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Mesenchymal stem cell transplantation for vaginal repair in an ovariectomized rhesus macaque model

BACKGROUND: Current surgical therapies for pelvic organ prolapse (POP) do not repair weak vaginal tissue and just provide support; these therapies may trigger severe complications. Stem cell-based regenerative therapy, due to its ability to reconstruct damaged tissue, may be a promising therapeutic...

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Autores principales: Zhang, Ye, Ma, Yidi, Chen, Juan, Wang, Min, Cao, Yuan, Li, Lei, Yang, Hua, Liu, Xudong, Li, Yaqian, Zhu, Lan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281669/
https://www.ncbi.nlm.nih.gov/pubmed/34266489
http://dx.doi.org/10.1186/s13287-021-02488-2
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author Zhang, Ye
Ma, Yidi
Chen, Juan
Wang, Min
Cao, Yuan
Li, Lei
Yang, Hua
Liu, Xudong
Li, Yaqian
Zhu, Lan
author_facet Zhang, Ye
Ma, Yidi
Chen, Juan
Wang, Min
Cao, Yuan
Li, Lei
Yang, Hua
Liu, Xudong
Li, Yaqian
Zhu, Lan
author_sort Zhang, Ye
collection PubMed
description BACKGROUND: Current surgical therapies for pelvic organ prolapse (POP) do not repair weak vaginal tissue and just provide support; these therapies may trigger severe complications. Stem cell-based regenerative therapy, due to its ability to reconstruct damaged tissue, may be a promising therapeutic strategy for POP. The objective of this study is to evaluate whether mesenchymal stem cell (MSC) therapy can repair weak vaginal tissue in an ovariectomized rhesus macaque model. METHODS: A bilateral ovariectomy model was established in rhesus macaques to induce menopause-related vaginal injury. Ten bilaterally ovariectomized rhesus macaques were divided into two groups (n=5/group): the saline group and the MSC group. Three months after ovariectomy, saline or MSCs were injected in situ into the injured vaginal wall. The vaginal tissue was harvested 12 weeks after injection for histological and biochemical analyses to evaluate changes of extracellular matrix, microvascular density, and smooth muscle in the vaginal tissue. Biomechanical properties of the vaginal tissue were assessed by uniaxial tensile testing. Data analysis was performed with unpaired Student’s t test or Mann-Whitney. RESULTS: Twelve weeks after MSC transplantation, histological and biochemical analyses revealed that the content of collagen I, elastin, and microvascular density in the lamina propria of the vagina increased significantly in the MSC group compared with the saline group. And the fraction of smooth muscle in the muscularis of vagina increased significantly in the MSC group. In addition, MSC transplantation improved the biomechanical properties of the vagina by enhancing the elastic modulus. CONCLUSION: Vaginal MSC transplantation could repair the weak vaginal tissue by promoting extracellular matrix ingrowth, neovascularization, and smooth muscle formation and improve the biomechanical properties of the vagina, providing a new prospective treatment for POP. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-021-02488-2.
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spelling pubmed-82816692021-07-16 Mesenchymal stem cell transplantation for vaginal repair in an ovariectomized rhesus macaque model Zhang, Ye Ma, Yidi Chen, Juan Wang, Min Cao, Yuan Li, Lei Yang, Hua Liu, Xudong Li, Yaqian Zhu, Lan Stem Cell Res Ther Research BACKGROUND: Current surgical therapies for pelvic organ prolapse (POP) do not repair weak vaginal tissue and just provide support; these therapies may trigger severe complications. Stem cell-based regenerative therapy, due to its ability to reconstruct damaged tissue, may be a promising therapeutic strategy for POP. The objective of this study is to evaluate whether mesenchymal stem cell (MSC) therapy can repair weak vaginal tissue in an ovariectomized rhesus macaque model. METHODS: A bilateral ovariectomy model was established in rhesus macaques to induce menopause-related vaginal injury. Ten bilaterally ovariectomized rhesus macaques were divided into two groups (n=5/group): the saline group and the MSC group. Three months after ovariectomy, saline or MSCs were injected in situ into the injured vaginal wall. The vaginal tissue was harvested 12 weeks after injection for histological and biochemical analyses to evaluate changes of extracellular matrix, microvascular density, and smooth muscle in the vaginal tissue. Biomechanical properties of the vaginal tissue were assessed by uniaxial tensile testing. Data analysis was performed with unpaired Student’s t test or Mann-Whitney. RESULTS: Twelve weeks after MSC transplantation, histological and biochemical analyses revealed that the content of collagen I, elastin, and microvascular density in the lamina propria of the vagina increased significantly in the MSC group compared with the saline group. And the fraction of smooth muscle in the muscularis of vagina increased significantly in the MSC group. In addition, MSC transplantation improved the biomechanical properties of the vagina by enhancing the elastic modulus. CONCLUSION: Vaginal MSC transplantation could repair the weak vaginal tissue by promoting extracellular matrix ingrowth, neovascularization, and smooth muscle formation and improve the biomechanical properties of the vagina, providing a new prospective treatment for POP. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-021-02488-2. BioMed Central 2021-07-15 /pmc/articles/PMC8281669/ /pubmed/34266489 http://dx.doi.org/10.1186/s13287-021-02488-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhang, Ye
Ma, Yidi
Chen, Juan
Wang, Min
Cao, Yuan
Li, Lei
Yang, Hua
Liu, Xudong
Li, Yaqian
Zhu, Lan
Mesenchymal stem cell transplantation for vaginal repair in an ovariectomized rhesus macaque model
title Mesenchymal stem cell transplantation for vaginal repair in an ovariectomized rhesus macaque model
title_full Mesenchymal stem cell transplantation for vaginal repair in an ovariectomized rhesus macaque model
title_fullStr Mesenchymal stem cell transplantation for vaginal repair in an ovariectomized rhesus macaque model
title_full_unstemmed Mesenchymal stem cell transplantation for vaginal repair in an ovariectomized rhesus macaque model
title_short Mesenchymal stem cell transplantation for vaginal repair in an ovariectomized rhesus macaque model
title_sort mesenchymal stem cell transplantation for vaginal repair in an ovariectomized rhesus macaque model
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281669/
https://www.ncbi.nlm.nih.gov/pubmed/34266489
http://dx.doi.org/10.1186/s13287-021-02488-2
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