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BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II
BL-11C, a new protein crystallography beamline, is an in-vacuum undulator-based microfocus beamline used for macromolecular crystallography at the Pohang Accelerator Laboratory and it was made available to users in June 2017. The beamline is energy tunable in the range 5.0–20 keV to support conventi...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284399/ https://www.ncbi.nlm.nih.gov/pubmed/34212886 http://dx.doi.org/10.1107/S1600577521004355 |
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author | Gu, Do-Heon Eo, Cheolsoo Hwangbo, Seung-A Ha, Sung-Chul Kim, Jin Hong Kim, Hyoyun Lee, Chae-Soon Seo, In Deuk Yun, Young Duck Lee, Woulwoo Choi, Hyeongju Kim, Jangwoo Lim, Jun Rah, Seungyu Kim, Jeong-Sun Lee, Jie-Oh Kim, Yeon-Gil Park, Suk-Youl |
author_facet | Gu, Do-Heon Eo, Cheolsoo Hwangbo, Seung-A Ha, Sung-Chul Kim, Jin Hong Kim, Hyoyun Lee, Chae-Soon Seo, In Deuk Yun, Young Duck Lee, Woulwoo Choi, Hyeongju Kim, Jangwoo Lim, Jun Rah, Seungyu Kim, Jeong-Sun Lee, Jie-Oh Kim, Yeon-Gil Park, Suk-Youl |
author_sort | Gu, Do-Heon |
collection | PubMed |
description | BL-11C, a new protein crystallography beamline, is an in-vacuum undulator-based microfocus beamline used for macromolecular crystallography at the Pohang Accelerator Laboratory and it was made available to users in June 2017. The beamline is energy tunable in the range 5.0–20 keV to support conventional single- and multi-wavelength anomalous-dispersion experiments against a wide range of heavy metals. At the standard working energy of 12.659 keV, the monochromated beam is focused to 4.1 µm (V) × 8.5 µm (H) full width at half-maximum at the sample position and the measured photon flux is 1.3 × 10(12) photons s(−1). The experimental station is equipped with a Pilatus3 6M detector, a micro-diffractometer (MD2S) incorporating a multi-axis goniometer, and a robotic sample exchanger (CATS) with a dewar capacity of 90 samples. This beamline is suitable for structural determination of weakly diffracting crystalline substances, such as biomaterials, including protein, nucleic acids and their complexes. In addition, serial crystallography experiments for determining crystal structures at room temperature are possible. Herein, the current beamline characteristics, technical information for users and some recent scientific highlights are described. |
format | Online Article Text |
id | pubmed-8284399 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-82843992021-08-02 BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II Gu, Do-Heon Eo, Cheolsoo Hwangbo, Seung-A Ha, Sung-Chul Kim, Jin Hong Kim, Hyoyun Lee, Chae-Soon Seo, In Deuk Yun, Young Duck Lee, Woulwoo Choi, Hyeongju Kim, Jangwoo Lim, Jun Rah, Seungyu Kim, Jeong-Sun Lee, Jie-Oh Kim, Yeon-Gil Park, Suk-Youl J Synchrotron Radiat Beamlines BL-11C, a new protein crystallography beamline, is an in-vacuum undulator-based microfocus beamline used for macromolecular crystallography at the Pohang Accelerator Laboratory and it was made available to users in June 2017. The beamline is energy tunable in the range 5.0–20 keV to support conventional single- and multi-wavelength anomalous-dispersion experiments against a wide range of heavy metals. At the standard working energy of 12.659 keV, the monochromated beam is focused to 4.1 µm (V) × 8.5 µm (H) full width at half-maximum at the sample position and the measured photon flux is 1.3 × 10(12) photons s(−1). The experimental station is equipped with a Pilatus3 6M detector, a micro-diffractometer (MD2S) incorporating a multi-axis goniometer, and a robotic sample exchanger (CATS) with a dewar capacity of 90 samples. This beamline is suitable for structural determination of weakly diffracting crystalline substances, such as biomaterials, including protein, nucleic acids and their complexes. In addition, serial crystallography experiments for determining crystal structures at room temperature are possible. Herein, the current beamline characteristics, technical information for users and some recent scientific highlights are described. International Union of Crystallography 2021-06-01 /pmc/articles/PMC8284399/ /pubmed/34212886 http://dx.doi.org/10.1107/S1600577521004355 Text en © Do-Heon Gu et al. 2021 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Beamlines Gu, Do-Heon Eo, Cheolsoo Hwangbo, Seung-A Ha, Sung-Chul Kim, Jin Hong Kim, Hyoyun Lee, Chae-Soon Seo, In Deuk Yun, Young Duck Lee, Woulwoo Choi, Hyeongju Kim, Jangwoo Lim, Jun Rah, Seungyu Kim, Jeong-Sun Lee, Jie-Oh Kim, Yeon-Gil Park, Suk-Youl BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II |
title | BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II |
title_full | BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II |
title_fullStr | BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II |
title_full_unstemmed | BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II |
title_short | BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II |
title_sort | bl-11c micro-mx: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at pohang light source ii |
topic | Beamlines |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284399/ https://www.ncbi.nlm.nih.gov/pubmed/34212886 http://dx.doi.org/10.1107/S1600577521004355 |
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