Imidazolium labelling permits the sensitive mass-spectrometric detection of N-glycosides directly from serum

A novel imidazolium derivative (GITag) shows superior ionisation and consequently allows increased mass spectrometric detection capabilities of oligosaccharides and N-glycans. Here we demonstrate that human serum samples can be directly labelled by GITag on a MALDI target plate, abrogating prevalent...

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Detalles Bibliográficos
Autores principales: Zhang, Yao-Yao, Ghirardello, Mattia, Wang, Ting, Lu, Ai-Min, Liu, Li, Voglmeir, Josef, Galan, M. Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280963/
https://www.ncbi.nlm.nih.gov/pubmed/34159978
http://dx.doi.org/10.1039/d1cc02100a
Descripción
Sumario:A novel imidazolium derivative (GITag) shows superior ionisation and consequently allows increased mass spectrometric detection capabilities of oligosaccharides and N-glycans. Here we demonstrate that human serum samples can be directly labelled by GITag on a MALDI target plate, abrogating prevalently required sample pretreatment or clean-up steps.

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