Cargando…
Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer
Small nuclear RNA is a class of non-coding RNA that widely exist in the nucleus of eukaryotes. Accumulated evidences have shown that small nuclear RNAs are associated with the regulation of gene expression in various tumor types. To explore the gene expression changes and its potential effects media...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283811/ https://www.ncbi.nlm.nih.gov/pubmed/34276798 http://dx.doi.org/10.3389/fgene.2021.695597 |
_version_ | 1783723276438077440 |
---|---|
author | Wang, Zhenxing Wang, Xi Wang, Yaobang Tang, Shaomei Feng, Chao Pan, Lixin Lu, Qinchen Tao, Yuting Xie, Yuanliang Wang, Qiuyan Tang, Zhong |
author_facet | Wang, Zhenxing Wang, Xi Wang, Yaobang Tang, Shaomei Feng, Chao Pan, Lixin Lu, Qinchen Tao, Yuting Xie, Yuanliang Wang, Qiuyan Tang, Zhong |
author_sort | Wang, Zhenxing |
collection | PubMed |
description | Small nuclear RNA is a class of non-coding RNA that widely exist in the nucleus of eukaryotes. Accumulated evidences have shown that small nuclear RNAs are associated with the regulation of gene expression in various tumor types. To explore the gene expression changes and its potential effects mediated by U11 snRNA in bladder cancer cells, U11 snRNA knockout and overexpressed cell lines were constructed and further used to analyze the gene expression changes by RNA sequencing. The differentially expressed genes were found to be mainly enriched in tumor-related pathways both in the U11 knockout and overexpression cell lines, such as NF-kappa B signaling pathway, bladder cancer and PI3K-Akt signaling pathway. Furthermore, alternative splicing events were proposed to participate in the potential regulatory mechanism induced by the U11 knockout or overexpression. In conclusion, U11 may be involved in the regulation of gene expression in bladder cancer cells, which may provide a potentially new biomarker for clinical diagnosis and treatment of bladder cancer. |
format | Online Article Text |
id | pubmed-8283811 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82838112021-07-17 Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer Wang, Zhenxing Wang, Xi Wang, Yaobang Tang, Shaomei Feng, Chao Pan, Lixin Lu, Qinchen Tao, Yuting Xie, Yuanliang Wang, Qiuyan Tang, Zhong Front Genet Genetics Small nuclear RNA is a class of non-coding RNA that widely exist in the nucleus of eukaryotes. Accumulated evidences have shown that small nuclear RNAs are associated with the regulation of gene expression in various tumor types. To explore the gene expression changes and its potential effects mediated by U11 snRNA in bladder cancer cells, U11 snRNA knockout and overexpressed cell lines were constructed and further used to analyze the gene expression changes by RNA sequencing. The differentially expressed genes were found to be mainly enriched in tumor-related pathways both in the U11 knockout and overexpression cell lines, such as NF-kappa B signaling pathway, bladder cancer and PI3K-Akt signaling pathway. Furthermore, alternative splicing events were proposed to participate in the potential regulatory mechanism induced by the U11 knockout or overexpression. In conclusion, U11 may be involved in the regulation of gene expression in bladder cancer cells, which may provide a potentially new biomarker for clinical diagnosis and treatment of bladder cancer. Frontiers Media S.A. 2021-07-02 /pmc/articles/PMC8283811/ /pubmed/34276798 http://dx.doi.org/10.3389/fgene.2021.695597 Text en Copyright © 2021 Wang, Wang, Wang, Tang, Feng, Pan, Lu, Tao, Xie, Wang and Tang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Wang, Zhenxing Wang, Xi Wang, Yaobang Tang, Shaomei Feng, Chao Pan, Lixin Lu, Qinchen Tao, Yuting Xie, Yuanliang Wang, Qiuyan Tang, Zhong Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer |
title | Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer |
title_full | Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer |
title_fullStr | Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer |
title_full_unstemmed | Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer |
title_short | Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer |
title_sort | transcriptomic analysis of gene networks regulated by u11 small nuclear rna in bladder cancer |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283811/ https://www.ncbi.nlm.nih.gov/pubmed/34276798 http://dx.doi.org/10.3389/fgene.2021.695597 |
work_keys_str_mv | AT wangzhenxing transcriptomicanalysisofgenenetworksregulatedbyu11smallnuclearrnainbladdercancer AT wangxi transcriptomicanalysisofgenenetworksregulatedbyu11smallnuclearrnainbladdercancer AT wangyaobang transcriptomicanalysisofgenenetworksregulatedbyu11smallnuclearrnainbladdercancer AT tangshaomei transcriptomicanalysisofgenenetworksregulatedbyu11smallnuclearrnainbladdercancer AT fengchao transcriptomicanalysisofgenenetworksregulatedbyu11smallnuclearrnainbladdercancer AT panlixin transcriptomicanalysisofgenenetworksregulatedbyu11smallnuclearrnainbladdercancer AT luqinchen transcriptomicanalysisofgenenetworksregulatedbyu11smallnuclearrnainbladdercancer AT taoyuting transcriptomicanalysisofgenenetworksregulatedbyu11smallnuclearrnainbladdercancer AT xieyuanliang transcriptomicanalysisofgenenetworksregulatedbyu11smallnuclearrnainbladdercancer AT wangqiuyan transcriptomicanalysisofgenenetworksregulatedbyu11smallnuclearrnainbladdercancer AT tangzhong transcriptomicanalysisofgenenetworksregulatedbyu11smallnuclearrnainbladdercancer |