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Pak1 and PP2A antagonize aPKC function to support cortical tension induced by the Crumbs-Yurt complex

The Drosophila polarity protein Crumbs is essential for the establishment and growth of the apical domain in epithelial cells. The protein Yurt limits the ability of Crumbs to promote apical membrane growth, thereby defining proper apical/lateral membrane ratio that is crucial for forming and mainta...

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Autores principales: Biehler, Cornelia, Rothenberg, Katheryn E, Jette, Alexandra, Gaude, Helori-Mael, Fernandez-Gonzalez, Rodrigo, Laprise, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282337/
https://www.ncbi.nlm.nih.gov/pubmed/34212861
http://dx.doi.org/10.7554/eLife.67999
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author Biehler, Cornelia
Rothenberg, Katheryn E
Jette, Alexandra
Gaude, Helori-Mael
Fernandez-Gonzalez, Rodrigo
Laprise, Patrick
author_facet Biehler, Cornelia
Rothenberg, Katheryn E
Jette, Alexandra
Gaude, Helori-Mael
Fernandez-Gonzalez, Rodrigo
Laprise, Patrick
author_sort Biehler, Cornelia
collection PubMed
description The Drosophila polarity protein Crumbs is essential for the establishment and growth of the apical domain in epithelial cells. The protein Yurt limits the ability of Crumbs to promote apical membrane growth, thereby defining proper apical/lateral membrane ratio that is crucial for forming and maintaining complex epithelial structures such as tubes or acini. Here, we show that Yurt also increases Myosin-dependent cortical tension downstream of Crumbs. Yurt overexpression thus induces apical constriction in epithelial cells. The kinase aPKC phosphorylates Yurt, thereby dislodging the latter from the apical domain and releasing apical tension. In contrast, the kinase Pak1 promotes Yurt dephosphorylation through activation of the phosphatase PP2A. The Pak1–PP2A module thus opposes aPKC function and supports Yurt-induced apical constriction. Hence, the complex interplay between Yurt, aPKC, Pak1, and PP2A contributes to the functional plasticity of Crumbs. Overall, our data increase our understanding of how proteins sustaining epithelial cell polarization and Myosin-dependent cell contractility interact with one another to control epithelial tissue architecture.
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spelling pubmed-82823372021-07-19 Pak1 and PP2A antagonize aPKC function to support cortical tension induced by the Crumbs-Yurt complex Biehler, Cornelia Rothenberg, Katheryn E Jette, Alexandra Gaude, Helori-Mael Fernandez-Gonzalez, Rodrigo Laprise, Patrick eLife Developmental Biology The Drosophila polarity protein Crumbs is essential for the establishment and growth of the apical domain in epithelial cells. The protein Yurt limits the ability of Crumbs to promote apical membrane growth, thereby defining proper apical/lateral membrane ratio that is crucial for forming and maintaining complex epithelial structures such as tubes or acini. Here, we show that Yurt also increases Myosin-dependent cortical tension downstream of Crumbs. Yurt overexpression thus induces apical constriction in epithelial cells. The kinase aPKC phosphorylates Yurt, thereby dislodging the latter from the apical domain and releasing apical tension. In contrast, the kinase Pak1 promotes Yurt dephosphorylation through activation of the phosphatase PP2A. The Pak1–PP2A module thus opposes aPKC function and supports Yurt-induced apical constriction. Hence, the complex interplay between Yurt, aPKC, Pak1, and PP2A contributes to the functional plasticity of Crumbs. Overall, our data increase our understanding of how proteins sustaining epithelial cell polarization and Myosin-dependent cell contractility interact with one another to control epithelial tissue architecture. eLife Sciences Publications, Ltd 2021-07-02 /pmc/articles/PMC8282337/ /pubmed/34212861 http://dx.doi.org/10.7554/eLife.67999 Text en © 2021, Biehler et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Biehler, Cornelia
Rothenberg, Katheryn E
Jette, Alexandra
Gaude, Helori-Mael
Fernandez-Gonzalez, Rodrigo
Laprise, Patrick
Pak1 and PP2A antagonize aPKC function to support cortical tension induced by the Crumbs-Yurt complex
title Pak1 and PP2A antagonize aPKC function to support cortical tension induced by the Crumbs-Yurt complex
title_full Pak1 and PP2A antagonize aPKC function to support cortical tension induced by the Crumbs-Yurt complex
title_fullStr Pak1 and PP2A antagonize aPKC function to support cortical tension induced by the Crumbs-Yurt complex
title_full_unstemmed Pak1 and PP2A antagonize aPKC function to support cortical tension induced by the Crumbs-Yurt complex
title_short Pak1 and PP2A antagonize aPKC function to support cortical tension induced by the Crumbs-Yurt complex
title_sort pak1 and pp2a antagonize apkc function to support cortical tension induced by the crumbs-yurt complex
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282337/
https://www.ncbi.nlm.nih.gov/pubmed/34212861
http://dx.doi.org/10.7554/eLife.67999
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