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Near-Complete Genome Sequence of a Human Norovirus GII.1[Pg] Strain Associated with Acute Gastroenteritis, Determined Using Long-Read Sequencing
High-throughput sequencing is one of the approaches used for the detection of foodborne pathogens such as noroviruses. Long-read sequencing has advantages over short-read sequencing in speed, read length, and lower fragmentation bias, which makes it a potential powerful tool for the fast detection a...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281079/ https://www.ncbi.nlm.nih.gov/pubmed/34264118 http://dx.doi.org/10.1128/MRA.00401-21 |
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author | Yang, Zhihui Mammel, Mark Wales, Samantha Q. |
author_facet | Yang, Zhihui Mammel, Mark Wales, Samantha Q. |
author_sort | Yang, Zhihui |
collection | PubMed |
description | High-throughput sequencing is one of the approaches used for the detection of foodborne pathogens such as noroviruses. Long-read sequencing has advantages over short-read sequencing in speed, read length, and lower fragmentation bias, which makes it a potential powerful tool for the fast detection and identification of viruses. Using Nanopore sequencing technology, we were able to successfully recover a nearly complete genome sequence of a human norovirus GII.1[Pg] strain in a single long read from a sample from a patient with norovirus gastroenteritis. |
format | Online Article Text |
id | pubmed-8281079 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-82810792021-08-02 Near-Complete Genome Sequence of a Human Norovirus GII.1[Pg] Strain Associated with Acute Gastroenteritis, Determined Using Long-Read Sequencing Yang, Zhihui Mammel, Mark Wales, Samantha Q. Microbiol Resour Announc Genome Sequences High-throughput sequencing is one of the approaches used for the detection of foodborne pathogens such as noroviruses. Long-read sequencing has advantages over short-read sequencing in speed, read length, and lower fragmentation bias, which makes it a potential powerful tool for the fast detection and identification of viruses. Using Nanopore sequencing technology, we were able to successfully recover a nearly complete genome sequence of a human norovirus GII.1[Pg] strain in a single long read from a sample from a patient with norovirus gastroenteritis. American Society for Microbiology 2021-07-15 /pmc/articles/PMC8281079/ /pubmed/34264118 http://dx.doi.org/10.1128/MRA.00401-21 Text en Copyright © 2021 Yang et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Genome Sequences Yang, Zhihui Mammel, Mark Wales, Samantha Q. Near-Complete Genome Sequence of a Human Norovirus GII.1[Pg] Strain Associated with Acute Gastroenteritis, Determined Using Long-Read Sequencing |
title | Near-Complete Genome Sequence of a Human Norovirus GII.1[Pg] Strain Associated with Acute Gastroenteritis, Determined Using Long-Read Sequencing |
title_full | Near-Complete Genome Sequence of a Human Norovirus GII.1[Pg] Strain Associated with Acute Gastroenteritis, Determined Using Long-Read Sequencing |
title_fullStr | Near-Complete Genome Sequence of a Human Norovirus GII.1[Pg] Strain Associated with Acute Gastroenteritis, Determined Using Long-Read Sequencing |
title_full_unstemmed | Near-Complete Genome Sequence of a Human Norovirus GII.1[Pg] Strain Associated with Acute Gastroenteritis, Determined Using Long-Read Sequencing |
title_short | Near-Complete Genome Sequence of a Human Norovirus GII.1[Pg] Strain Associated with Acute Gastroenteritis, Determined Using Long-Read Sequencing |
title_sort | near-complete genome sequence of a human norovirus gii.1[pg] strain associated with acute gastroenteritis, determined using long-read sequencing |
topic | Genome Sequences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281079/ https://www.ncbi.nlm.nih.gov/pubmed/34264118 http://dx.doi.org/10.1128/MRA.00401-21 |
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