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Structural and Functional Analysis of a Multimodular Hyperthermostable Xylanase-Glucuronoyl Esterase from Caldicellulosiruptor kristjansonii
[Image: see text] The hyperthermophilic bacterium Caldicellulosiruptor kristjansonii encodes an unusual enzyme, CkXyn10C-GE15A, which incorporates two catalytic domains, a xylanase and a glucuronoyl esterase, and five carbohydrate-binding modules (CBMs) from families 9 and 22. The xylanase and glucu...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280721/ https://www.ncbi.nlm.nih.gov/pubmed/34180241 http://dx.doi.org/10.1021/acs.biochem.1c00305 |
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author | Krska, Daniel Mazurkewich, Scott Brown, Haley A. Theibich, Yusuf Poulsen, Jens-Christian N. Morris, Adeline L. Koropatkin, Nicole M. Lo Leggio, Leila Larsbrink, Johan |
author_facet | Krska, Daniel Mazurkewich, Scott Brown, Haley A. Theibich, Yusuf Poulsen, Jens-Christian N. Morris, Adeline L. Koropatkin, Nicole M. Lo Leggio, Leila Larsbrink, Johan |
author_sort | Krska, Daniel |
collection | PubMed |
description | [Image: see text] The hyperthermophilic bacterium Caldicellulosiruptor kristjansonii encodes an unusual enzyme, CkXyn10C-GE15A, which incorporates two catalytic domains, a xylanase and a glucuronoyl esterase, and five carbohydrate-binding modules (CBMs) from families 9 and 22. The xylanase and glucuronoyl esterase catalytic domains were recently biochemically characterized, as was the ability of the individual CBMs to bind insoluble polysaccharides. Here, we further probed the abilities of the different CBMs from CkXyn10C-GE15A to bind to soluble poly- and oligosaccharides using affinity gel electrophoresis, isothermal titration calorimetry, and differential scanning fluorimetry. The results revealed additional binding properties of the proteins compared to the former studies on insoluble polysaccharides. Collectively, the results show that all five CBMs have their own distinct binding preferences and appear to complement each other and the catalytic domains in targeting complex cell wall polysaccharides. Additionally, through renewed efforts, we have achieved partial structural characterization of this complex multidomain protein. We have determined the structures of the third CBM9 domain (CBM9.3) and the glucuronoyl esterase (GE15A) by X-ray crystallography. CBM9.3 is the second CBM9 structure determined to date and was shown to bind oligosaccharide ligands at the same site but in a different binding mode compared to that of the previously determined CBM9 structure from Thermotoga maritima. GE15A represents a unique intermediate between reported fungal and bacterial glucuronoyl esterase structures as it lacks two inserted loop regions typical of bacterial enzymes and a third loop has an atypical structure. We also report small-angle X-ray scattering measurements of the N-terminal CBM22.1–CBM22.2–Xyn10C construct, indicating a compact arrangement at room temperature. |
format | Online Article Text |
id | pubmed-8280721 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-82807212021-07-16 Structural and Functional Analysis of a Multimodular Hyperthermostable Xylanase-Glucuronoyl Esterase from Caldicellulosiruptor kristjansonii Krska, Daniel Mazurkewich, Scott Brown, Haley A. Theibich, Yusuf Poulsen, Jens-Christian N. Morris, Adeline L. Koropatkin, Nicole M. Lo Leggio, Leila Larsbrink, Johan Biochemistry [Image: see text] The hyperthermophilic bacterium Caldicellulosiruptor kristjansonii encodes an unusual enzyme, CkXyn10C-GE15A, which incorporates two catalytic domains, a xylanase and a glucuronoyl esterase, and five carbohydrate-binding modules (CBMs) from families 9 and 22. The xylanase and glucuronoyl esterase catalytic domains were recently biochemically characterized, as was the ability of the individual CBMs to bind insoluble polysaccharides. Here, we further probed the abilities of the different CBMs from CkXyn10C-GE15A to bind to soluble poly- and oligosaccharides using affinity gel electrophoresis, isothermal titration calorimetry, and differential scanning fluorimetry. The results revealed additional binding properties of the proteins compared to the former studies on insoluble polysaccharides. Collectively, the results show that all five CBMs have their own distinct binding preferences and appear to complement each other and the catalytic domains in targeting complex cell wall polysaccharides. Additionally, through renewed efforts, we have achieved partial structural characterization of this complex multidomain protein. We have determined the structures of the third CBM9 domain (CBM9.3) and the glucuronoyl esterase (GE15A) by X-ray crystallography. CBM9.3 is the second CBM9 structure determined to date and was shown to bind oligosaccharide ligands at the same site but in a different binding mode compared to that of the previously determined CBM9 structure from Thermotoga maritima. GE15A represents a unique intermediate between reported fungal and bacterial glucuronoyl esterase structures as it lacks two inserted loop regions typical of bacterial enzymes and a third loop has an atypical structure. We also report small-angle X-ray scattering measurements of the N-terminal CBM22.1–CBM22.2–Xyn10C construct, indicating a compact arrangement at room temperature. American Chemical Society 2021-06-28 2021-07-13 /pmc/articles/PMC8280721/ /pubmed/34180241 http://dx.doi.org/10.1021/acs.biochem.1c00305 Text en © 2021 The Authors. Published by American Chemical Society Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Krska, Daniel Mazurkewich, Scott Brown, Haley A. Theibich, Yusuf Poulsen, Jens-Christian N. Morris, Adeline L. Koropatkin, Nicole M. Lo Leggio, Leila Larsbrink, Johan Structural and Functional Analysis of a Multimodular Hyperthermostable Xylanase-Glucuronoyl Esterase from Caldicellulosiruptor kristjansonii |
title | Structural and Functional Analysis of a Multimodular
Hyperthermostable Xylanase-Glucuronoyl Esterase from Caldicellulosiruptor
kristjansonii |
title_full | Structural and Functional Analysis of a Multimodular
Hyperthermostable Xylanase-Glucuronoyl Esterase from Caldicellulosiruptor
kristjansonii |
title_fullStr | Structural and Functional Analysis of a Multimodular
Hyperthermostable Xylanase-Glucuronoyl Esterase from Caldicellulosiruptor
kristjansonii |
title_full_unstemmed | Structural and Functional Analysis of a Multimodular
Hyperthermostable Xylanase-Glucuronoyl Esterase from Caldicellulosiruptor
kristjansonii |
title_short | Structural and Functional Analysis of a Multimodular
Hyperthermostable Xylanase-Glucuronoyl Esterase from Caldicellulosiruptor
kristjansonii |
title_sort | structural and functional analysis of a multimodular
hyperthermostable xylanase-glucuronoyl esterase from caldicellulosiruptor
kristjansonii |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280721/ https://www.ncbi.nlm.nih.gov/pubmed/34180241 http://dx.doi.org/10.1021/acs.biochem.1c00305 |
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