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Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas
GRF genes have been confirmed to have important regulatory functions in plant growth, development and response to abiotic stress. Although the genome of Jatropha curcas is sequenced, knowledge about the identification of the species’ GRF genes and their expression patterns is still lacking. In this...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282010/ https://www.ncbi.nlm.nih.gov/pubmed/34265005 http://dx.doi.org/10.1371/journal.pone.0254711 |
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author | Tang, Yuehui Cheng, Wei Li, Shen Li, Ying Wang, Xiang Xie, Jiatong He, Yingying Wang, Yaoyu Niu, Yiru Bao, Xinxin Wu, Qian |
author_facet | Tang, Yuehui Cheng, Wei Li, Shen Li, Ying Wang, Xiang Xie, Jiatong He, Yingying Wang, Yaoyu Niu, Yiru Bao, Xinxin Wu, Qian |
author_sort | Tang, Yuehui |
collection | PubMed |
description | GRF genes have been confirmed to have important regulatory functions in plant growth, development and response to abiotic stress. Although the genome of Jatropha curcas is sequenced, knowledge about the identification of the species’ GRF genes and their expression patterns is still lacking. In this study, we characterized the 10 JcGRF genes. A detailed investigation into the physic nut GRF gene family is performed, including analysis of the exon-intron structure, conserved domains, conserved motifs, phylogeny, chromosomal locations, potential small RNA targets and expression profiles under both normal growth and abiotic stress conditions. Phylogenetic analysis indicated that the 10 JcGRF genes were classified into five groups corresponding to group I, II, III, IV and V. The analysis of conserved domains showed that the motifs of JcGRF genes were highly conserved in Jatropha curcas. Expression analysis based on RNA-seq and qRT-PCR showed that almost all JcGRF genes had the highest expression in seeds, but very low expression was detected in the non-seed tissues tested, and four JcGRF genes responded to at least one abiotic stress at at least one treatment point. Our research will provide an important scientific basis for further research on the potential functions of JcGRF genes in Jatropha curcas growth and development, and response to abiotic stress, and will eventually provide candidate genes for the breeding of Jatropha curcas. |
format | Online Article Text |
id | pubmed-8282010 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-82820102021-07-28 Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas Tang, Yuehui Cheng, Wei Li, Shen Li, Ying Wang, Xiang Xie, Jiatong He, Yingying Wang, Yaoyu Niu, Yiru Bao, Xinxin Wu, Qian PLoS One Research Article GRF genes have been confirmed to have important regulatory functions in plant growth, development and response to abiotic stress. Although the genome of Jatropha curcas is sequenced, knowledge about the identification of the species’ GRF genes and their expression patterns is still lacking. In this study, we characterized the 10 JcGRF genes. A detailed investigation into the physic nut GRF gene family is performed, including analysis of the exon-intron structure, conserved domains, conserved motifs, phylogeny, chromosomal locations, potential small RNA targets and expression profiles under both normal growth and abiotic stress conditions. Phylogenetic analysis indicated that the 10 JcGRF genes were classified into five groups corresponding to group I, II, III, IV and V. The analysis of conserved domains showed that the motifs of JcGRF genes were highly conserved in Jatropha curcas. Expression analysis based on RNA-seq and qRT-PCR showed that almost all JcGRF genes had the highest expression in seeds, but very low expression was detected in the non-seed tissues tested, and four JcGRF genes responded to at least one abiotic stress at at least one treatment point. Our research will provide an important scientific basis for further research on the potential functions of JcGRF genes in Jatropha curcas growth and development, and response to abiotic stress, and will eventually provide candidate genes for the breeding of Jatropha curcas. Public Library of Science 2021-07-15 /pmc/articles/PMC8282010/ /pubmed/34265005 http://dx.doi.org/10.1371/journal.pone.0254711 Text en © 2021 Tang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Tang, Yuehui Cheng, Wei Li, Shen Li, Ying Wang, Xiang Xie, Jiatong He, Yingying Wang, Yaoyu Niu, Yiru Bao, Xinxin Wu, Qian Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas |
title | Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas |
title_full | Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas |
title_fullStr | Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas |
title_full_unstemmed | Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas |
title_short | Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas |
title_sort | genome-wide identification and expression analysis of the growth regulating factor (grf) family in jatropha curcas |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282010/ https://www.ncbi.nlm.nih.gov/pubmed/34265005 http://dx.doi.org/10.1371/journal.pone.0254711 |
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