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Near-Complete Genome Sequence of a Human Norovirus GII.1[Pg] Strain Associated with Acute Gastroenteritis, Determined Using Long-Read Sequencing

High-throughput sequencing is one of the approaches used for the detection of foodborne pathogens such as noroviruses. Long-read sequencing has advantages over short-read sequencing in speed, read length, and lower fragmentation bias, which makes it a potential powerful tool for the fast detection a...

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Detalles Bibliográficos
Autores principales: Yang, Zhihui, Mammel, Mark, Wales, Samantha Q.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281079/
https://www.ncbi.nlm.nih.gov/pubmed/34264118
http://dx.doi.org/10.1128/MRA.00401-21
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author Yang, Zhihui
Mammel, Mark
Wales, Samantha Q.
author_facet Yang, Zhihui
Mammel, Mark
Wales, Samantha Q.
author_sort Yang, Zhihui
collection PubMed
description High-throughput sequencing is one of the approaches used for the detection of foodborne pathogens such as noroviruses. Long-read sequencing has advantages over short-read sequencing in speed, read length, and lower fragmentation bias, which makes it a potential powerful tool for the fast detection and identification of viruses. Using Nanopore sequencing technology, we were able to successfully recover a nearly complete genome sequence of a human norovirus GII.1[Pg] strain in a single long read from a sample from a patient with norovirus gastroenteritis.
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spelling pubmed-82810792021-08-02 Near-Complete Genome Sequence of a Human Norovirus GII.1[Pg] Strain Associated with Acute Gastroenteritis, Determined Using Long-Read Sequencing Yang, Zhihui Mammel, Mark Wales, Samantha Q. Microbiol Resour Announc Genome Sequences High-throughput sequencing is one of the approaches used for the detection of foodborne pathogens such as noroviruses. Long-read sequencing has advantages over short-read sequencing in speed, read length, and lower fragmentation bias, which makes it a potential powerful tool for the fast detection and identification of viruses. Using Nanopore sequencing technology, we were able to successfully recover a nearly complete genome sequence of a human norovirus GII.1[Pg] strain in a single long read from a sample from a patient with norovirus gastroenteritis. American Society for Microbiology 2021-07-15 /pmc/articles/PMC8281079/ /pubmed/34264118 http://dx.doi.org/10.1128/MRA.00401-21 Text en Copyright © 2021 Yang et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Genome Sequences
Yang, Zhihui
Mammel, Mark
Wales, Samantha Q.
Near-Complete Genome Sequence of a Human Norovirus GII.1[Pg] Strain Associated with Acute Gastroenteritis, Determined Using Long-Read Sequencing
title Near-Complete Genome Sequence of a Human Norovirus GII.1[Pg] Strain Associated with Acute Gastroenteritis, Determined Using Long-Read Sequencing
title_full Near-Complete Genome Sequence of a Human Norovirus GII.1[Pg] Strain Associated with Acute Gastroenteritis, Determined Using Long-Read Sequencing
title_fullStr Near-Complete Genome Sequence of a Human Norovirus GII.1[Pg] Strain Associated with Acute Gastroenteritis, Determined Using Long-Read Sequencing
title_full_unstemmed Near-Complete Genome Sequence of a Human Norovirus GII.1[Pg] Strain Associated with Acute Gastroenteritis, Determined Using Long-Read Sequencing
title_short Near-Complete Genome Sequence of a Human Norovirus GII.1[Pg] Strain Associated with Acute Gastroenteritis, Determined Using Long-Read Sequencing
title_sort near-complete genome sequence of a human norovirus gii.1[pg] strain associated with acute gastroenteritis, determined using long-read sequencing
topic Genome Sequences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281079/
https://www.ncbi.nlm.nih.gov/pubmed/34264118
http://dx.doi.org/10.1128/MRA.00401-21
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