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Dexmedetomidine may decrease the bupivacaine toxicity to heart

OBJECTIVE: The purpose of our study was to explore the effect of dexmedetomidine on cardiac tolerance to bupivacaine. METHOD: Human coronary endothelial cells were used to establish in vitro model. They were randomly divided into control (Con) group, dexmedetomidine (Dex) group, bupivacaine (Bupi) g...

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Autores principales: Jin, Zhousheng, Xia, Fangfang, Lin, Tingting, Cai, Yaoyao, Chen, Hongfei, Wang, Yuelan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: De Gruyter 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284331/
https://www.ncbi.nlm.nih.gov/pubmed/34307889
http://dx.doi.org/10.1515/med-2021-0311
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author Jin, Zhousheng
Xia, Fangfang
Lin, Tingting
Cai, Yaoyao
Chen, Hongfei
Wang, Yuelan
author_facet Jin, Zhousheng
Xia, Fangfang
Lin, Tingting
Cai, Yaoyao
Chen, Hongfei
Wang, Yuelan
author_sort Jin, Zhousheng
collection PubMed
description OBJECTIVE: The purpose of our study was to explore the effect of dexmedetomidine on cardiac tolerance to bupivacaine. METHOD: Human coronary endothelial cells were used to establish in vitro model. They were randomly divided into control (Con) group, dexmedetomidine (Dex) group, bupivacaine (Bupi) group, dexmedetomidine + bupivacaine group (DB group), and dexmedetomidine + bupivacaine + PI3K inhibitor (DB-inhibitor) group. Cell activity was measured by Cell counting kit-8 (CCK-8). Transwell was used to detect cell permeability. Western blotting was used to detect the protein expression of related factors. RESULTS: There were no notable differences in cell activity among the five groups (P > 0.05). Dexmedetomidine significantly reduced the permeability of endothelial cells to bupivacaine and increased the protein expression of Zonulaoeeludens-1 (ZO-1) (P < 0.01). However, the aforementioned effects of dexmedetomidine were disappeared after the addition of PI3K inhibitors. Furthermore, Dex and DB markedly increased the protein expression of PI3K, p-Akt, and p-PTEN in comparison with Con group (P < 0.001), but there was no significant difference in p-PTEN among DB-inhibitor, Con, and Bupi groups (P > 0.05). CONCLUSION: Dex reduced Bupi-induced vasopermeability through protein expression of ZO-1 and PI3K/Akt pathway, which may lead to the decrease of Bupi-induced cardiotoxicity.
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spelling pubmed-82843312021-07-23 Dexmedetomidine may decrease the bupivacaine toxicity to heart Jin, Zhousheng Xia, Fangfang Lin, Tingting Cai, Yaoyao Chen, Hongfei Wang, Yuelan Open Med (Wars) Research Article OBJECTIVE: The purpose of our study was to explore the effect of dexmedetomidine on cardiac tolerance to bupivacaine. METHOD: Human coronary endothelial cells were used to establish in vitro model. They were randomly divided into control (Con) group, dexmedetomidine (Dex) group, bupivacaine (Bupi) group, dexmedetomidine + bupivacaine group (DB group), and dexmedetomidine + bupivacaine + PI3K inhibitor (DB-inhibitor) group. Cell activity was measured by Cell counting kit-8 (CCK-8). Transwell was used to detect cell permeability. Western blotting was used to detect the protein expression of related factors. RESULTS: There were no notable differences in cell activity among the five groups (P > 0.05). Dexmedetomidine significantly reduced the permeability of endothelial cells to bupivacaine and increased the protein expression of Zonulaoeeludens-1 (ZO-1) (P < 0.01). However, the aforementioned effects of dexmedetomidine were disappeared after the addition of PI3K inhibitors. Furthermore, Dex and DB markedly increased the protein expression of PI3K, p-Akt, and p-PTEN in comparison with Con group (P < 0.001), but there was no significant difference in p-PTEN among DB-inhibitor, Con, and Bupi groups (P > 0.05). CONCLUSION: Dex reduced Bupi-induced vasopermeability through protein expression of ZO-1 and PI3K/Akt pathway, which may lead to the decrease of Bupi-induced cardiotoxicity. De Gruyter 2021-07-15 /pmc/articles/PMC8284331/ /pubmed/34307889 http://dx.doi.org/10.1515/med-2021-0311 Text en © 2021 Zhousheng Jin et al., published by De Gruyter https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License.
spellingShingle Research Article
Jin, Zhousheng
Xia, Fangfang
Lin, Tingting
Cai, Yaoyao
Chen, Hongfei
Wang, Yuelan
Dexmedetomidine may decrease the bupivacaine toxicity to heart
title Dexmedetomidine may decrease the bupivacaine toxicity to heart
title_full Dexmedetomidine may decrease the bupivacaine toxicity to heart
title_fullStr Dexmedetomidine may decrease the bupivacaine toxicity to heart
title_full_unstemmed Dexmedetomidine may decrease the bupivacaine toxicity to heart
title_short Dexmedetomidine may decrease the bupivacaine toxicity to heart
title_sort dexmedetomidine may decrease the bupivacaine toxicity to heart
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284331/
https://www.ncbi.nlm.nih.gov/pubmed/34307889
http://dx.doi.org/10.1515/med-2021-0311
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