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Functional interdependence of the actin nucleator Cobl and Cobl-like in dendritic arbor development
Local actin filament formation is indispensable for development of the dendritic arbor of neurons. We show that, surprisingly, the action of single actin filament-promoting factors was insufficient for powering dendritogenesis. Instead, this required the actin nucleator Cobl and its only evolutionar...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282341/ https://www.ncbi.nlm.nih.gov/pubmed/34264190 http://dx.doi.org/10.7554/eLife.67718 |
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author | Izadi, Maryam Seemann, Eric Schlobinski, Dirk Schwintzer, Lukas Qualmann, Britta Kessels, Michael M |
author_facet | Izadi, Maryam Seemann, Eric Schlobinski, Dirk Schwintzer, Lukas Qualmann, Britta Kessels, Michael M |
author_sort | Izadi, Maryam |
collection | PubMed |
description | Local actin filament formation is indispensable for development of the dendritic arbor of neurons. We show that, surprisingly, the action of single actin filament-promoting factors was insufficient for powering dendritogenesis. Instead, this required the actin nucleator Cobl and its only evolutionary distant ancestor Cobl-like acting interdependently. This coordination between Cobl-like and Cobl was achieved by physical linkage by syndapins. Syndapin I formed nanodomains at convex plasma membrane areas at the base of protrusive structures and interacted with three motifs in Cobl-like, one of which was Ca(2+)/calmodulin-regulated. Consistently, syndapin I, Cobl-like’s newly identified N terminal calmodulin-binding site and the single Ca(2+)/calmodulin-responsive syndapin-binding motif all were critical for Cobl-like’s functions. In dendritic arbor development, local Ca(2+)/CaM-controlled actin dynamics thus relies on regulated and physically coordinated interactions of different F-actin formation-promoting factors and only together they have the power to bring about the sophisticated neuronal morphologies required for neuronal network formation in mammals. |
format | Online Article Text |
id | pubmed-8282341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-82823412021-07-19 Functional interdependence of the actin nucleator Cobl and Cobl-like in dendritic arbor development Izadi, Maryam Seemann, Eric Schlobinski, Dirk Schwintzer, Lukas Qualmann, Britta Kessels, Michael M eLife Cell Biology Local actin filament formation is indispensable for development of the dendritic arbor of neurons. We show that, surprisingly, the action of single actin filament-promoting factors was insufficient for powering dendritogenesis. Instead, this required the actin nucleator Cobl and its only evolutionary distant ancestor Cobl-like acting interdependently. This coordination between Cobl-like and Cobl was achieved by physical linkage by syndapins. Syndapin I formed nanodomains at convex plasma membrane areas at the base of protrusive structures and interacted with three motifs in Cobl-like, one of which was Ca(2+)/calmodulin-regulated. Consistently, syndapin I, Cobl-like’s newly identified N terminal calmodulin-binding site and the single Ca(2+)/calmodulin-responsive syndapin-binding motif all were critical for Cobl-like’s functions. In dendritic arbor development, local Ca(2+)/CaM-controlled actin dynamics thus relies on regulated and physically coordinated interactions of different F-actin formation-promoting factors and only together they have the power to bring about the sophisticated neuronal morphologies required for neuronal network formation in mammals. eLife Sciences Publications, Ltd 2021-07-15 /pmc/articles/PMC8282341/ /pubmed/34264190 http://dx.doi.org/10.7554/eLife.67718 Text en © 2021, Izadi et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Izadi, Maryam Seemann, Eric Schlobinski, Dirk Schwintzer, Lukas Qualmann, Britta Kessels, Michael M Functional interdependence of the actin nucleator Cobl and Cobl-like in dendritic arbor development |
title | Functional interdependence of the actin nucleator Cobl and Cobl-like in dendritic arbor development |
title_full | Functional interdependence of the actin nucleator Cobl and Cobl-like in dendritic arbor development |
title_fullStr | Functional interdependence of the actin nucleator Cobl and Cobl-like in dendritic arbor development |
title_full_unstemmed | Functional interdependence of the actin nucleator Cobl and Cobl-like in dendritic arbor development |
title_short | Functional interdependence of the actin nucleator Cobl and Cobl-like in dendritic arbor development |
title_sort | functional interdependence of the actin nucleator cobl and cobl-like in dendritic arbor development |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282341/ https://www.ncbi.nlm.nih.gov/pubmed/34264190 http://dx.doi.org/10.7554/eLife.67718 |
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