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Identification of Proteomic Signatures in Chronic Obstructive Pulmonary Disease Emphysematous Phenotype
Chronic obstructive pulmonary disease (COPD) is a highly heterogeneous disease. Emphysematous phenotype is the most common and critical phenotype, which is characterized by progressive lung destruction and poor prognosis. However, the underlying mechanism of this structural damage has not been compl...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280333/ https://www.ncbi.nlm.nih.gov/pubmed/34277700 http://dx.doi.org/10.3389/fmolb.2021.650604 |
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author | Bai, Shuang Ye, Rui Wang, Cuihong Sun, Pengbo Wang, Di Yue, Yong Wang, Huiying Wu, Si Yu, Miao Xi, Shuhua Zhao, Li |
author_facet | Bai, Shuang Ye, Rui Wang, Cuihong Sun, Pengbo Wang, Di Yue, Yong Wang, Huiying Wu, Si Yu, Miao Xi, Shuhua Zhao, Li |
author_sort | Bai, Shuang |
collection | PubMed |
description | Chronic obstructive pulmonary disease (COPD) is a highly heterogeneous disease. Emphysematous phenotype is the most common and critical phenotype, which is characterized by progressive lung destruction and poor prognosis. However, the underlying mechanism of this structural damage has not been completely elucidated. A total of 12 patients with COPD emphysematous phenotype (COPD-E) and nine patients with COPD non-emphysematous phenotype (COPD-NE) were enrolled to determine differences in differential abundant protein (DAP) expression between both groups. Quantitative tandem mass tag–based proteomics was performed on lung tissue samples of all patients. A total of 29 and 15 lung tissue samples from patients in COPD-E and COPD-NE groups, respectively, were used as the validation cohort to verify the proteomic analysis results using western blotting. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted for DAPs. A total of 4,343 proteins were identified, of which 25 were upregulated and 11 were downregulated in the COPD-E group. GO and KEGG analyses showed that wound repair and retinol metabolism–related pathways play an essential role in the molecular mechanism of COPD emphysematous phenotype. Three proteins, namely, KRT17, DHRS9, and FMO3, were selected for validation. While KRT17 and DHRS9 were highly expressed in the lung tissue samples of the COPD-E group, FMO3 expression was not significantly different between both groups. In conclusion, KRT17 and DHRS9 are highly expressed in the lung tissue of patients with COPD emphysematous phenotype. Therefore, these proteins might involve in wound healing and retinol metabolism in patients with emphysematous phenotype and can be used as phenotype-specific markers. |
format | Online Article Text |
id | pubmed-8280333 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82803332021-07-16 Identification of Proteomic Signatures in Chronic Obstructive Pulmonary Disease Emphysematous Phenotype Bai, Shuang Ye, Rui Wang, Cuihong Sun, Pengbo Wang, Di Yue, Yong Wang, Huiying Wu, Si Yu, Miao Xi, Shuhua Zhao, Li Front Mol Biosci Molecular Biosciences Chronic obstructive pulmonary disease (COPD) is a highly heterogeneous disease. Emphysematous phenotype is the most common and critical phenotype, which is characterized by progressive lung destruction and poor prognosis. However, the underlying mechanism of this structural damage has not been completely elucidated. A total of 12 patients with COPD emphysematous phenotype (COPD-E) and nine patients with COPD non-emphysematous phenotype (COPD-NE) were enrolled to determine differences in differential abundant protein (DAP) expression between both groups. Quantitative tandem mass tag–based proteomics was performed on lung tissue samples of all patients. A total of 29 and 15 lung tissue samples from patients in COPD-E and COPD-NE groups, respectively, were used as the validation cohort to verify the proteomic analysis results using western blotting. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted for DAPs. A total of 4,343 proteins were identified, of which 25 were upregulated and 11 were downregulated in the COPD-E group. GO and KEGG analyses showed that wound repair and retinol metabolism–related pathways play an essential role in the molecular mechanism of COPD emphysematous phenotype. Three proteins, namely, KRT17, DHRS9, and FMO3, were selected for validation. While KRT17 and DHRS9 were highly expressed in the lung tissue samples of the COPD-E group, FMO3 expression was not significantly different between both groups. In conclusion, KRT17 and DHRS9 are highly expressed in the lung tissue of patients with COPD emphysematous phenotype. Therefore, these proteins might involve in wound healing and retinol metabolism in patients with emphysematous phenotype and can be used as phenotype-specific markers. Frontiers Media S.A. 2021-07-01 /pmc/articles/PMC8280333/ /pubmed/34277700 http://dx.doi.org/10.3389/fmolb.2021.650604 Text en Copyright © 2021 Bai, Ye, Wang, Sun, Wang, Yue, Wang, Wu, Yu, Xi and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Molecular Biosciences Bai, Shuang Ye, Rui Wang, Cuihong Sun, Pengbo Wang, Di Yue, Yong Wang, Huiying Wu, Si Yu, Miao Xi, Shuhua Zhao, Li Identification of Proteomic Signatures in Chronic Obstructive Pulmonary Disease Emphysematous Phenotype |
title | Identification of Proteomic Signatures in Chronic Obstructive Pulmonary Disease Emphysematous Phenotype |
title_full | Identification of Proteomic Signatures in Chronic Obstructive Pulmonary Disease Emphysematous Phenotype |
title_fullStr | Identification of Proteomic Signatures in Chronic Obstructive Pulmonary Disease Emphysematous Phenotype |
title_full_unstemmed | Identification of Proteomic Signatures in Chronic Obstructive Pulmonary Disease Emphysematous Phenotype |
title_short | Identification of Proteomic Signatures in Chronic Obstructive Pulmonary Disease Emphysematous Phenotype |
title_sort | identification of proteomic signatures in chronic obstructive pulmonary disease emphysematous phenotype |
topic | Molecular Biosciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280333/ https://www.ncbi.nlm.nih.gov/pubmed/34277700 http://dx.doi.org/10.3389/fmolb.2021.650604 |
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