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Scavenger Receptor BI Induced by HDL From Coronary Heart Disease May Be Related to Atherosclerosis
This study aims to determine whether dysfunctional High Density Lipoprotein (HDL) influenced the expression of scavenger receptor class B type Ⅰ (SR-B1) to determine reverse cholesterol transport. Blood samples obtained from coronary heart disease patients confirmed by angiography were collected. HD...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280836/ https://www.ncbi.nlm.nih.gov/pubmed/34254531 http://dx.doi.org/10.1177/10760296211029710 |
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author | Gao, Fen Feng, Gao-jie Li, Hong Qin, Wei-wei Xiao, Chuan-shi |
author_facet | Gao, Fen Feng, Gao-jie Li, Hong Qin, Wei-wei Xiao, Chuan-shi |
author_sort | Gao, Fen |
collection | PubMed |
description | This study aims to determine whether dysfunctional High Density Lipoprotein (HDL) influenced the expression of scavenger receptor class B type Ⅰ (SR-B1) to determine reverse cholesterol transport. Blood samples obtained from coronary heart disease patients confirmed by angiography were collected. HDL was extracted from the blood via ultracentrifugation. Then, the HDL was injected into apoE−/− mice, and the HepG2 cells cultured with Dulbecco’s modified eagle medium (DMEM) were added the HDL extracted from coronary heart disease patients. As controls, normal cases without coronary heart disease (CHD) and patients with angina pectoris and acute myocardial infarction were used. The protein expression levels of SR-B1 were detected by western blot, and the lipid accumulation levels were detected by Oil Red O staining in both tissues and cell levels. These results revealed that the HDL obtained from CHD patients downregulate the SR-B1 expression in ex vitro and in vitro studies. In addition, dysfunctional HDL may result in lower SR-B1 expression levels. The degree of SR-B1 expression levels could be relative to the degree of coronary congestion. Along with the increase in severe coronary congestion, such as myocardial infarction, the SR-B1 expression levels were lower. The dysfunctional HDL derived from coronary heart disease patients decreased the expression of SR-B1, and promoted lipid accumulation. |
format | Online Article Text |
id | pubmed-8280836 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-82808362021-08-02 Scavenger Receptor BI Induced by HDL From Coronary Heart Disease May Be Related to Atherosclerosis Gao, Fen Feng, Gao-jie Li, Hong Qin, Wei-wei Xiao, Chuan-shi Clin Appl Thromb Hemost Original Manuscript This study aims to determine whether dysfunctional High Density Lipoprotein (HDL) influenced the expression of scavenger receptor class B type Ⅰ (SR-B1) to determine reverse cholesterol transport. Blood samples obtained from coronary heart disease patients confirmed by angiography were collected. HDL was extracted from the blood via ultracentrifugation. Then, the HDL was injected into apoE−/− mice, and the HepG2 cells cultured with Dulbecco’s modified eagle medium (DMEM) were added the HDL extracted from coronary heart disease patients. As controls, normal cases without coronary heart disease (CHD) and patients with angina pectoris and acute myocardial infarction were used. The protein expression levels of SR-B1 were detected by western blot, and the lipid accumulation levels were detected by Oil Red O staining in both tissues and cell levels. These results revealed that the HDL obtained from CHD patients downregulate the SR-B1 expression in ex vitro and in vitro studies. In addition, dysfunctional HDL may result in lower SR-B1 expression levels. The degree of SR-B1 expression levels could be relative to the degree of coronary congestion. Along with the increase in severe coronary congestion, such as myocardial infarction, the SR-B1 expression levels were lower. The dysfunctional HDL derived from coronary heart disease patients decreased the expression of SR-B1, and promoted lipid accumulation. SAGE Publications 2021-07-13 /pmc/articles/PMC8280836/ /pubmed/34254531 http://dx.doi.org/10.1177/10760296211029710 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Manuscript Gao, Fen Feng, Gao-jie Li, Hong Qin, Wei-wei Xiao, Chuan-shi Scavenger Receptor BI Induced by HDL From Coronary Heart Disease May Be Related to Atherosclerosis |
title | Scavenger Receptor BI Induced by HDL From Coronary Heart
Disease May Be Related to Atherosclerosis |
title_full | Scavenger Receptor BI Induced by HDL From Coronary Heart
Disease May Be Related to Atherosclerosis |
title_fullStr | Scavenger Receptor BI Induced by HDL From Coronary Heart
Disease May Be Related to Atherosclerosis |
title_full_unstemmed | Scavenger Receptor BI Induced by HDL From Coronary Heart
Disease May Be Related to Atherosclerosis |
title_short | Scavenger Receptor BI Induced by HDL From Coronary Heart
Disease May Be Related to Atherosclerosis |
title_sort | scavenger receptor bi induced by hdl from coronary heart
disease may be related to atherosclerosis |
topic | Original Manuscript |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280836/ https://www.ncbi.nlm.nih.gov/pubmed/34254531 http://dx.doi.org/10.1177/10760296211029710 |
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