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Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas

GRF genes have been confirmed to have important regulatory functions in plant growth, development and response to abiotic stress. Although the genome of Jatropha curcas is sequenced, knowledge about the identification of the species’ GRF genes and their expression patterns is still lacking. In this...

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Autores principales: Tang, Yuehui, Cheng, Wei, Li, Shen, Li, Ying, Wang, Xiang, Xie, Jiatong, He, Yingying, Wang, Yaoyu, Niu, Yiru, Bao, Xinxin, Wu, Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282010/
https://www.ncbi.nlm.nih.gov/pubmed/34265005
http://dx.doi.org/10.1371/journal.pone.0254711
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author Tang, Yuehui
Cheng, Wei
Li, Shen
Li, Ying
Wang, Xiang
Xie, Jiatong
He, Yingying
Wang, Yaoyu
Niu, Yiru
Bao, Xinxin
Wu, Qian
author_facet Tang, Yuehui
Cheng, Wei
Li, Shen
Li, Ying
Wang, Xiang
Xie, Jiatong
He, Yingying
Wang, Yaoyu
Niu, Yiru
Bao, Xinxin
Wu, Qian
author_sort Tang, Yuehui
collection PubMed
description GRF genes have been confirmed to have important regulatory functions in plant growth, development and response to abiotic stress. Although the genome of Jatropha curcas is sequenced, knowledge about the identification of the species’ GRF genes and their expression patterns is still lacking. In this study, we characterized the 10 JcGRF genes. A detailed investigation into the physic nut GRF gene family is performed, including analysis of the exon-intron structure, conserved domains, conserved motifs, phylogeny, chromosomal locations, potential small RNA targets and expression profiles under both normal growth and abiotic stress conditions. Phylogenetic analysis indicated that the 10 JcGRF genes were classified into five groups corresponding to group I, II, III, IV and V. The analysis of conserved domains showed that the motifs of JcGRF genes were highly conserved in Jatropha curcas. Expression analysis based on RNA-seq and qRT-PCR showed that almost all JcGRF genes had the highest expression in seeds, but very low expression was detected in the non-seed tissues tested, and four JcGRF genes responded to at least one abiotic stress at at least one treatment point. Our research will provide an important scientific basis for further research on the potential functions of JcGRF genes in Jatropha curcas growth and development, and response to abiotic stress, and will eventually provide candidate genes for the breeding of Jatropha curcas.
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spelling pubmed-82820102021-07-28 Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas Tang, Yuehui Cheng, Wei Li, Shen Li, Ying Wang, Xiang Xie, Jiatong He, Yingying Wang, Yaoyu Niu, Yiru Bao, Xinxin Wu, Qian PLoS One Research Article GRF genes have been confirmed to have important regulatory functions in plant growth, development and response to abiotic stress. Although the genome of Jatropha curcas is sequenced, knowledge about the identification of the species’ GRF genes and their expression patterns is still lacking. In this study, we characterized the 10 JcGRF genes. A detailed investigation into the physic nut GRF gene family is performed, including analysis of the exon-intron structure, conserved domains, conserved motifs, phylogeny, chromosomal locations, potential small RNA targets and expression profiles under both normal growth and abiotic stress conditions. Phylogenetic analysis indicated that the 10 JcGRF genes were classified into five groups corresponding to group I, II, III, IV and V. The analysis of conserved domains showed that the motifs of JcGRF genes were highly conserved in Jatropha curcas. Expression analysis based on RNA-seq and qRT-PCR showed that almost all JcGRF genes had the highest expression in seeds, but very low expression was detected in the non-seed tissues tested, and four JcGRF genes responded to at least one abiotic stress at at least one treatment point. Our research will provide an important scientific basis for further research on the potential functions of JcGRF genes in Jatropha curcas growth and development, and response to abiotic stress, and will eventually provide candidate genes for the breeding of Jatropha curcas. Public Library of Science 2021-07-15 /pmc/articles/PMC8282010/ /pubmed/34265005 http://dx.doi.org/10.1371/journal.pone.0254711 Text en © 2021 Tang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Tang, Yuehui
Cheng, Wei
Li, Shen
Li, Ying
Wang, Xiang
Xie, Jiatong
He, Yingying
Wang, Yaoyu
Niu, Yiru
Bao, Xinxin
Wu, Qian
Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas
title Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas
title_full Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas
title_fullStr Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas
title_full_unstemmed Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas
title_short Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas
title_sort genome-wide identification and expression analysis of the growth regulating factor (grf) family in jatropha curcas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282010/
https://www.ncbi.nlm.nih.gov/pubmed/34265005
http://dx.doi.org/10.1371/journal.pone.0254711
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