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BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II

BL-11C, a new protein crystallography beamline, is an in-vacuum undulator-based microfocus beamline used for macromolecular crystallography at the Pohang Accelerator Laboratory and it was made available to users in June 2017. The beamline is energy tunable in the range 5.0–20 keV to support conventi...

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Autores principales: Gu, Do-Heon, Eo, Cheolsoo, Hwangbo, Seung-A, Ha, Sung-Chul, Kim, Jin Hong, Kim, Hyoyun, Lee, Chae-Soon, Seo, In Deuk, Yun, Young Duck, Lee, Woulwoo, Choi, Hyeongju, Kim, Jangwoo, Lim, Jun, Rah, Seungyu, Kim, Jeong-Sun, Lee, Jie-Oh, Kim, Yeon-Gil, Park, Suk-Youl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284399/
https://www.ncbi.nlm.nih.gov/pubmed/34212886
http://dx.doi.org/10.1107/S1600577521004355
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author Gu, Do-Heon
Eo, Cheolsoo
Hwangbo, Seung-A
Ha, Sung-Chul
Kim, Jin Hong
Kim, Hyoyun
Lee, Chae-Soon
Seo, In Deuk
Yun, Young Duck
Lee, Woulwoo
Choi, Hyeongju
Kim, Jangwoo
Lim, Jun
Rah, Seungyu
Kim, Jeong-Sun
Lee, Jie-Oh
Kim, Yeon-Gil
Park, Suk-Youl
author_facet Gu, Do-Heon
Eo, Cheolsoo
Hwangbo, Seung-A
Ha, Sung-Chul
Kim, Jin Hong
Kim, Hyoyun
Lee, Chae-Soon
Seo, In Deuk
Yun, Young Duck
Lee, Woulwoo
Choi, Hyeongju
Kim, Jangwoo
Lim, Jun
Rah, Seungyu
Kim, Jeong-Sun
Lee, Jie-Oh
Kim, Yeon-Gil
Park, Suk-Youl
author_sort Gu, Do-Heon
collection PubMed
description BL-11C, a new protein crystallography beamline, is an in-vacuum undulator-based microfocus beamline used for macromolecular crystallography at the Pohang Accelerator Laboratory and it was made available to users in June 2017. The beamline is energy tunable in the range 5.0–20 keV to support conventional single- and multi-wavelength anomalous-dispersion experiments against a wide range of heavy metals. At the standard working energy of 12.659 keV, the monochromated beam is focused to 4.1 µm (V) × 8.5 µm (H) full width at half-maximum at the sample position and the measured photon flux is 1.3 × 10(12) photons s(−1). The experimental station is equipped with a Pilatus3 6M detector, a micro-diffractometer (MD2S) incorporating a multi-axis goniometer, and a robotic sample exchanger (CATS) with a dewar capacity of 90 samples. This beamline is suitable for structural determination of weakly diffracting crystalline substances, such as biomaterials, including protein, nucleic acids and their complexes. In addition, serial crystallography experiments for determining crystal structures at room temperature are possible. Herein, the current beamline characteristics, technical information for users and some recent scientific highlights are described.
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spelling pubmed-82843992021-08-02 BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II Gu, Do-Heon Eo, Cheolsoo Hwangbo, Seung-A Ha, Sung-Chul Kim, Jin Hong Kim, Hyoyun Lee, Chae-Soon Seo, In Deuk Yun, Young Duck Lee, Woulwoo Choi, Hyeongju Kim, Jangwoo Lim, Jun Rah, Seungyu Kim, Jeong-Sun Lee, Jie-Oh Kim, Yeon-Gil Park, Suk-Youl J Synchrotron Radiat Beamlines BL-11C, a new protein crystallography beamline, is an in-vacuum undulator-based microfocus beamline used for macromolecular crystallography at the Pohang Accelerator Laboratory and it was made available to users in June 2017. The beamline is energy tunable in the range 5.0–20 keV to support conventional single- and multi-wavelength anomalous-dispersion experiments against a wide range of heavy metals. At the standard working energy of 12.659 keV, the monochromated beam is focused to 4.1 µm (V) × 8.5 µm (H) full width at half-maximum at the sample position and the measured photon flux is 1.3 × 10(12) photons s(−1). The experimental station is equipped with a Pilatus3 6M detector, a micro-diffractometer (MD2S) incorporating a multi-axis goniometer, and a robotic sample exchanger (CATS) with a dewar capacity of 90 samples. This beamline is suitable for structural determination of weakly diffracting crystalline substances, such as biomaterials, including protein, nucleic acids and their complexes. In addition, serial crystallography experiments for determining crystal structures at room temperature are possible. Herein, the current beamline characteristics, technical information for users and some recent scientific highlights are described. International Union of Crystallography 2021-06-01 /pmc/articles/PMC8284399/ /pubmed/34212886 http://dx.doi.org/10.1107/S1600577521004355 Text en © Do-Heon Gu et al. 2021 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Beamlines
Gu, Do-Heon
Eo, Cheolsoo
Hwangbo, Seung-A
Ha, Sung-Chul
Kim, Jin Hong
Kim, Hyoyun
Lee, Chae-Soon
Seo, In Deuk
Yun, Young Duck
Lee, Woulwoo
Choi, Hyeongju
Kim, Jangwoo
Lim, Jun
Rah, Seungyu
Kim, Jeong-Sun
Lee, Jie-Oh
Kim, Yeon-Gil
Park, Suk-Youl
BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II
title BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II
title_full BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II
title_fullStr BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II
title_full_unstemmed BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II
title_short BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II
title_sort bl-11c micro-mx: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at pohang light source ii
topic Beamlines
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284399/
https://www.ncbi.nlm.nih.gov/pubmed/34212886
http://dx.doi.org/10.1107/S1600577521004355
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