKenzie K Zeedyk, McKenzie L Ziegler, Logan N Bieser, Kayla L Puthoff, David P Stamm, Joyce Vrailas-Mortimer, Alysia D Kagey, Jacob D Merkle, Julie A |
| author_facet | Talley, Elyse M Watts, Charlie T Aboyer, Sonia Adamson, Madeline G Akoto, Harriet AB Altemus, Haley Avella, Philip J Bailey, Rebecca Bell, Elizabeth R Bell, Katheryn L Breneman, Kelsey Burkhart, Jessica S Chanley, Logan J Cook, Savannah S DesLaurier, Mackenzie T Dorsey, Timothy R Doyle, Cassandra J Egloff, Merris E Fasawe, Ayoola S Garcia, Katy K Graves, Nathaniel P Gray, Tyler K Gustafson, Evan M Hall, Makayla J Hayes, Jaden D Holic, Lindsay J Jarvis, Brice A Klos, Piotr S Kritzmire, Sidney Kuzovko, Lera Lainez, Edwyna McCoy, Shamerra Mierendorf, James C Neri, Nicole A Neville, Caley R Osborn, Kelley Parker, Kaitlyn Parks, Megan E Peck, Kylee Pitt, Robyn Platta, Matthew E Powell, Brianna Rodriguez, Katalina Ruiz, Clara Schaefer, Mariah N Shields, Amanda B Smiley, Jasmine B Stauffer, Briona Straub, Devan Sweeney, John L Termine, Kaitlyn M Thomas, Brett Toth, Sophia D Veile, Taylor R Walker, Kayla S Webster, Paige N Woodard, Brian J Yoder, Quentin L Young, McKenzie K Zeedyk, McKenzie L Ziegler, Logan N Bieser, Kayla L Puthoff, David P Stamm, Joyce Vrailas-Mortimer, Alysia D Kagey, Jacob D Merkle, Julie A |
| author_sort | Talley, Elyse M |
| collection | PubMed |
| description | Genetic screens are used to identify genes involved in specific biological processes. An EMS mutagenesis screen in Drosophila melanogaster identified growth control phenotypes in the developing eye. One mutant line from this screen, H.3.2, was phenotypically characterized using the FLP/FRT system and genetically mapped by complementation analysis and genomic sequencing by undergraduate students participating in the multi-institution Fly-CURE consortium. H.3.2 was found to have a nonsense mutation in short stop (shot), anortholog of the mammalian spectraplakin dystonin (DST). shot and DST are involved in cytoskeletal organization and play roles during cell growth and proliferation. |
| format | Online Article Text |
| id | pubmed-8278230 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | Caltech Library |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-82782302021-07-15 Genetic mapping and phenotypic analysis of shot(H.3.2) in Drosophila melanogaster Talley, Elyse M Watts, Charlie T Aboyer, Sonia Adamson, Madeline G Akoto, Harriet AB Altemus, Haley Avella, Philip J Bailey, Rebecca Bell, Elizabeth R Bell, Katheryn L Breneman, Kelsey Burkhart, Jessica S Chanley, Logan J Cook, Savannah S DesLaurier, Mackenzie T Dorsey, Timothy R Doyle, Cassandra J Egloff, Merris E Fasawe, Ayoola S Garcia, Katy K Graves, Nathaniel P Gray, Tyler K Gustafson, Evan M Hall, Makayla J Hayes, Jaden D Holic, Lindsay J Jarvis, Brice A Klos, Piotr S Kritzmire, Sidney Kuzovko, Lera Lainez, Edwyna McCoy, Shamerra Mierendorf, James C Neri, Nicole A Neville, Caley R Osborn, Kelley Parker, Kaitlyn Parks, Megan E Peck, Kylee Pitt, Robyn Platta, Matthew E Powell, Brianna Rodriguez, Katalina Ruiz, Clara Schaefer, Mariah N Shields, Amanda B Smiley, Jasmine B Stauffer, Briona Straub, Devan Sweeney, John L Termine, Kaitlyn M Thomas, Brett Toth, Sophia