Cargando…

Leptin Receptor Expressing Neurons in the Substantia Nigra Regulate Locomotion, and in The Ventral Tegmental Area Motivation and Feeding

Leptin is an anorexigenic hormone, important in the regulation of body weight. Leptin plays a role in food reward, feeding, locomotion and anxiety. Leptin receptors (LepR) are expressed in many brain areas, including the midbrain. In most studies that target the midbrain, either all LepR neurons of...

Descripción completa

Detalles Bibliográficos
Autores principales: de Vrind, Véronne A. J., van ‘t Sant, Lisanne J., Rozeboom, Annemieke, Luijendijk-Berg, Mieneke C. M., Omrani, Azar, Adan, Roger A. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281287/
https://www.ncbi.nlm.nih.gov/pubmed/34276560
http://dx.doi.org/10.3389/fendo.2021.680494
_version_ 1783722818506063872
author de Vrind, Véronne A. J.
van ‘t Sant, Lisanne J.
Rozeboom, Annemieke
Luijendijk-Berg, Mieneke C. M.
Omrani, Azar
Adan, Roger A. H.
author_facet de Vrind, Véronne A. J.
van ‘t Sant, Lisanne J.
Rozeboom, Annemieke
Luijendijk-Berg, Mieneke C. M.
Omrani, Azar
Adan, Roger A. H.
author_sort de Vrind, Véronne A. J.
collection PubMed
description Leptin is an anorexigenic hormone, important in the regulation of body weight. Leptin plays a role in food reward, feeding, locomotion and anxiety. Leptin receptors (LepR) are expressed in many brain areas, including the midbrain. In most studies that target the midbrain, either all LepR neurons of the midbrain or those of the ventral tegmental area (VTA) were targeted, but the role of substantia nigra (SN) LepR neurons has not been investigated. These studies have reported contradicting results regarding motivational behavior for food reward, feeding and locomotion. Since not all midbrain LepR mediated behaviors can be explained by LepR neurons in the VTA alone, we hypothesized that SN LepR neurons may provide further insight. We first characterized SN LepR and VTA LepR expression, which revealed LepR expression mainly on DA neurons. To further understand the role of midbrain LepR neurons in body weight regulation, we chemogenetically activated VTA LepR or SN LepR neurons in LepR-cre mice and tested for motivational behavior, feeding and locomotion. Activation of VTA LepR neurons in food restricted mice decreased motivation for food reward (p=0.032) and food intake (p=0.020), but not locomotion. In contrast, activation of SN LepR neurons in food restricted mice decreased locomotion (p=0.025), but not motivation for food reward or food intake. Our results provide evidence that VTA LepR and SN LepR neurons serve different functions, i.e. activation of VTA LepR neurons modulated motivation for food reward and feeding, while SN LepR neurons modulated locomotor activity.
format Online
Article
Text
id pubmed-8281287
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-82812872021-07-16 Leptin Receptor Expressing Neurons in the Substantia Nigra Regulate Locomotion, and in The Ventral Tegmental Area Motivation and Feeding de Vrind, Véronne A. J. van ‘t Sant, Lisanne J. Rozeboom, Annemieke Luijendijk-Berg, Mieneke C. M. Omrani, Azar Adan, Roger A. H. Front Endocrinol (Lausanne) Endocrinology Leptin is an anorexigenic hormone, important in the regulation of body weight. Leptin plays a role in food reward, feeding, locomotion and anxiety. Leptin receptors (LepR) are expressed in many brain areas, including the midbrain. In most studies that target the midbrain, either all LepR neurons of the midbrain or those of the ventral tegmental area (VTA) were targeted, but the role of substantia nigra (SN) LepR neurons has not been investigated. These studies have reported contradicting results regarding motivational behavior for food reward, feeding and locomotion. Since not all midbrain LepR mediated behaviors can be explained by LepR neurons in the VTA alone, we hypothesized that SN LepR neurons may provide further insight. We first characterized SN LepR and VTA LepR expression, which revealed LepR expression mainly on DA neurons. To further understand the role of midbrain LepR neurons in body weight regulation, we chemogenetically activated VTA LepR or SN LepR neurons in LepR-cre mice and tested for motivational behavior, feeding and locomotion. Activation of VTA LepR neurons in food restricted mice decreased motivation for food reward (p=0.032) and food intake (p=0.020), but not locomotion. In contrast, activation of SN LepR neurons in food restricted mice decreased locomotion (p=0.025), but not motivation for food reward or food intake. Our results provide evidence that VTA LepR and SN LepR neurons serve different functions, i.e. activation of VTA LepR neurons modulated motivation for food reward and feeding, while SN LepR neurons modulated locomotor activity. Frontiers Media S.A. 2021-07-01 /pmc/articles/PMC8281287/ /pubmed/34276560 http://dx.doi.org/10.3389/fendo.2021.680494 Text en Copyright © 2021 de Vrind, van ‘t Sant, Rozeboom, Luijendijk-Berg, Omrani and Adan https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
de Vrind, Véronne A. J.
van ‘t Sant, Lisanne J.
Rozeboom, Annemieke
Luijendijk-Berg, Mieneke C. M.
Omrani, Azar
Adan, Roger A. H.
Leptin Receptor Expressing Neurons in the Substantia Nigra Regulate Locomotion, and in The Ventral Tegmental Area Motivation and Feeding
title Leptin Receptor Expressing Neurons in the Substantia Nigra Regulate Locomotion, and in The Ventral Tegmental Area Motivation and Feeding
title_full Leptin Receptor Expressing Neurons in the Substantia Nigra Regulate Locomotion, and in The Ventral Tegmental Area Motivation and Feeding
title_fullStr Leptin Receptor Expressing Neurons in the Substantia Nigra Regulate Locomotion, and in The Ventral Tegmental Area Motivation and Feeding
title_full_unstemmed Leptin Receptor Expressing Neurons in the Substantia Nigra Regulate Locomotion, and in The Ventral Tegmental Area Motivation and Feeding
title_short Leptin Receptor Expressing Neurons in the Substantia Nigra Regulate Locomotion, and in The Ventral Tegmental Area Motivation and Feeding
title_sort leptin receptor expressing neurons in the substantia nigra regulate locomotion, and in the ventral tegmental area motivation and feeding
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281287/
https://www.ncbi.nlm.nih.gov/pubmed/34276560
http://dx.doi.org/10.3389/fendo.2021.680494
work_keys_str_mv AT devrindveronneaj leptinreceptorexpressingneuronsinthesubstantianigraregulatelocomotionandintheventraltegmentalareamotivationandfeeding
AT vantsantlisannej leptinreceptorexpressingneuronsinthesubstantianigraregulatelocomotionandintheventraltegmentalareamotivationandfeeding
AT rozeboomannemieke leptinreceptorexpressingneuronsinthesubstantianigraregulatelocomotionandintheventraltegmentalareamotivationandfeeding
AT luijendijkbergmienekecm leptinreceptorexpressingneuronsinthesubstantianigraregulatelocomotionandintheventraltegmentalareamotivationandfeeding
AT omraniazar leptinreceptorexpressingneuronsinthesubstantianigraregulatelocomotionandintheventraltegmentalareamotivationandfeeding
AT adanrogerah leptinreceptorexpressingneuronsinthesubstantianigraregulatelocomotionandintheventraltegmentalareamotivationandfeeding