Cargando…

Probiotic supplementation reduces inflammatory profiles but does not prevent oral immune perturbations during SIV infection

HIV/SIV infections lead to massive loss of mucosal CD4 + T cells and breakdown of the epithelial mucosa resulting in severe microbial dysbiosis and chronic immune activation that ultimately drive disease progression. Moreover, disruption of one of the most understudied mucosal environments, the oral...

Descripción completa

Detalles Bibliográficos
Autores principales: Jones, Rhianna, Kroll, Kyle, Broedlow, Courtney, Schifanella, Luca, Smith, Scott, Hueber, Brady, Shah, Spandan V., Ram, Daniel R., Manickam, Cordelia, Varner, Valerie, Klatt, Nichole R., Reeves, R. Keith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282626/
https://www.ncbi.nlm.nih.gov/pubmed/34267278
http://dx.doi.org/10.1038/s41598-021-93918-x
_version_ 1783723049669885952
author Jones, Rhianna
Kroll, Kyle
Broedlow, Courtney
Schifanella, Luca
Smith, Scott
Hueber, Brady
Shah, Spandan V.
Ram, Daniel R.
Manickam, Cordelia
Varner, Valerie
Klatt, Nichole R.
Reeves, R. Keith
author_facet Jones, Rhianna
Kroll, Kyle
Broedlow, Courtney
Schifanella, Luca
Smith, Scott
Hueber, Brady
Shah, Spandan V.
Ram, Daniel R.
Manickam, Cordelia
Varner, Valerie
Klatt, Nichole R.
Reeves, R. Keith
author_sort Jones, Rhianna
collection PubMed
description HIV/SIV infections lead to massive loss of mucosal CD4 + T cells and breakdown of the epithelial mucosa resulting in severe microbial dysbiosis and chronic immune activation that ultimately drive disease progression. Moreover, disruption of one of the most understudied mucosal environments, the oral cavity, during HIV-induced immunosuppression results in significant microbial and neoplastic co-morbidities and contributes to and predicts distal disease complications. In this study we evaluated the effects of oral probiotic supplementation (PBX), which can stimulate and augment inflammatory or anti-inflammatory pathways, on early SIV infection of rhesus macaques. Our study revealed that similar to the GI mucosae, oral CD4 + T cells were rapidly depleted, and as one of the first comprehensive analyses of the oral microflora in SIV infection, we also observed significant modulation among two genera, Porphyromonas and Actinobacillus, early after infection. Interestingly, although PBX therapy did not substantially protect against oral dysbiosis or ameliorate cell loss, it did somewhat dampen inflammation and T cell activation. Collectively, these data provide one of the most comprehensive evaluations of SIV-induced changes in oral microbiome and CD4 + T cell populations, and also suggest that oral PBX may have some anti-inflammatory properties in lentivirus infections.
format Online
Article
Text
id pubmed-8282626
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-82826262021-07-19 Probiotic supplementation reduces inflammatory profiles but does not prevent oral immune perturbations during SIV infection Jones, Rhianna Kroll, Kyle Broedlow, Courtney Schifanella, Luca Smith, Scott Hueber, Brady Shah, Spandan V. Ram, Daniel R. Manickam, Cordelia Varner, Valerie Klatt, Nichole R. Reeves, R. Keith Sci Rep Article HIV/SIV infections lead to massive loss of mucosal CD4 + T cells and breakdown of the epithelial mucosa resulting in severe microbial dysbiosis and chronic immune activation that ultimately drive disease progression. Moreover, disruption of one of the most understudied mucosal environments, the oral cavity, during HIV-induced immunosuppression results in significant microbial and neoplastic co-morbidities and contributes to and predicts distal disease complications. In this study we evaluated the effects of oral probiotic supplementation (PBX), which can stimulate and augment inflammatory or anti-inflammatory pathways, on early SIV infection of rhesus macaques. Our study revealed that similar to the GI mucosae, oral CD4 + T cells were rapidly depleted, and as one of the first comprehensive analyses of the oral microflora in SIV infection, we also observed significant modulation among two genera, Porphyromonas and Actinobacillus, early after infection. Interestingly, although PBX therapy did not substantially protect against oral dysbiosis or ameliorate cell loss, it did somewhat dampen inflammation and T cell activation. Collectively, these data provide one of the most comprehensive evaluations of SIV-induced changes in oral microbiome and CD4 + T cell populations, and also suggest that oral PBX may have some anti-inflammatory properties in lentivirus infections. Nature Publishing Group UK 2021-07-15 /pmc/articles/PMC8282626/ /pubmed/34267278 http://dx.doi.org/10.1038/s41598-021-93918-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Jones, Rhianna
Kroll, Kyle
Broedlow, Courtney
Schifanella, Luca
Smith, Scott
Hueber, Brady
Shah, Spandan V.
Ram, Daniel R.
Manickam, Cordelia
Varner, Valerie
Klatt, Nichole R.
Reeves, R. Keith
Probiotic supplementation reduces inflammatory profiles but does not prevent oral immune perturbations during SIV infection
title Probiotic supplementation reduces inflammatory profiles but does not prevent oral immune perturbations during SIV infection
title_full Probiotic supplementation reduces inflammatory profiles but does not prevent oral immune perturbations during SIV infection
title_fullStr Probiotic supplementation reduces inflammatory profiles but does not prevent oral immune perturbations during SIV infection
title_full_unstemmed Probiotic supplementation reduces inflammatory profiles but does not prevent oral immune perturbations during SIV infection
title_short Probiotic supplementation reduces inflammatory profiles but does not prevent oral immune perturbations during SIV infection
title_sort probiotic supplementation reduces inflammatory profiles but does not prevent oral immune perturbations during siv infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282626/
https://www.ncbi.nlm.nih.gov/pubmed/34267278
http://dx.doi.org/10.1038/s41598-021-93918-x
work_keys_str_mv AT jonesrhianna probioticsupplementationreducesinflammatoryprofilesbutdoesnotpreventoralimmuneperturbationsduringsivinfection
AT krollkyle probioticsupplementationreducesinflammatoryprofilesbutdoesnotpreventoralimmuneperturbationsduringsivinfection
AT broedlowcourtney probioticsupplementationreducesinflammatoryprofilesbutdoesnotpreventoralimmuneperturbationsduringsivinfection
AT schifanellaluca probioticsupplementationreducesinflammatoryprofilesbutdoesnotpreventoralimmuneperturbationsduringsivinfection
AT smithscott probioticsupplementationreducesinflammatoryprofilesbutdoesnotpreventoralimmuneperturbationsduringsivinfection
AT hueberbrady probioticsupplementationreducesinflammatoryprofilesbutdoesnotpreventoralimmuneperturbationsduringsivinfection
AT shahspandanv probioticsupplementationreducesinflammatoryprofilesbutdoesnotpreventoralimmuneperturbationsduringsivinfection
AT ramdanielr probioticsupplementationreducesinflammatoryprofilesbutdoesnotpreventoralimmuneperturbationsduringsivinfection
AT manickamcordelia probioticsupplementationreducesinflammatoryprofilesbutdoesnotpreventoralimmuneperturbationsduringsivinfection
AT varnervalerie probioticsupplementationreducesinflammatoryprofilesbutdoesnotpreventoralimmuneperturbationsduringsivinfection
AT klattnicholer probioticsupplementationreducesinflammatoryprofilesbutdoesnotpreventoralimmuneperturbationsduringsivinfection
AT reevesrkeith probioticsupplementationreducesinflammatoryprofilesbutdoesnotpreventoralimmuneperturbationsduringsivinfection