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Mechanism for enhancing the growth of mung bean seedlings under simulated microgravity

To elucidate a mechanism for enhancing mung bean seedlings’ growth under microgravity conditions, we measured growth, gene expression, and enzyme activity under clinorotation (20 rpm), and compared data obtained to those grown under normal gravity conditions (control). An increase in fresh weight, w...

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Detalles Bibliográficos
Autores principales: Nakajima, Shusaku, Nagata, Masayasu, Ikehata, Akifumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282859/
https://www.ncbi.nlm.nih.gov/pubmed/34267213
http://dx.doi.org/10.1038/s41526-021-00156-6
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author Nakajima, Shusaku
Nagata, Masayasu
Ikehata, Akifumi
author_facet Nakajima, Shusaku
Nagata, Masayasu
Ikehata, Akifumi
author_sort Nakajima, Shusaku
collection PubMed
description To elucidate a mechanism for enhancing mung bean seedlings’ growth under microgravity conditions, we measured growth, gene expression, and enzyme activity under clinorotation (20 rpm), and compared data obtained to those grown under normal gravity conditions (control). An increase in fresh weight, water content, and lengths were observed in the clinostat seedlings, compared to those of the control seedlings. Real-time PCR showed that aquaporin expression and the amylase gene were upregulated under clinorotation. Additionally, seedlings under clinorotation exhibited a significantly higher amylase activity. Near-infrared image showed that there was no restriction of water evaporation from the seedlings under clinorotation. Therefore, these results indicate that simulated microgravity could induce water uptake, resulting in enhanced amylase activity and seedling growth. Upregulated aquaporin expression could be the first trigger for enhanced growth under clinorotation. We speculated that the seedlings under clinorotation do not use energy against gravitational force and consumed surplus energy for enhanced growth.
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spelling pubmed-82828592021-07-23 Mechanism for enhancing the growth of mung bean seedlings under simulated microgravity Nakajima, Shusaku Nagata, Masayasu Ikehata, Akifumi NPJ Microgravity Article To elucidate a mechanism for enhancing mung bean seedlings’ growth under microgravity conditions, we measured growth, gene expression, and enzyme activity under clinorotation (20 rpm), and compared data obtained to those grown under normal gravity conditions (control). An increase in fresh weight, water content, and lengths were observed in the clinostat seedlings, compared to those of the control seedlings. Real-time PCR showed that aquaporin expression and the amylase gene were upregulated under clinorotation. Additionally, seedlings under clinorotation exhibited a significantly higher amylase activity. Near-infrared image showed that there was no restriction of water evaporation from the seedlings under clinorotation. Therefore, these results indicate that simulated microgravity could induce water uptake, resulting in enhanced amylase activity and seedling growth. Upregulated aquaporin expression could be the first trigger for enhanced growth under clinorotation. We speculated that the seedlings under clinorotation do not use energy against gravitational force and consumed surplus energy for enhanced growth. Nature Publishing Group UK 2021-07-15 /pmc/articles/PMC8282859/ /pubmed/34267213 http://dx.doi.org/10.1038/s41526-021-00156-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Nakajima, Shusaku
Nagata, Masayasu
Ikehata, Akifumi
Mechanism for enhancing the growth of mung bean seedlings under simulated microgravity
title Mechanism for enhancing the growth of mung bean seedlings under simulated microgravity
title_full Mechanism for enhancing the growth of mung bean seedlings under simulated microgravity
title_fullStr Mechanism for enhancing the growth of mung bean seedlings under simulated microgravity
title_full_unstemmed Mechanism for enhancing the growth of mung bean seedlings under simulated microgravity
title_short Mechanism for enhancing the growth of mung bean seedlings under simulated microgravity
title_sort mechanism for enhancing the growth of mung bean seedlings under simulated microgravity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282859/
https://www.ncbi.nlm.nih.gov/pubmed/34267213
http://dx.doi.org/10.1038/s41526-021-00156-6
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