Stokes polarimetry-based second harmonic generation microscopy for collagen and skeletal muscle fiber characterization

The complete polarization state of second harmonic (SH) light was measured and characterized by collagen type I and skeletal muscle fiber using a Stokes vector-based SHG microscope. The polarization states of the SH signal are analyzed in a pixel-by-pixel manner and displayed through two dimensional...

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Detalles Bibliográficos
Autores principales: Mazumder, Nirmal, Kao, Fu-Jen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer London 2020
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282547/
https://www.ncbi.nlm.nih.gov/pubmed/32945997
http://dx.doi.org/10.1007/s10103-020-03144-6
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author Mazumder, Nirmal
Kao, Fu-Jen
author_facet Mazumder, Nirmal
Kao, Fu-Jen
author_sort Mazumder, Nirmal
collection PubMed
description The complete polarization state of second harmonic (SH) light was measured and characterized by collagen type I and skeletal muscle fiber using a Stokes vector-based SHG microscope. The polarization states of the SH signal are analyzed in a pixel-by-pixel manner and displayed through two dimensional (2D) Stokes vector images. Various polarization parameters are reconstructed using Stokes values to quantify the polarization properties of SH light. Also, the measurements are extended for different input polarization states to investigate the molecular structure of second harmonic generation (SHG) active molecules such as collagen type I and myosin.
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spelling pubmed-82825472021-07-20 Stokes polarimetry-based second harmonic generation microscopy for collagen and skeletal muscle fiber characterization Mazumder, Nirmal Kao, Fu-Jen Lasers Med Sci Original Article The complete polarization state of second harmonic (SH) light was measured and characterized by collagen type I and skeletal muscle fiber using a Stokes vector-based SHG microscope. The polarization states of the SH signal are analyzed in a pixel-by-pixel manner and displayed through two dimensional (2D) Stokes vector images. Various polarization parameters are reconstructed using Stokes values to quantify the polarization properties of SH light. Also, the measurements are extended for different input polarization states to investigate the molecular structure of second harmonic generation (SHG) active molecules such as collagen type I and myosin. Springer London 2020-09-18 2021 /pmc/articles/PMC8282547/ /pubmed/32945997 http://dx.doi.org/10.1007/s10103-020-03144-6 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Mazumder, Nirmal
Kao, Fu-Jen
Stokes polarimetry-based second harmonic generation microscopy for collagen and skeletal muscle fiber characterization
title Stokes polarimetry-based second harmonic generation microscopy for collagen and skeletal muscle fiber characterization
title_full Stokes polarimetry-based second harmonic generation microscopy for collagen and skeletal muscle fiber characterization
title_fullStr Stokes polarimetry-based second harmonic generation microscopy for collagen and skeletal muscle fiber characterization
title_full_unstemmed Stokes polarimetry-based second harmonic generation microscopy for collagen and skeletal muscle fiber characterization
title_short Stokes polarimetry-based second harmonic generation microscopy for collagen and skeletal muscle fiber characterization
title_sort stokes polarimetry-based second harmonic generation microscopy for collagen and skeletal muscle fiber characterization
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282547/
https://www.ncbi.nlm.nih.gov/pubmed/32945997
http://dx.doi.org/10.1007/s10103-020-03144-6
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