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Chondroitin Sulfate Disaccharides in the Gas Phase: Differentiation and Conformational Constraints
[Image: see text] Glycosaminoglycans (GAGs) are a family of complex carbohydrates vital to all mammalian organisms and involved in numerous biological processes. Chondroitin and dermatan sulfate, an important class of GAGs, are linear macromolecules consisting of disaccharide building blocks of N-ac...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279649/ https://www.ncbi.nlm.nih.gov/pubmed/33979516 http://dx.doi.org/10.1021/acs.jpca.1c02463 |
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author | Lettow, Maike Greis, Kim Grabarics, Márkó Horlebein, Jan Miller, Rebecca L. Meijer, Gerard von Helden, Gert Pagel, Kevin |
author_facet | Lettow, Maike Greis, Kim Grabarics, Márkó Horlebein, Jan Miller, Rebecca L. Meijer, Gerard von Helden, Gert Pagel, Kevin |
author_sort | Lettow, Maike |
collection | PubMed |
description | [Image: see text] Glycosaminoglycans (GAGs) are a family of complex carbohydrates vital to all mammalian organisms and involved in numerous biological processes. Chondroitin and dermatan sulfate, an important class of GAGs, are linear macromolecules consisting of disaccharide building blocks of N-acetylgalactosamine and two different uronic acids. The varying degree and the site of sulfation render their characterization challenging. Here, we combine mass spectrometry with cryogenic infrared spectroscopy in the wavenumber range from 1000 to 1800 cm(–1). Fingerprint spectra were recorded for a comprehensive set of disaccharides bearing all known motifs of sulfation. In addition, state-of-the-art quantum chemical calculations were performed to aid the understanding of the differences in the experimental fingerprint spectra. The results show that the degree and position of charged sulfate groups define the size of the conformational landscape in the gas phase. The detailed understanding of cryogenic infrared spectroscopy for acidic and often highly sulfated glycans may pave the way to utilize the technique in fragment-based sequencing approaches. |
format | Online Article Text |
id | pubmed-8279649 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-82796492021-07-15 Chondroitin Sulfate Disaccharides in the Gas Phase: Differentiation and Conformational Constraints Lettow, Maike Greis, Kim Grabarics, Márkó Horlebein, Jan Miller, Rebecca L. Meijer, Gerard von Helden, Gert Pagel, Kevin J Phys Chem A [Image: see text] Glycosaminoglycans (GAGs) are a family of complex carbohydrates vital to all mammalian organisms and involved in numerous biological processes. Chondroitin and dermatan sulfate, an important class of GAGs, are linear macromolecules consisting of disaccharide building blocks of N-acetylgalactosamine and two different uronic acids. The varying degree and the site of sulfation render their characterization challenging. Here, we combine mass spectrometry with cryogenic infrared spectroscopy in the wavenumber range from 1000 to 1800 cm(–1). Fingerprint spectra were recorded for a comprehensive set of disaccharides bearing all known motifs of sulfation. In addition, state-of-the-art quantum chemical calculations were performed to aid the understanding of the differences in the experimental fingerprint spectra. The results show that the degree and position of charged sulfate groups define the size of the conformational landscape in the gas phase. The detailed understanding of cryogenic infrared spectroscopy for acidic and often highly sulfated glycans may pave the way to utilize the technique in fragment-based sequencing approaches. American Chemical Society 2021-05-12 2021-05-27 /pmc/articles/PMC8279649/ /pubmed/33979516 http://dx.doi.org/10.1021/acs.jpca.1c02463 Text en © 2021 The Authors. Published by American Chemical Society Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Lettow, Maike Greis, Kim Grabarics, Márkó Horlebein, Jan Miller, Rebecca L. Meijer, Gerard von Helden, Gert Pagel, Kevin Chondroitin Sulfate Disaccharides in the Gas Phase: Differentiation and Conformational Constraints |
title | Chondroitin Sulfate Disaccharides in the Gas Phase:
Differentiation and Conformational Constraints |
title_full | Chondroitin Sulfate Disaccharides in the Gas Phase:
Differentiation and Conformational Constraints |
title_fullStr | Chondroitin Sulfate Disaccharides in the Gas Phase:
Differentiation and Conformational Constraints |
title_full_unstemmed | Chondroitin Sulfate Disaccharides in the Gas Phase:
Differentiation and Conformational Constraints |
title_short | Chondroitin Sulfate Disaccharides in the Gas Phase:
Differentiation and Conformational Constraints |
title_sort | chondroitin sulfate disaccharides in the gas phase:
differentiation and conformational constraints |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279649/ https://www.ncbi.nlm.nih.gov/pubmed/33979516 http://dx.doi.org/10.1021/acs.jpca.1c02463 |
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