Testing individual and pooled saliva samples for sars-cov-2 nucleic acid: a prospective study

Control of the rapid spread of the SARS-CoV-2 virus requires efficient testing. We collected paired nasopharyngeal swab (NPs) and saliva samples from 303 subjects (52.8% symptomatic) at a drive-through testing center; 18% of whom tested positive. The NPs, salivas and five saliva pools were tested fo...

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Autores principales: Migueres, Marion, Vellas, Camille, Abravanel, Florence, Da Silva, Isabelle, Dimeglio, Chloé, Ferrer, Venicia, Raymond, Stéphanie, Mansuy, Jean-Michel, Izopet, Jacques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279932/
https://www.ncbi.nlm.nih.gov/pubmed/34364098
http://dx.doi.org/10.1016/j.diagmicrobio.2021.115478
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author Migueres, Marion
Vellas, Camille
Abravanel, Florence
Da Silva, Isabelle
Dimeglio, Chloé
Ferrer, Venicia
Raymond, Stéphanie
Mansuy, Jean-Michel
Izopet, Jacques
author_facet Migueres, Marion
Vellas, Camille
Abravanel, Florence
Da Silva, Isabelle
Dimeglio, Chloé
Ferrer, Venicia
Raymond, Stéphanie
Mansuy, Jean-Michel
Izopet, Jacques
author_sort Migueres, Marion
collection PubMed
description Control of the rapid spread of the SARS-CoV-2 virus requires efficient testing. We collected paired nasopharyngeal swab (NPs) and saliva samples from 303 subjects (52.8% symptomatic) at a drive-through testing center; 18% of whom tested positive. The NPs, salivas and five saliva pools were tested for SARS-CoV-2 RNA using the Aptima™ assay and a laboratory-developed test (LDT) on the Panther-Fusion™ Hologic® platform. The saliva sensitivity was 80% (LDT) and 87.5% (Aptima™) whereas that of NPs was 96.4% in both assays. The pooled saliva sensitivity of 72.7% (LDT) and 75% (Aptima™) was not significantly different of that of individual saliva testing. Saliva specimens appear to be suitable for sensitive non-invasive assays to detect SARS-CoV-2 nucleic acid; pooling them for a single test will improve laboratory throughput.
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spelling pubmed-82799322021-07-20 Testing individual and pooled saliva samples for sars-cov-2 nucleic acid: a prospective study Migueres, Marion Vellas, Camille Abravanel, Florence Da Silva, Isabelle Dimeglio, Chloé Ferrer, Venicia Raymond, Stéphanie Mansuy, Jean-Michel Izopet, Jacques Diagn Microbiol Infect Dis Article Control of the rapid spread of the SARS-CoV-2 virus requires efficient testing. We collected paired nasopharyngeal swab (NPs) and saliva samples from 303 subjects (52.8% symptomatic) at a drive-through testing center; 18% of whom tested positive. The NPs, salivas and five saliva pools were tested for SARS-CoV-2 RNA using the Aptima™ assay and a laboratory-developed test (LDT) on the Panther-Fusion™ Hologic® platform. The saliva sensitivity was 80% (LDT) and 87.5% (Aptima™) whereas that of NPs was 96.4% in both assays. The pooled saliva sensitivity of 72.7% (LDT) and 75% (Aptima™) was not significantly different of that of individual saliva testing. Saliva specimens appear to be suitable for sensitive non-invasive assays to detect SARS-CoV-2 nucleic acid; pooling them for a single test will improve laboratory throughput. Elsevier Inc. 2021-11 2021-07-15 /pmc/articles/PMC8279932/ /pubmed/34364098 http://dx.doi.org/10.1016/j.diagmicrobio.2021.115478 Text en © 2021 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Migueres, Marion
Vellas, Camille
Abravanel, Florence
Da Silva, Isabelle
Dimeglio, Chloé
Ferrer, Venicia
Raymond, Stéphanie
Mansuy, Jean-Michel
Izopet, Jacques
Testing individual and pooled saliva samples for sars-cov-2 nucleic acid: a prospective study
title Testing individual and pooled saliva samples for sars-cov-2 nucleic acid: a prospective study
title_full Testing individual and pooled saliva samples for sars-cov-2 nucleic acid: a prospective study
title_fullStr Testing individual and pooled saliva samples for sars-cov-2 nucleic acid: a prospective study
title_full_unstemmed Testing individual and pooled saliva samples for sars-cov-2 nucleic acid: a prospective study
title_short Testing individual and pooled saliva samples for sars-cov-2 nucleic acid: a prospective study
title_sort testing individual and pooled saliva samples for sars-cov-2 nucleic acid: a prospective study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279932/
https://www.ncbi.nlm.nih.gov/pubmed/34364098
http://dx.doi.org/10.1016/j.diagmicrobio.2021.115478
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