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The epidemic of Q fever in 2018 to 2019 in Zhuhai city of China determined by metagenomic next-generation sequencing
Q fever is a worldwide zoonosis caused by Coxiella burnetii (Cb). From January 2018 to November 2019, plasma samples from 2,382 patients with acute fever of unknown cause at a hospital in Zhuhai city of China were tested using metagenomic next-generation sequencing (mNGS). Of those tested, 138 patie...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282036/ https://www.ncbi.nlm.nih.gov/pubmed/34264939 http://dx.doi.org/10.1371/journal.pntd.0009520 |
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author | Huang, Mingxing Ma, Jinmin Jiao, Jun Li, Chunna Chen, Luan Zhu, Zhongyi Ruan, Feng Xing, Li Zheng, Xinchun Fu, Mengjiao Ma, Binyin Gan, Chongjie Mao, Yuanchen Zhang, Chongnan Sun, Ping Liu, Xi Lin, Ziliang Chen, Lu Lu, Zhiyu Zhou, Dongsheng Wen, Bohai Chen, Weijun Xiong, Xiaolu Xia, Jinyu |
author_facet | Huang, Mingxing Ma, Jinmin Jiao, Jun Li, Chunna Chen, Luan Zhu, Zhongyi Ruan, Feng Xing, Li Zheng, Xinchun Fu, Mengjiao Ma, Binyin Gan, Chongjie Mao, Yuanchen Zhang, Chongnan Sun, Ping Liu, Xi Lin, Ziliang Chen, Lu Lu, Zhiyu Zhou, Dongsheng Wen, Bohai Chen, Weijun Xiong, Xiaolu Xia, Jinyu |
author_sort | Huang, Mingxing |
collection | PubMed |
description | Q fever is a worldwide zoonosis caused by Coxiella burnetii (Cb). From January 2018 to November 2019, plasma samples from 2,382 patients with acute fever of unknown cause at a hospital in Zhuhai city of China were tested using metagenomic next-generation sequencing (mNGS). Of those tested, 138 patients (5.8%) were diagnosed with Q fever based on the presence of Cb genomic DNA detected by mNGS. Among these, 78 cases (56.5%) presented from Nov 2018 to Mar 2019, suggesting an outbreak of Q fever. 55 cases with detailed clinical information that occurred during the outbreak period were used for further analysis. The vast majority of plasma samples from those Cb-mNGS-positive patients were positive in a Cb-specific quantitative polymerase chain reaction (n = 38) and/or indirect immunofluorescence assay (n = 26). Mobile phone tracing data was used to define the area of infection during the outbreak. This suggested the probable infection source was Cb-infected goats and cattle at the only official authorized slaughterhouse in Zhuhai city. Phylogenic analysis based on genomic sequences indicated Cb strains identified in the patients, goat and cattle were formed a single branch, most closely related to the genomic group of Cb dominated by strains isolated from goats. Our study demonstrates Q fever was epidemic in 2018–2019 in Zhuhai city, and this is the first confirmed epidemic of Q fever in a contemporary city in China. |
format | Online Article Text |
id | pubmed-8282036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-82820362021-07-28 The epidemic of Q fever in 2018 to 2019 in Zhuhai city of China determined by metagenomic next-generation sequencing Huang, Mingxing Ma, Jinmin Jiao, Jun Li, Chunna Chen, Luan Zhu, Zhongyi Ruan, Feng Xing, Li Zheng, Xinchun Fu, Mengjiao Ma, Binyin Gan, Chongjie Mao, Yuanchen Zhang, Chongnan Sun, Ping Liu, Xi Lin, Ziliang Chen, Lu Lu, Zhiyu Zhou, Dongsheng Wen, Bohai Chen, Weijun Xiong, Xiaolu Xia, Jinyu PLoS Negl Trop Dis Research Article Q fever is a worldwide zoonosis caused by Coxiella burnetii (Cb). From January 2018 to November 2019, plasma samples from 2,382 patients with acute fever of unknown cause at a hospital in Zhuhai city of China were tested using metagenomic next-generation sequencing (mNGS). Of those tested, 138 patients (5.8%) were diagnosed with Q fever based on the presence of Cb genomic DNA detected by mNGS. Among these, 78 cases (56.5%) presented from Nov 2018 to Mar 2019, suggesting an outbreak of Q fever. 55 cases with detailed clinical information that occurred during the outbreak period were used for further analysis. The vast majority of plasma samples from those Cb-mNGS-positive patients were positive in a Cb-specific quantitative polymerase chain reaction (n = 38) and/or indirect immunofluorescence assay (n = 26). Mobile phone tracing data was used to define the area of infection during the outbreak. This suggested the probable infection source was Cb-infected goats and cattle at the only official authorized slaughterhouse in Zhuhai city. Phylogenic analysis based on genomic sequences indicated Cb strains identified in the patients, goat and cattle were formed a single branch, most closely related to the genomic group of Cb dominated by strains isolated from goats. Our study demonstrates Q fever was epidemic in 2018–2019 in Zhuhai city, and this is the first confirmed epidemic of Q fever in a contemporary city in China. Public Library of Science 2021-07-15 /pmc/articles/PMC8282036/ /pubmed/34264939 http://dx.doi.org/10.1371/journal.pntd.0009520 Text en © 2021 Huang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Huang, Mingxing Ma, Jinmin Jiao, Jun Li, Chunna Chen, Luan Zhu, Zhongyi Ruan, Feng Xing, Li Zheng, Xinchun Fu, Mengjiao Ma, Binyin Gan, Chongjie Mao, Yuanchen Zhang, Chongnan Sun, Ping Liu, Xi Lin, Ziliang Chen, Lu Lu, Zhiyu Zhou, Dongsheng Wen, Bohai Chen, Weijun Xiong, Xiaolu Xia, Jinyu The epidemic of Q fever in 2018 to 2019 in Zhuhai city of China determined by metagenomic next-generation sequencing |
title | The epidemic of Q fever in 2018 to 2019 in Zhuhai city of China determined by metagenomic next-generation sequencing |
title_full | The epidemic of Q fever in 2018 to 2019 in Zhuhai city of China determined by metagenomic next-generation sequencing |
title_fullStr | The epidemic of Q fever in 2018 to 2019 in Zhuhai city of China determined by metagenomic next-generation sequencing |
title_full_unstemmed | The epidemic of Q fever in 2018 to 2019 in Zhuhai city of China determined by metagenomic next-generation sequencing |
title_short | The epidemic of Q fever in 2018 to 2019 in Zhuhai city of China determined by metagenomic next-generation sequencing |
title_sort | epidemic of q fever in 2018 to 2019 in zhuhai city of china determined by metagenomic next-generation sequencing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282036/ https://www.ncbi.nlm.nih.gov/pubmed/34264939 http://dx.doi.org/10.1371/journal.pntd.0009520 |
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