D Veile, Taylor R Walker, Kayla S Webster, Paige N Woodard, Brian J Yoder, Quentin L Young, McKenzie K Zeedyk, McKenzie L Ziegler, Logan N Bieser, Kayla L Puthoff, David P Stamm, Joyce Vrailas-Mortimer, Alysia D Kagey, Jacob D Merkle, Julie A MicroPubl Biol New Finding Genetic screens are used to identify genes involved in specific biological processes. An EMS mutagenesis screen in Drosophila melanogaster identified growth control phenotypes in the developing eye. One mutant line from this screen, H.3.2, was phenotypically characterized using the FLP/FRT system and genetically mapped by complementation analysis and genomic sequencing by undergraduate students participating in the multi-institution Fly-CURE consortium. H.3.2 was found to have a nonsense mutation in short stop (shot), anortholog of the mammalian spectraplakin dystonin (DST). shot and DST are involved in cytoskeletal organization and play roles during cell growth and proliferation. Caltech Library 2021-07-13 /pmc/articles/PMC8278230/ /pubmed/34278244 http://dx.doi.org/10.17912/micropub.biology.000418 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| spellingShingle | New Finding Talley, Elyse M Watts, Charlie T Aboyer, Sonia Adamson, Madeline G Akoto, Harriet AB Altemus, Haley Avella, Philip J Bailey, Rebecca Bell, Elizabeth R Bell, Katheryn L Breneman, Kelsey Burkhart, Jessica S Chanley, Logan J Cook, Savannah S DesLaurier, Mackenzie T Dorsey, Timothy R Doyle, Cassandra J Egloff, Merris E Fasawe, Ayoola S Garcia, Katy K Graves, Nathaniel P Gray, Tyler K Gustafson, Evan M Hall, Makayla J Hayes, Jaden D Holic, Lindsay J Jarvis, Brice A Klos, Piotr S Kritzmire, Sidney Kuzovko, Lera Lainez, Edwyna McCoy, Shamerra Mierendorf, James C Neri, Nicole A Neville, Caley R Osborn, Kelley Parker, Kaitlyn Parks, Megan E Peck, Kylee Pitt, Robyn Platta, Matthew E Powell, Brianna Rodriguez, Katalina Ruiz, Clara Schaefer, Mariah N Shields, Amanda B Smiley, Jasmine B Stauffer, Briona Straub, Devan Sweeney, John L Termine, Kaitlyn M Thomas, Brett Toth, Sophia D Veile, Taylor R Walker, Kayla S Webster, Paige N Woodard, Brian J Yoder, Quentin L Young, McKenzie K Zeedyk, McKenzie L Ziegler, Logan N Bieser, Kayla L Puthoff, David P Stamm, Joyce Vrailas-Mortimer, Alysia D Kagey, Jacob D Merkle, Julie A Genetic mapping and phenotypic analysis of shot(H.3.2) in Drosophila melanogaster |
| title | Genetic mapping and phenotypic analysis of shot(H.3.2) in Drosophila melanogaster |
| title_full | Genetic mapping and phenotypic analysis of shot(H.3.2) in Drosophila melanogaster |
| title_fullStr | Genetic mapping and phenotypic analysis of shot(H.3.2) in Drosophila melanogaster |
| title_full_unstemmed | Genetic mapping and phenotypic analysis of shot(H.3.2) in Drosophila melanogaster |
| title_short | Genetic mapping and phenotypic analysis of shot(H.3.2) in Drosophila melanogaster |
| title_sort | genetic mapping and phenotypic analysis of shot(h.3.2) in drosophila melanogaster |
| topic | New Finding |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278230/ https://www.ncbi.nlm.nih.gov/pubmed/34278244 http://dx.doi.org/10.17912/micropub.biology.000418 |
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