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Neuroprotective effect of minocycline on rat retinal ischemia-reperfusion injury
PURPOSE: To examine the neuroprotective effect of minocycline on retinal ischemia-reperfusion (IR) injury in rats and investigate its possible mechanism of action. METHODS: Retinal IR injury was established by increasing the intraocular pressure in rats up to 110 mmHg for 60 min. The animals with re...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Vision
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279698/ https://www.ncbi.nlm.nih.gov/pubmed/34295142 |
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author | Li, Xiaoli Ye, Zhiqiang Pei, Shuaili Zheng, Dongliang Zhu, Lin |
author_facet | Li, Xiaoli Ye, Zhiqiang Pei, Shuaili Zheng, Dongliang Zhu, Lin |
author_sort | Li, Xiaoli |
collection | PubMed |
description | PURPOSE: To examine the neuroprotective effect of minocycline on retinal ischemia-reperfusion (IR) injury in rats and investigate its possible mechanism of action. METHODS: Retinal IR injury was established by increasing the intraocular pressure in rats up to 110 mmHg for 60 min. The animals with retinal IR injury were intraperitoneally injected with 22.5 mg/kg minocycline twice a day for 14 days. The control group received the same amount of saline. Subsequently, funduscopic examination, retinal thickness measurement, retinal microvascular morphology, full-field electroretinography (ERG), retinal apoptotic cell count, and remaining retinal ganglion cell (RGC) count were performed. The expression of iNOS, Bax, Bcl2, IL-1α, IL-6, TNF-α, caspase-3, GFAP, Iba-1, Hif-1α, and Nrf2 was examined with real-time PCR and western blotting. RESULTS: Minocycline treatment prevented IR-induced rat retinal edema and retinal cells apoptosis at the early stage and alleviated retina atrophy, blood vessel tortuosity, functional photoreceptor damage, and RGC degeneration at the late stage of the IR injury. At the molecular level, minocycline affected retinal gene and protein expression induced by IR. CONCLUSIONS: The results suggested that minocycline has a neuroprotective effect on rat retinal IR injury, possibly through anti-inflammation, antiapoptosis, antioxidation, and inhibition of microglial activation. |
format | Online Article Text |
id | pubmed-8279698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-82796982021-07-21 Neuroprotective effect of minocycline on rat retinal ischemia-reperfusion injury Li, Xiaoli Ye, Zhiqiang Pei, Shuaili Zheng, Dongliang Zhu, Lin Mol Vis Research Article PURPOSE: To examine the neuroprotective effect of minocycline on retinal ischemia-reperfusion (IR) injury in rats and investigate its possible mechanism of action. METHODS: Retinal IR injury was established by increasing the intraocular pressure in rats up to 110 mmHg for 60 min. The animals with retinal IR injury were intraperitoneally injected with 22.5 mg/kg minocycline twice a day for 14 days. The control group received the same amount of saline. Subsequently, funduscopic examination, retinal thickness measurement, retinal microvascular morphology, full-field electroretinography (ERG), retinal apoptotic cell count, and remaining retinal ganglion cell (RGC) count were performed. The expression of iNOS, Bax, Bcl2, IL-1α, IL-6, TNF-α, caspase-3, GFAP, Iba-1, Hif-1α, and Nrf2 was examined with real-time PCR and western blotting. RESULTS: Minocycline treatment prevented IR-induced rat retinal edema and retinal cells apoptosis at the early stage and alleviated retina atrophy, blood vessel tortuosity, functional photoreceptor damage, and RGC degeneration at the late stage of the IR injury. At the molecular level, minocycline affected retinal gene and protein expression induced by IR. CONCLUSIONS: The results suggested that minocycline has a neuroprotective effect on rat retinal IR injury, possibly through anti-inflammation, antiapoptosis, antioxidation, and inhibition of microglial activation. Molecular Vision 2021-07-08 /pmc/articles/PMC8279698/ /pubmed/34295142 Text en Copyright © 2021 Molecular Vision. https://creativecommons.org/licenses/by-nc-nd/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed. |
spellingShingle | Research Article Li, Xiaoli Ye, Zhiqiang Pei, Shuaili Zheng, Dongliang Zhu, Lin Neuroprotective effect of minocycline on rat retinal ischemia-reperfusion injury |
title | Neuroprotective effect of minocycline on rat retinal ischemia-reperfusion injury |
title_full | Neuroprotective effect of minocycline on rat retinal ischemia-reperfusion injury |
title_fullStr | Neuroprotective effect of minocycline on rat retinal ischemia-reperfusion injury |
title_full_unstemmed | Neuroprotective effect of minocycline on rat retinal ischemia-reperfusion injury |
title_short | Neuroprotective effect of minocycline on rat retinal ischemia-reperfusion injury |
title_sort | neuroprotective effect of minocycline on rat retinal ischemia-reperfusion injury |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279698/ https://www.ncbi.nlm.nih.gov/pubmed/34295142 |
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