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Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer

Small nuclear RNA is a class of non-coding RNA that widely exist in the nucleus of eukaryotes. Accumulated evidences have shown that small nuclear RNAs are associated with the regulation of gene expression in various tumor types. To explore the gene expression changes and its potential effects media...

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Autores principales: Wang, Zhenxing, Wang, Xi, Wang, Yaobang, Tang, Shaomei, Feng, Chao, Pan, Lixin, Lu, Qinchen, Tao, Yuting, Xie, Yuanliang, Wang, Qiuyan, Tang, Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283811/
https://www.ncbi.nlm.nih.gov/pubmed/34276798
http://dx.doi.org/10.3389/fgene.2021.695597
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author Wang, Zhenxing
Wang, Xi
Wang, Yaobang
Tang, Shaomei
Feng, Chao
Pan, Lixin
Lu, Qinchen
Tao, Yuting
Xie, Yuanliang
Wang, Qiuyan
Tang, Zhong
author_facet Wang, Zhenxing
Wang, Xi
Wang, Yaobang
Tang, Shaomei
Feng, Chao
Pan, Lixin
Lu, Qinchen
Tao, Yuting
Xie, Yuanliang
Wang, Qiuyan
Tang, Zhong
author_sort Wang, Zhenxing
collection PubMed
description Small nuclear RNA is a class of non-coding RNA that widely exist in the nucleus of eukaryotes. Accumulated evidences have shown that small nuclear RNAs are associated with the regulation of gene expression in various tumor types. To explore the gene expression changes and its potential effects mediated by U11 snRNA in bladder cancer cells, U11 snRNA knockout and overexpressed cell lines were constructed and further used to analyze the gene expression changes by RNA sequencing. The differentially expressed genes were found to be mainly enriched in tumor-related pathways both in the U11 knockout and overexpression cell lines, such as NF-kappa B signaling pathway, bladder cancer and PI3K-Akt signaling pathway. Furthermore, alternative splicing events were proposed to participate in the potential regulatory mechanism induced by the U11 knockout or overexpression. In conclusion, U11 may be involved in the regulation of gene expression in bladder cancer cells, which may provide a potentially new biomarker for clinical diagnosis and treatment of bladder cancer.
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spelling pubmed-82838112021-07-17 Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer Wang, Zhenxing Wang, Xi Wang, Yaobang Tang, Shaomei Feng, Chao Pan, Lixin Lu, Qinchen Tao, Yuting Xie, Yuanliang Wang, Qiuyan Tang, Zhong Front Genet Genetics Small nuclear RNA is a class of non-coding RNA that widely exist in the nucleus of eukaryotes. Accumulated evidences have shown that small nuclear RNAs are associated with the regulation of gene expression in various tumor types. To explore the gene expression changes and its potential effects mediated by U11 snRNA in bladder cancer cells, U11 snRNA knockout and overexpressed cell lines were constructed and further used to analyze the gene expression changes by RNA sequencing. The differentially expressed genes were found to be mainly enriched in tumor-related pathways both in the U11 knockout and overexpression cell lines, such as NF-kappa B signaling pathway, bladder cancer and PI3K-Akt signaling pathway. Furthermore, alternative splicing events were proposed to participate in the potential regulatory mechanism induced by the U11 knockout or overexpression. In conclusion, U11 may be involved in the regulation of gene expression in bladder cancer cells, which may provide a potentially new biomarker for clinical diagnosis and treatment of bladder cancer. Frontiers Media S.A. 2021-07-02 /pmc/articles/PMC8283811/ /pubmed/34276798 http://dx.doi.org/10.3389/fgene.2021.695597 Text en Copyright © 2021 Wang, Wang, Wang, Tang, Feng, Pan, Lu, Tao, Xie, Wang and Tang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Wang, Zhenxing
Wang, Xi
Wang, Yaobang
Tang, Shaomei
Feng, Chao
Pan, Lixin
Lu, Qinchen
Tao, Yuting
Xie, Yuanliang
Wang, Qiuyan
Tang, Zhong
Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer
title Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer
title_full Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer
title_fullStr Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer
title_full_unstemmed Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer
title_short Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer
title_sort transcriptomic analysis of gene networks regulated by u11 small nuclear rna in bladder cancer
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283811/
https://www.ncbi.nlm.nih.gov/pubmed/34276798
http://dx.doi.org/10.3389/fgene.2021.695597
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