Immunologic mechanisms of seasonal influenza vaccination administered by microneedle patch from a randomized phase I trial

In a phase 1 randomized, single-center clinical trial, inactivated influenza virus vaccine delivered through dissolvable microneedle patches (MNPs) was found to be safe and immunogenic. Here, we compare the humoral and cellular immunologic responses in a subset of participants receiving influenza va...

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Autores principales: Rouphael, Nadine G., Lai, Lilin, Tandon, Sonia, McCullough, Michele Paine, Kong, Yunchuan, Kabbani, Sarah, Natrajan, Muktha S., Xu, Yongxian, Zhu, Yerun, Wang, Dongli, O’Shea, Jesse, Sherman, Amy, Yu, Tianwei, Henry, Sebastien, McAllister, Devin, Stadlbauer, Daniel, Khurana, Surender, Golding, Hana, Krammer, Florian, Mulligan, Mark J., Prausnitz, Mark R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280206/
https://www.ncbi.nlm.nih.gov/pubmed/34262052
http://dx.doi.org/10.1038/s41541-021-00353-0
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author Rouphael, Nadine G.
Lai, Lilin
Tandon, Sonia
McCullough, Michele Paine
Kong, Yunchuan
Kabbani, Sarah
Natrajan, Muktha S.
Xu, Yongxian
Zhu, Yerun
Wang, Dongli
O’Shea, Jesse
Sherman, Amy
Yu, Tianwei
Henry, Sebastien
McAllister, Devin
Stadlbauer, Daniel
Khurana, Surender
Golding, Hana
Krammer, Florian
Mulligan, Mark J.
Prausnitz, Mark R.
author_facet Rouphael, Nadine G.
Lai, Lilin
Tandon, Sonia
McCullough, Michele Paine
Kong, Yunchuan
Kabbani, Sarah
Natrajan, Muktha S.
Xu, Yongxian
Zhu, Yerun
Wang, Dongli
O’Shea, Jesse
Sherman, Amy
Yu, Tianwei
Henry, Sebastien
McAllister, Devin
Stadlbauer, Daniel
Khurana, Surender
Golding, Hana
Krammer, Florian
Mulligan, Mark J.
Prausnitz, Mark R.
author_sort Rouphael, Nadine G.
collection PubMed
description In a phase 1 randomized, single-center clinical trial, inactivated influenza virus vaccine delivered through dissolvable microneedle patches (MNPs) was found to be safe and immunogenic. Here, we compare the humoral and cellular immunologic responses in a subset of participants receiving influenza vaccination by MNP to the intramuscular (IM) route of administration. We collected serum, plasma, and peripheral blood mononuclear cells in 22 participants up to 180 days post-vaccination. Hemagglutination inhibition (HAI) titers and antibody avidity were similar after MNP and IM vaccination, even though MNP vaccination used a lower antigen dose. MNPs generated higher neuraminidase inhibition (NAI) titers for all three influenza virus vaccine strains tested and triggered a larger percentage of circulating T follicular helper cells (CD4 + CXCR5 + CXCR3 + ICOS + PD-1+) compared to the IM route. Our study indicates that inactivated influenza virus vaccination by MNP produces humoral and cellular immune response that are similar or greater than IM vaccination.
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spelling pubmed-82802062021-07-23 Immunologic mechanisms of seasonal influenza vaccination administered by microneedle patch from a randomized phase I trial Rouphael, Nadine G. Lai, Lilin Tandon, Sonia McCullough, Michele Paine Kong, Yunchuan Kabbani, Sarah Natrajan, Muktha S. Xu, Yongxian Zhu, Yerun Wang, Dongli O’Shea, Jesse Sherman, Amy Yu, Tianwei Henry, Sebastien McAllister, Devin Stadlbauer, Daniel Khurana, Surender Golding, Hana Krammer, Florian Mulligan, Mark J. Prausnitz, Mark R. NPJ Vaccines Article In a phase 1 randomized, single-center clinical trial, inactivated influenza virus vaccine delivered through dissolvable microneedle patches (MNPs) was found to be safe and immunogenic. Here, we compare the humoral and cellular immunologic responses in a subset of participants receiving influenza vaccination by MNP to the intramuscular (IM) route of administration. We collected serum, plasma, and peripheral blood mononuclear cells in 22 participants up to 180 days post-vaccination. Hemagglutination inhibition (HAI) titers and antibody avidity were similar after MNP and IM vaccination, even though MNP vaccination used a lower antigen dose. MNPs generated higher neuraminidase inhibition (NAI) titers for all three influenza virus vaccine strains tested and triggered a larger percentage of circulating T follicular helper cells (CD4 + CXCR5 + CXCR3 + ICOS + PD-1+) compared to the IM route. Our study indicates that inactivated influenza virus vaccination by MNP produces humoral and cellular immune response that are similar or greater than IM vaccination. Nature Publishing Group UK 2021-07-14 /pmc/articles/PMC8280206/ /pubmed/34262052 http://dx.doi.org/10.1038/s41541-021-00353-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Rouphael, Nadine G.
Lai, Lilin
Tandon, Sonia
McCullough, Michele Paine
Kong, Yunchuan
Kabbani, Sarah
Natrajan, Muktha S.
Xu, Yongxian
Zhu, Yerun
Wang, Dongli
O’Shea, Jesse
Sherman, Amy
Yu, Tianwei
Henry, Sebastien
McAllister, Devin
Stadlbauer, Daniel
Khurana, Surender
Golding, Hana
Krammer, Florian
Mulligan, Mark J.
Prausnitz, Mark R.
Immunologic mechanisms of seasonal influenza vaccination administered by microneedle patch from a randomized phase I trial
title Immunologic mechanisms of seasonal influenza vaccination administered by microneedle patch from a randomized phase I trial
title_full Immunologic mechanisms of seasonal influenza vaccination administered by microneedle patch from a randomized phase I trial
title_fullStr Immunologic mechanisms of seasonal influenza vaccination administered by microneedle patch from a randomized phase I trial
title_full_unstemmed Immunologic mechanisms of seasonal influenza vaccination administered by microneedle patch from a randomized phase I trial
title_short Immunologic mechanisms of seasonal influenza vaccination administered by microneedle patch from a randomized phase I trial
title_sort immunologic mechanisms of seasonal influenza vaccination administered by microneedle patch from a randomized phase i trial
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280206/
https://www.ncbi.nlm.nih.gov/pubmed/34262052
http://dx.doi.org/10.1038/s41541-021-00353-0
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