Synthesis of Silica Nanoparticles with Physical Encapsulation of Near-Infrared Fluorescent Dyes and Their Tannic Acid Coating

[Image: see text] This study reports a novel method for the synthesis of silica nanoparticles (NPs) encapsulating near-infrared (NIR) fluorescent dyes through physical adsorption. Although a NIR cationic fluorescent dye, oxazine 725 (OXA), has no chemical bonding moiety toward silica NPs such as the triethoxysilyl group, the dyes were successfully incorporated into silica NPs without denaturation under the mild reaction conditions. Next, tannic acid (TA) molecules were coated in the presence of Fe(3+) on the particle surface for the functionalization of silica NPs encapsulating OXA (OXA@SiO(2) NPs). The TA coating on the surface of OXA@SiO(2) NPs was confirmed by transmission electron microscopy and X-ray photoelectron spectroscopy. The TA coating significantly contributed to the resistance improvement against photobleaching and leakage of the dyes in the NPs. Furthermore, the obtained TA-coated silica NPs encapsulating OXAs (OXA@SiO(2)@TA NPs) were used for the fluorescence imaging of African green monkey kidney (COS-7) cells, and it was shown that the fluorescence originated from OXA@SiO(2)@TA NPs was clearly observed in the COS-7 cells.