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Correlation between human health and reactive oxygen species produced in blood: a long-term chemiluminescence and fluorescence analysis

The previous slide-glass type system could simultaneously detect reactive and highly reactive oxygen species, i.e., superoxide radicals (O(2)(−·)) and hypochlorite ions (OCl(−)) elicited from leucocytes in sample blood, but had some drawbacks, i.e., signal noise from air-flow stirring, potential bio...

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Autores principales: Kazumura, Kimiko, Takeuchi, Kozo, Hatano, Yukiko, Hara, Akiko, Miwa, Toshiyuki, Hattori, Masaki, Kondo, Fusanori, Morishita, Naokazu, Tsuchiya, Hiroshi, Osawa, Toshihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282623/
https://www.ncbi.nlm.nih.gov/pubmed/34267248
http://dx.doi.org/10.1038/s41598-021-93887-1
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author Kazumura, Kimiko
Takeuchi, Kozo
Hatano, Yukiko
Hara, Akiko
Miwa, Toshiyuki
Hattori, Masaki
Kondo, Fusanori
Morishita, Naokazu
Tsuchiya, Hiroshi
Osawa, Toshihiko
author_facet Kazumura, Kimiko
Takeuchi, Kozo
Hatano, Yukiko
Hara, Akiko
Miwa, Toshiyuki
Hattori, Masaki
Kondo, Fusanori
Morishita, Naokazu
Tsuchiya, Hiroshi
Osawa, Toshihiko
author_sort Kazumura, Kimiko
collection PubMed
description The previous slide-glass type system could simultaneously detect reactive and highly reactive oxygen species, i.e., superoxide radicals (O(2)(−·)) and hypochlorite ions (OCl(−)) elicited from leucocytes in sample blood, but had some drawbacks, i.e., signal noise from air-flow stirring, potential biohazard risks, etc. because of open samples placed on a slide glass. We overcame these drawbacks by adopting a fluidic-chip container in a new system, which resulted in higher sensitivity and more stable measurements. Using the new system, we conducted a pilot study on nominally healthy volunteers to find whether or not the monitored activities of leukocytes can distinguish more or less unhealthy conditions from healthy ones. At first, healthy volunteers of both genders and of various ages showed that the fluctuation magnitudes (%) of O(2)(−·) and OCl(−) were nearly similar to each other and to that of the neutrophil count fluctuation. These parameters sometimes exceeded the healthy fluctuation range. By comparing these large fluctuations with the data of an inflammation marker C-reactive protein (CRP), the neutrophil count fluctuation and the timings/symptoms of abnormalities found in questionnaire, we could gain information suggesting the factors causing the large fluctuations. The new system could detect bodily abnormalities earlier than CRP or self-aware symptoms.
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spelling pubmed-82826232021-07-19 Correlation between human health and reactive oxygen species produced in blood: a long-term chemiluminescence and fluorescence analysis Kazumura, Kimiko Takeuchi, Kozo Hatano, Yukiko Hara, Akiko Miwa, Toshiyuki Hattori, Masaki Kondo, Fusanori Morishita, Naokazu Tsuchiya, Hiroshi Osawa, Toshihiko Sci Rep Article The previous slide-glass type system could simultaneously detect reactive and highly reactive oxygen species, i.e., superoxide radicals (O(2)(−·)) and hypochlorite ions (OCl(−)) elicited from leucocytes in sample blood, but had some drawbacks, i.e., signal noise from air-flow stirring, potential biohazard risks, etc. because of open samples placed on a slide glass. We overcame these drawbacks by adopting a fluidic-chip container in a new system, which resulted in higher sensitivity and more stable measurements. Using the new system, we conducted a pilot study on nominally healthy volunteers to find whether or not the monitored activities of leukocytes can distinguish more or less unhealthy conditions from healthy ones. At first, healthy volunteers of both genders and of various ages showed that the fluctuation magnitudes (%) of O(2)(−·) and OCl(−) were nearly similar to each other and to that of the neutrophil count fluctuation. These parameters sometimes exceeded the healthy fluctuation range. By comparing these large fluctuations with the data of an inflammation marker C-reactive protein (CRP), the neutrophil count fluctuation and the timings/symptoms of abnormalities found in questionnaire, we could gain information suggesting the factors causing the large fluctuations. The new system could detect bodily abnormalities earlier than CRP or self-aware symptoms. Nature Publishing Group UK 2021-07-15 /pmc/articles/PMC8282623/ /pubmed/34267248 http://dx.doi.org/10.1038/s41598-021-93887-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kazumura, Kimiko
Takeuchi, Kozo
Hatano, Yukiko
Hara, Akiko
Miwa, Toshiyuki
Hattori, Masaki
Kondo, Fusanori
Morishita, Naokazu
Tsuchiya, Hiroshi
Osawa, Toshihiko
Correlation between human health and reactive oxygen species produced in blood: a long-term chemiluminescence and fluorescence analysis
title Correlation between human health and reactive oxygen species produced in blood: a long-term chemiluminescence and fluorescence analysis
title_full Correlation between human health and reactive oxygen species produced in blood: a long-term chemiluminescence and fluorescence analysis
title_fullStr Correlation between human health and reactive oxygen species produced in blood: a long-term chemiluminescence and fluorescence analysis
title_full_unstemmed Correlation between human health and reactive oxygen species produced in blood: a long-term chemiluminescence and fluorescence analysis
title_short Correlation between human health and reactive oxygen species produced in blood: a long-term chemiluminescence and fluorescence analysis
title_sort correlation between human health and reactive oxygen species produced in blood: a long-term chemiluminescence and fluorescence analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282623/
https://www.ncbi.nlm.nih.gov/pubmed/34267248
http://dx.doi.org/10.1038/s41598-021-93887-1
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