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Intrauterine growth restriction weakens anticontractile influence of NO in coronary arteries of adult rats

Intrauterine growth restriction (IUGR) is one of the most common pathologies of pregnancy. The cardiovascular consequences of IUGR do not disappear in adulthood and can manifest themselves in pathological alterations of vasomotor control. The hypothesis was tested that IUGR weakens anticontractile i...

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Autores principales: Selivanova, Ekaterina K., Shvetsova, Anastasia A., Shilova, Lyubov D., Tarasova, Olga S., Gaynullina, Dina K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280217/
https://www.ncbi.nlm.nih.gov/pubmed/34262070
http://dx.doi.org/10.1038/s41598-021-93491-3
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author Selivanova, Ekaterina K.
Shvetsova, Anastasia A.
Shilova, Lyubov D.
Tarasova, Olga S.
Gaynullina, Dina K.
author_facet Selivanova, Ekaterina K.
Shvetsova, Anastasia A.
Shilova, Lyubov D.
Tarasova, Olga S.
Gaynullina, Dina K.
author_sort Selivanova, Ekaterina K.
collection PubMed
description Intrauterine growth restriction (IUGR) is one of the most common pathologies of pregnancy. The cardiovascular consequences of IUGR do not disappear in adulthood and can manifest themselves in pathological alterations of vasomotor control. The hypothesis was tested that IUGR weakens anticontractile influence of NO and augments procontractile influence of Rho-kinase in arteries of adult offspring. To model IUGR in the rat, dams were 50% food restricted starting from the gestational day 11 till delivery. Mesenteric and coronary arteries of male offspring were studied at the age of 3 months using wire myography, qPCR, and Western blotting. Contractile responses of mesenteric arteries to α(1)-adrenoceptor agonist methoxamine as well as influences of NO and Rho-kinase did not differ between control and IUGR rats. However, coronary arteries of IUGR rats demonstrated elevated contraction to thromboxane A2 receptor agonist U46619 due to weakened anticontractile influence of NO and enhanced role of Rho-kinase in the endothelium. This was accompanied by reduced abundance of SODI protein and elevated content of RhoA protein in coronary arteries of IUGR rats. IUGR considerably changes the regulation of coronary vascular tone in adulthood and, therefore, can serve as a risk factor for the development of cardiac disorders.
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spelling pubmed-82802172021-07-15 Intrauterine growth restriction weakens anticontractile influence of NO in coronary arteries of adult rats Selivanova, Ekaterina K. Shvetsova, Anastasia A. Shilova, Lyubov D. Tarasova, Olga S. Gaynullina, Dina K. Sci Rep Article Intrauterine growth restriction (IUGR) is one of the most common pathologies of pregnancy. The cardiovascular consequences of IUGR do not disappear in adulthood and can manifest themselves in pathological alterations of vasomotor control. The hypothesis was tested that IUGR weakens anticontractile influence of NO and augments procontractile influence of Rho-kinase in arteries of adult offspring. To model IUGR in the rat, dams were 50% food restricted starting from the gestational day 11 till delivery. Mesenteric and coronary arteries of male offspring were studied at the age of 3 months using wire myography, qPCR, and Western blotting. Contractile responses of mesenteric arteries to α(1)-adrenoceptor agonist methoxamine as well as influences of NO and Rho-kinase did not differ between control and IUGR rats. However, coronary arteries of IUGR rats demonstrated elevated contraction to thromboxane A2 receptor agonist U46619 due to weakened anticontractile influence of NO and enhanced role of Rho-kinase in the endothelium. This was accompanied by reduced abundance of SODI protein and elevated content of RhoA protein in coronary arteries of IUGR rats. IUGR considerably changes the regulation of coronary vascular tone in adulthood and, therefore, can serve as a risk factor for the development of cardiac disorders. Nature Publishing Group UK 2021-07-14 /pmc/articles/PMC8280217/ /pubmed/34262070 http://dx.doi.org/10.1038/s41598-021-93491-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Selivanova, Ekaterina K.
Shvetsova, Anastasia A.
Shilova, Lyubov D.
Tarasova, Olga S.
Gaynullina, Dina K.
Intrauterine growth restriction weakens anticontractile influence of NO in coronary arteries of adult rats
title Intrauterine growth restriction weakens anticontractile influence of NO in coronary arteries of adult rats
title_full Intrauterine growth restriction weakens anticontractile influence of NO in coronary arteries of adult rats
title_fullStr Intrauterine growth restriction weakens anticontractile influence of NO in coronary arteries of adult rats
title_full_unstemmed Intrauterine growth restriction weakens anticontractile influence of NO in coronary arteries of adult rats
title_short Intrauterine growth restriction weakens anticontractile influence of NO in coronary arteries of adult rats
title_sort intrauterine growth restriction weakens anticontractile influence of no in coronary arteries of adult rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280217/
https://www.ncbi.nlm.nih.gov/pubmed/34262070
http://dx.doi.org/10.1038/s41598-021-93491-3
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