Cargando…

Identification of a Genetically Linked but Functionally Independent Two-Component System Important for Cell Division of the Rice Pathogen Burkholderia glumae

Bacterial two-component regulatory systems control the expression of sets of genes to coordinate physiological functions in response to environmental cues. Here, we report a genetically linked but functionally unpaired two-component system (TCS) comprising the sensor kinase GluS (BGLU_1G13350) and t...

Descripción completa

Detalles Bibliográficos
Autores principales: Marunga, Joan, Goo, Eunhye, Kang, Yongsung, Hwang, Ingyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281045/
https://www.ncbi.nlm.nih.gov/pubmed/34276634
http://dx.doi.org/10.3389/fmicb.2021.700333
_version_ 1783722766846918656
author Marunga, Joan
Goo, Eunhye
Kang, Yongsung
Hwang, Ingyu
author_facet Marunga, Joan
Goo, Eunhye
Kang, Yongsung
Hwang, Ingyu
author_sort Marunga, Joan
collection PubMed
description Bacterial two-component regulatory systems control the expression of sets of genes to coordinate physiological functions in response to environmental cues. Here, we report a genetically linked but functionally unpaired two-component system (TCS) comprising the sensor kinase GluS (BGLU_1G13350) and the response regulator GluR (BGLU_1G13360), which is critical for cell division in the rice pathogen Burkholderia glumae BGR1. The gluR null mutant, unlike the gluS mutant, formed filamentous cells in Lysogeny Broth medium and was sensitive to exposure to 42°C. Expression of genes responsible for cell division and cell-wall (dcw) biosynthesis in the gluR mutant was elevated at transcription levels compared with the wild type. GluR-His bound to the putative promoter regions of ftsA and ftsZ is involved in septum formation, indicating that repression of genes in the dcw cluster by GluR is critical for cell division in B. glumae. The gluR mutant did not form filamentous cells in M9 minimal medium, whereas exogenous addition of glutamine or glutamate to the medium induced filamentous cell formation. These results indicate that glutamine and glutamate influence GluR-mediated cell division in B. glumae, suggesting that GluR controls cell division of B. glumae in a nutrition-dependent manner. These findings provide insight into how the recognition of external signals by TCS affects the sophisticated molecular mechanisms involved in controlling bacterial cell division.
format Online
Article
Text
id pubmed-8281045
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-82810452021-07-16 Identification of a Genetically Linked but Functionally Independent Two-Component System Important for Cell Division of the Rice Pathogen Burkholderia glumae Marunga, Joan Goo, Eunhye Kang, Yongsung Hwang, Ingyu Front Microbiol Microbiology Bacterial two-component regulatory systems control the expression of sets of genes to coordinate physiological functions in response to environmental cues. Here, we report a genetically linked but functionally unpaired two-component system (TCS) comprising the sensor kinase GluS (BGLU_1G13350) and the response regulator GluR (BGLU_1G13360), which is critical for cell division in the rice pathogen Burkholderia glumae BGR1. The gluR null mutant, unlike the gluS mutant, formed filamentous cells in Lysogeny Broth medium and was sensitive to exposure to 42°C. Expression of genes responsible for cell division and cell-wall (dcw) biosynthesis in the gluR mutant was elevated at transcription levels compared with the wild type. GluR-His bound to the putative promoter regions of ftsA and ftsZ is involved in septum formation, indicating that repression of genes in the dcw cluster by GluR is critical for cell division in B. glumae. The gluR mutant did not form filamentous cells in M9 minimal medium, whereas exogenous addition of glutamine or glutamate to the medium induced filamentous cell formation. These results indicate that glutamine and glutamate influence GluR-mediated cell division in B. glumae, suggesting that GluR controls cell division of B. glumae in a nutrition-dependent manner. These findings provide insight into how the recognition of external signals by TCS affects the sophisticated molecular mechanisms involved in controlling bacterial cell division. Frontiers Media S.A. 2021-07-01 /pmc/articles/PMC8281045/ /pubmed/34276634 http://dx.doi.org/10.3389/fmicb.2021.700333 Text en Copyright © 2021 Marunga, Goo, Kang and Hwang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Marunga, Joan
Goo, Eunhye
Kang, Yongsung
Hwang, Ingyu
Identification of a Genetically Linked but Functionally Independent Two-Component System Important for Cell Division of the Rice Pathogen Burkholderia glumae
title Identification of a Genetically Linked but Functionally Independent Two-Component System Important for Cell Division of the Rice Pathogen Burkholderia glumae
title_full Identification of a Genetically Linked but Functionally Independent Two-Component System Important for Cell Division of the Rice Pathogen Burkholderia glumae
title_fullStr Identification of a Genetically Linked but Functionally Independent Two-Component System Important for Cell Division of the Rice Pathogen Burkholderia glumae
title_full_unstemmed Identification of a Genetically Linked but Functionally Independent Two-Component System Important for Cell Division of the Rice Pathogen Burkholderia glumae
title_short Identification of a Genetically Linked but Functionally Independent Two-Component System Important for Cell Division of the Rice Pathogen Burkholderia glumae
title_sort identification of a genetically linked but functionally independent two-component system important for cell division of the rice pathogen burkholderia glumae
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281045/
https://www.ncbi.nlm.nih.gov/pubmed/34276634
http://dx.doi.org/10.3389/fmicb.2021.700333
work_keys_str_mv AT marungajoan identificationofageneticallylinkedbutfunctionallyindependenttwocomponentsystemimportantforcelldivisionofthericepathogenburkholderiaglumae
AT gooeunhye identificationofageneticallylinkedbutfunctionallyindependenttwocomponentsystemimportantforcelldivisionofthericepathogenburkholderiaglumae
AT kangyongsung identificationofageneticallylinkedbutfunctionallyindependenttwocomponentsystemimportantforcelldivisionofthericepathogenburkholderiaglumae
AT hwangingyu identificationofageneticallylinkedbutfunctionallyindependenttwocomponentsystemimportantforcelldivisionofthericepathogenburkholderiaglumae