Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza

The immunogenicity and protective efficacy of vaccines can be enhanced by the selective delivery of antigens to the antigen-presenting cells (APCs). In this study, H9N2 avian influenza virus haemagglutinin (HA) antigen, was targeted by fusing it to single-chain fragment variable (scFv) antibodies sp...

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Autores principales: Shrestha, Angita, Sadeyen, Jean-Remy, Lukosaityte, Deimante, Chang, Pengxiang, Smith, Adrian, Van Hulten, Marielle, Iqbal, Munir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282863/
https://www.ncbi.nlm.nih.gov/pubmed/34267228
http://dx.doi.org/10.1038/s41541-021-00350-3
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author Shrestha, Angita
Sadeyen, Jean-Remy
Lukosaityte, Deimante
Chang, Pengxiang
Smith, Adrian
Van Hulten, Marielle
Iqbal, Munir
author_facet Shrestha, Angita
Sadeyen, Jean-Remy
Lukosaityte, Deimante
Chang, Pengxiang
Smith, Adrian
Van Hulten, Marielle
Iqbal, Munir
author_sort Shrestha, Angita
collection PubMed
description The immunogenicity and protective efficacy of vaccines can be enhanced by the selective delivery of antigens to the antigen-presenting cells (APCs). In this study, H9N2 avian influenza virus haemagglutinin (HA) antigen, was targeted by fusing it to single-chain fragment variable (scFv) antibodies specific to CD83 receptor expressed on chicken APCs. We observed an increased level of IFNγ, IL6, IL1β, IL4, and CxCLi2 mRNA upon stimulation of chicken splenocytes ex vivo by CD83 scFv targeted H9HA. In addition, CD83 scFv targeted H9HA induced higher serum haemagglutinin inhibition activity and virus neutralising antibodies compared to untargeted H9HA, with induction of antibodies as early as day 6 post primary vaccination. Furthermore, chickens vaccinated with CD83 scFv targeted H9HA showed reduced H9N2 challenge virus shedding compared to untargeted H9HA. These results suggest that targeting antigens to CD83 receptors could improve the efficacy of poultry vaccines.
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spelling pubmed-82828632021-07-23 Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza Shrestha, Angita Sadeyen, Jean-Remy Lukosaityte, Deimante Chang, Pengxiang Smith, Adrian Van Hulten, Marielle Iqbal, Munir NPJ Vaccines Article The immunogenicity and protective efficacy of vaccines can be enhanced by the selective delivery of antigens to the antigen-presenting cells (APCs). In this study, H9N2 avian influenza virus haemagglutinin (HA) antigen, was targeted by fusing it to single-chain fragment variable (scFv) antibodies specific to CD83 receptor expressed on chicken APCs. We observed an increased level of IFNγ, IL6, IL1β, IL4, and CxCLi2 mRNA upon stimulation of chicken splenocytes ex vivo by CD83 scFv targeted H9HA. In addition, CD83 scFv targeted H9HA induced higher serum haemagglutinin inhibition activity and virus neutralising antibodies compared to untargeted H9HA, with induction of antibodies as early as day 6 post primary vaccination. Furthermore, chickens vaccinated with CD83 scFv targeted H9HA showed reduced H9N2 challenge virus shedding compared to untargeted H9HA. These results suggest that targeting antigens to CD83 receptors could improve the efficacy of poultry vaccines. Nature Publishing Group UK 2021-07-15 /pmc/articles/PMC8282863/ /pubmed/34267228 http://dx.doi.org/10.1038/s41541-021-00350-3 Text en © The Author(s) 2021, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Shrestha, Angita
Sadeyen, Jean-Remy
Lukosaityte, Deimante
Chang, Pengxiang
Smith, Adrian
Van Hulten, Marielle
Iqbal, Munir
Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza
title Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza
title_full Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza
title_fullStr Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza
title_full_unstemmed Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza
title_short Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza
title_sort selectively targeting haemagglutinin antigen to chicken cd83 receptor induces faster and stronger immunity against avian influenza
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282863/
https://www.ncbi.nlm.nih.gov/pubmed/34267228
http://dx.doi.org/10.1038/s41541-021-00350-3
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