Imidazolium labelling permits the sensitive mass-spectrometric detection of N-glycosides directly from serum

A novel imidazolium derivative (GITag) shows superior ionisation and consequently allows increased mass spectrometric detection capabilities of oligosaccharides and N-glycans. Here we demonstrate that human serum samples can be directly labelled by GITag on a MALDI target plate, abrogating prevalent...

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Detalles Bibliográficos
Autores principales: Zhang, Yao-Yao, Ghirardello, Mattia, Wang, Ting, Lu, Ai-Min, Liu, Li, Voglmeir, Josef, Galan, M. Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280963/
https://www.ncbi.nlm.nih.gov/pubmed/34159978
http://dx.doi.org/10.1039/d1cc02100a
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author Zhang, Yao-Yao
Ghirardello, Mattia
Wang, Ting
Lu, Ai-Min
Liu, Li
Voglmeir, Josef
Galan, M. Carmen
author_facet Zhang, Yao-Yao
Ghirardello, Mattia
Wang, Ting
Lu, Ai-Min
Liu, Li
Voglmeir, Josef
Galan, M. Carmen
author_sort Zhang, Yao-Yao
collection PubMed
description A novel imidazolium derivative (GITag) shows superior ionisation and consequently allows increased mass spectrometric detection capabilities of oligosaccharides and N-glycans. Here we demonstrate that human serum samples can be directly labelled by GITag on a MALDI target plate, abrogating prevalently required sample pretreatment or clean-up steps.
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spelling pubmed-82809632021-08-03 Imidazolium labelling permits the sensitive mass-spectrometric detection of N-glycosides directly from serum Zhang, Yao-Yao Ghirardello, Mattia Wang, Ting Lu, Ai-Min Liu, Li Voglmeir, Josef Galan, M. Carmen Chem Commun (Camb) Chemistry A novel imidazolium derivative (GITag) shows superior ionisation and consequently allows increased mass spectrometric detection capabilities of oligosaccharides and N-glycans. Here we demonstrate that human serum samples can be directly labelled by GITag on a MALDI target plate, abrogating prevalently required sample pretreatment or clean-up steps. The Royal Society of Chemistry 2021-06-17 /pmc/articles/PMC8280963/ /pubmed/34159978 http://dx.doi.org/10.1039/d1cc02100a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Zhang, Yao-Yao
Ghirardello, Mattia
Wang, Ting
Lu, Ai-Min
Liu, Li
Voglmeir, Josef
Galan, M. Carmen
Imidazolium labelling permits the sensitive mass-spectrometric detection of N-glycosides directly from serum
title Imidazolium labelling permits the sensitive mass-spectrometric detection of N-glycosides directly from serum
title_full Imidazolium labelling permits the sensitive mass-spectrometric detection of N-glycosides directly from serum
title_fullStr Imidazolium labelling permits the sensitive mass-spectrometric detection of N-glycosides directly from serum
title_full_unstemmed Imidazolium labelling permits the sensitive mass-spectrometric detection of N-glycosides directly from serum
title_short Imidazolium labelling permits the sensitive mass-spectrometric detection of N-glycosides directly from serum
title_sort imidazolium labelling permits the sensitive mass-spectrometric detection of n-glycosides directly from serum
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280963/
https://www.ncbi.nlm.nih.gov/pubmed/34159978
http://dx.doi.org/10.1039/d1cc02100a
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