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Treg cells in the course of chronic hepatitis C virus infection partially normalize in longitudinal observation after successful DAA treatment regardless of hepatic fibrosis stage
AIM OF THE STUDY: Elevated circulating CD4+ CD25+ Foxp3+ regulatory T cells in patients with chronic hepatitis C (CHC) play an unspecified role in liver fibrosis development. This study aimed to determine whether Treg cells diminish after successful treatment with directacting antivirals (DAA) in pa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284172/ https://www.ncbi.nlm.nih.gov/pubmed/34295988 http://dx.doi.org/10.5114/ceh.2021.107122 |
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author | Zientarska, Agata Kaczmarek, Mariusz Mozer-Lisewska, Iwona Kowala-Piaskowska, Arleta Witkowska, Aleksandra Żeromski, Jan |
author_facet | Zientarska, Agata Kaczmarek, Mariusz Mozer-Lisewska, Iwona Kowala-Piaskowska, Arleta Witkowska, Aleksandra Żeromski, Jan |
author_sort | Zientarska, Agata |
collection | PubMed |
description | AIM OF THE STUDY: Elevated circulating CD4+ CD25+ Foxp3+ regulatory T cells in patients with chronic hepatitis C (CHC) play an unspecified role in liver fibrosis development. This study aimed to determine whether Treg cells diminish after successful treatment with directacting antivirals (DAA) in patients at different liver fibrosis stages. MATERIAL AND METHODS: We examined 44 patients with CHC (including 29 with liver cirrhosis) seven days before DAA treatment (T0), six months later (T1) and then 22 of them were examined one year (T2) after the first dose. Subsequently, these were compared with 28 volunteers without hepatitis C virus (HCV) (15 with excessive alcohol intake). We assessed the degree of liver fibrosis with FibroScan, aspartate transaminase (AST) to platelet ratio index (APRI), FibroIndex, the Forns index and Fib-4. Circulating Treg cells were measured using flow cytometry. RESULTS: All patients achieved a sustained virological response (SVR). After the treatment, all liver fibrosis indicators decreased significantly. The number of circulating Tregs was lower in healthy controls than in patients with CHC (0.0066 × 10(3) cells/µl and 0.0084 × 10(3) cells/µl, respectively, p = 0.048). After the treatment we observed an insignificant change to 0.0047 × 10(3) cells/µl for T1 (p > 0.05) and a significant fall to 0.0041 × 10(3) cells/µl for T2 (p = 0.03). There was no correlation between the degree of hepatic fibrosis and number of Tregs or post-treatment dynamics. CONCLUSIONS: Our study shows that Treg cells normalize gradually over a prolonged period of time after a successful DAA treatment. Their number and dynamics remain independent of liver fibrosis degree. The correlation of this revelation with metabolic disorders, increased susceptibility to infections or persistent risk of HCC remains unclear. |
format | Online Article Text |
id | pubmed-8284172 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-82841722021-07-21 Treg cells in the course of chronic hepatitis C virus infection partially normalize in longitudinal observation after successful DAA treatment regardless of hepatic fibrosis stage Zientarska, Agata Kaczmarek, Mariusz Mozer-Lisewska, Iwona Kowala-Piaskowska, Arleta Witkowska, Aleksandra Żeromski, Jan Clin Exp Hepatol Original Paper AIM OF THE STUDY: Elevated circulating CD4+ CD25+ Foxp3+ regulatory T cells in patients with chronic hepatitis C (CHC) play an unspecified role in liver fibrosis development. This study aimed to determine whether Treg cells diminish after successful treatment with directacting antivirals (DAA) in patients at different liver fibrosis stages. MATERIAL AND METHODS: We examined 44 patients with CHC (including 29 with liver cirrhosis) seven days before DAA treatment (T0), six months later (T1) and then 22 of them were examined one year (T2) after the first dose. Subsequently, these were compared with 28 volunteers without hepatitis C virus (HCV) (15 with excessive alcohol intake). We assessed the degree of liver fibrosis with FibroScan, aspartate transaminase (AST) to platelet ratio index (APRI), FibroIndex, the Forns index and Fib-4. Circulating Treg cells were measured using flow cytometry. RESULTS: All patients achieved a sustained virological response (SVR). After the treatment, all liver fibrosis indicators decreased significantly. The number of circulating Tregs was lower in healthy controls than in patients with CHC (0.0066 × 10(3) cells/µl and 0.0084 × 10(3) cells/µl, respectively, p = 0.048). After the treatment we observed an insignificant change to 0.0047 × 10(3) cells/µl for T1 (p > 0.05) and a significant fall to 0.0041 × 10(3) cells/µl for T2 (p = 0.03). There was no correlation between the degree of hepatic fibrosis and number of Tregs or post-treatment dynamics. CONCLUSIONS: Our study shows that Treg cells normalize gradually over a prolonged period of time after a successful DAA treatment. Their number and dynamics remain independent of liver fibrosis degree. The correlation of this revelation with metabolic disorders, increased susceptibility to infections or persistent risk of HCC remains unclear. Termedia Publishing House 2021-06-30 2021-06 /pmc/articles/PMC8284172/ /pubmed/34295988 http://dx.doi.org/10.5114/ceh.2021.107122 Text en Copyright © 2021 Clinical and Experimental Hepatology https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0). License (http://creativecommons.org/licenses/by-nc-sa/4.0/ (https://creativecommons.org/licenses/by-nc-sa/4.0/) ) |
spellingShingle | Original Paper Zientarska, Agata Kaczmarek, Mariusz Mozer-Lisewska, Iwona Kowala-Piaskowska, Arleta Witkowska, Aleksandra Żeromski, Jan Treg cells in the course of chronic hepatitis C virus infection partially normalize in longitudinal observation after successful DAA treatment regardless of hepatic fibrosis stage |
title | Treg cells in the course of chronic hepatitis C virus infection partially normalize in longitudinal observation after successful DAA treatment regardless of hepatic fibrosis stage |
title_full | Treg cells in the course of chronic hepatitis C virus infection partially normalize in longitudinal observation after successful DAA treatment regardless of hepatic fibrosis stage |
title_fullStr | Treg cells in the course of chronic hepatitis C virus infection partially normalize in longitudinal observation after successful DAA treatment regardless of hepatic fibrosis stage |
title_full_unstemmed | Treg cells in the course of chronic hepatitis C virus infection partially normalize in longitudinal observation after successful DAA treatment regardless of hepatic fibrosis stage |
title_short | Treg cells in the course of chronic hepatitis C virus infection partially normalize in longitudinal observation after successful DAA treatment regardless of hepatic fibrosis stage |
title_sort | treg cells in the course of chronic hepatitis c virus infection partially normalize in longitudinal observation after successful daa treatment regardless of hepatic fibrosis stage |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284172/ https://www.ncbi.nlm.nih.gov/pubmed/34295988 http://dx.doi.org/10.5114/ceh.2021.107122 |
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