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Evaluation of automated antigen detection test for detection of SARS-CoV-2

RT-PCR is the gold standard to detect SARS-CoV-2, however, its capacity is limited. We evaluated an automated antigen detection (AAD) test, Elecsys SARS-CoV-2 Antigen (Roche, Germany), for detecting SARS-CoV-2. We compared the limit of detection (LOD) between AAD test, rapid antigen detection (RAD)...

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Autores principales: Mak, Gannon C.K., Lau, Stephen S.Y., Wong, Kitty K.Y., Chow, Nancy L.S., Lau, C.S., Ng, Ken H.L., Lam, Edman T.K., Chan, Rickjason C.W., Tsang, Dominic N.C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284070/
https://www.ncbi.nlm.nih.gov/pubmed/34399380
http://dx.doi.org/10.1016/j.diagmicrobio.2021.115490
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author Mak, Gannon C.K.
Lau, Stephen S.Y.
Wong, Kitty K.Y.
Chow, Nancy L.S.
Lau, C.S.
Ng, Ken H.L.
Lam, Edman T.K.
Chan, Rickjason C.W.
Tsang, Dominic N.C.
author_facet Mak, Gannon C.K.
Lau, Stephen S.Y.
Wong, Kitty K.Y.
Chow, Nancy L.S.
Lau, C.S.
Ng, Ken H.L.
Lam, Edman T.K.
Chan, Rickjason C.W.
Tsang, Dominic N.C.
author_sort Mak, Gannon C.K.
collection PubMed
description RT-PCR is the gold standard to detect SARS-CoV-2, however, its capacity is limited. We evaluated an automated antigen detection (AAD) test, Elecsys SARS-CoV-2 Antigen (Roche, Germany), for detecting SARS-CoV-2. We compared the limit of detection (LOD) between AAD test, rapid antigen detection (RAD) test; SARS-CoV-2 Rapid Antigen Test (SD Biosensor, Korea), and in-house RT-PCR test. LOD results showed that the AAD test was 100 fold more sensitive than the RAD test, while the sensitivity of the AAD test was comparable to the RT-PCR test. The AAD test detected between 85.7% and 88.6% of RT-PCR-positive specimens collected from COVID-19 patients, false negative results were observed for specimens with Ct values >30. Although clinical sensitivity for the AAD test was not superior or comparable to the RT-PCR test in the present study, the AAD test may be an alternative to RT-PCR test in terms of turn-around time and throughput.
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spelling pubmed-82840702021-07-20 Evaluation of automated antigen detection test for detection of SARS-CoV-2 Mak, Gannon C.K. Lau, Stephen S.Y. Wong, Kitty K.Y. Chow, Nancy L.S. Lau, C.S. Ng, Ken H.L. Lam, Edman T.K. Chan, Rickjason C.W. Tsang, Dominic N.C. Diagn Microbiol Infect Dis Virology RT-PCR is the gold standard to detect SARS-CoV-2, however, its capacity is limited. We evaluated an automated antigen detection (AAD) test, Elecsys SARS-CoV-2 Antigen (Roche, Germany), for detecting SARS-CoV-2. We compared the limit of detection (LOD) between AAD test, rapid antigen detection (RAD) test; SARS-CoV-2 Rapid Antigen Test (SD Biosensor, Korea), and in-house RT-PCR test. LOD results showed that the AAD test was 100 fold more sensitive than the RAD test, while the sensitivity of the AAD test was comparable to the RT-PCR test. The AAD test detected between 85.7% and 88.6% of RT-PCR-positive specimens collected from COVID-19 patients, false negative results were observed for specimens with Ct values >30. Although clinical sensitivity for the AAD test was not superior or comparable to the RT-PCR test in the present study, the AAD test may be an alternative to RT-PCR test in terms of turn-around time and throughput. Elsevier Inc. 2021-12 2021-07-16 /pmc/articles/PMC8284070/ /pubmed/34399380 http://dx.doi.org/10.1016/j.diagmicrobio.2021.115490 Text en © 2021 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Virology
Mak, Gannon C.K.
Lau, Stephen S.Y.
Wong, Kitty K.Y.
Chow, Nancy L.S.
Lau, C.S.
Ng, Ken H.L.
Lam, Edman T.K.
Chan, Rickjason C.W.
Tsang, Dominic N.C.
Evaluation of automated antigen detection test for detection of SARS-CoV-2
title Evaluation of automated antigen detection test for detection of SARS-CoV-2
title_full Evaluation of automated antigen detection test for detection of SARS-CoV-2
title_fullStr Evaluation of automated antigen detection test for detection of SARS-CoV-2
title_full_unstemmed Evaluation of automated antigen detection test for detection of SARS-CoV-2
title_short Evaluation of automated antigen detection test for detection of SARS-CoV-2
title_sort evaluation of automated antigen detection test for detection of sars-cov-2
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284070/
https://www.ncbi.nlm.nih.gov/pubmed/34399380
http://dx.doi.org/10.1016/j.diagmicrobio.2021.115490
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