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SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis

SARS-CoV-2 is the etiologic agent of coronavirus disease 2019 (COVID-19) and is mainly detected by RT-PCR methods from upper respiratory specimens, as recommended by the World Health Organization. Oro/nasopharyngeal swabbing can be discomfortable to the patients, requires trained healthcare personne...

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Autores principales: de Oliveira, Cristina Mendes, Brochi, Leila, Scarpelli, Luciano Cesar, Lopes, Annelise Correa Wengerkievicz, Levi, José Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279922/
https://www.ncbi.nlm.nih.gov/pubmed/34273438
http://dx.doi.org/10.1016/j.jviromet.2021.114241
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author de Oliveira, Cristina Mendes
Brochi, Leila
Scarpelli, Luciano Cesar
Lopes, Annelise Correa Wengerkievicz
Levi, José Eduardo
author_facet de Oliveira, Cristina Mendes
Brochi, Leila
Scarpelli, Luciano Cesar
Lopes, Annelise Correa Wengerkievicz
Levi, José Eduardo
author_sort de Oliveira, Cristina Mendes
collection PubMed
description SARS-CoV-2 is the etiologic agent of coronavirus disease 2019 (COVID-19) and is mainly detected by RT-PCR methods from upper respiratory specimens, as recommended by the World Health Organization. Oro/nasopharyngeal swabbing can be discomfortable to the patients, requires trained healthcare personnel and may generate aerosol, increasing the risk of nosocomial infections. In this study, we describe two SARS-CoV-2 RNA extraction-free single RT-PCR protocols on saliva samples and compared the results with the paired oro/nasopharyngeal swab specimens from 400 patients. The two saliva protocols demonstrated a substantial agreement when compared to the oro/nasopharyngeal swab protocol. Moreover, the positivity rate of saliva protocols increased according to the disease period. The 95 % limit of detection of one of the therefore implemented saliva protocol was determined as 9441 copies/mL. Our results support the conclusion that RNA extraction-free RT-PCR using self-collected saliva specimens is an alternative to nasopharyngeal swabs, especially in the early phase of symptom onset.
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spelling pubmed-82799222021-07-20 SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis de Oliveira, Cristina Mendes Brochi, Leila Scarpelli, Luciano Cesar Lopes, Annelise Correa Wengerkievicz Levi, José Eduardo J Virol Methods Article SARS-CoV-2 is the etiologic agent of coronavirus disease 2019 (COVID-19) and is mainly detected by RT-PCR methods from upper respiratory specimens, as recommended by the World Health Organization. Oro/nasopharyngeal swabbing can be discomfortable to the patients, requires trained healthcare personnel and may generate aerosol, increasing the risk of nosocomial infections. In this study, we describe two SARS-CoV-2 RNA extraction-free single RT-PCR protocols on saliva samples and compared the results with the paired oro/nasopharyngeal swab specimens from 400 patients. The two saliva protocols demonstrated a substantial agreement when compared to the oro/nasopharyngeal swab protocol. Moreover, the positivity rate of saliva protocols increased according to the disease period. The 95 % limit of detection of one of the therefore implemented saliva protocol was determined as 9441 copies/mL. Our results support the conclusion that RNA extraction-free RT-PCR using self-collected saliva specimens is an alternative to nasopharyngeal swabs, especially in the early phase of symptom onset. Elsevier B.V. 2021-10 2021-07-15 /pmc/articles/PMC8279922/ /pubmed/34273438 http://dx.doi.org/10.1016/j.jviromet.2021.114241 Text en © 2021 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
de Oliveira, Cristina Mendes
Brochi, Leila
Scarpelli, Luciano Cesar
Lopes, Annelise Correa Wengerkievicz
Levi, José Eduardo
SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis
title SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis
title_full SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis
title_fullStr SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis
title_full_unstemmed SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis
title_short SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis
title_sort sars-cov-2 saliva testing is a useful tool for covid-19 diagnosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279922/
https://www.ncbi.nlm.nih.gov/pubmed/34273438
http://dx.doi.org/10.1016/j.jviromet.2021.114241
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