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Analysis of electrostatic coupling throughout the laboratory evolution of a designed retroaldolase

The roles of local interactions in the laboratory evolution of a highly active, computationally designed retroaldolase (RA) are examined. Partial Order Optimum Likelihood (POOL) is used to identify catalytically important amino acid interactions in several RA95 enzyme variants. The series RA95.5, RA...

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Detalles Bibliográficos
Autores principales:	Coulther, Timothy A., Pott, Moritz, Zeymer, Cathleen, Hilvert, Donald, Ondrechen, Mary Jo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley & Sons, Inc. 2021
Materias:	Full‐Length Papers
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284568/ https://www.ncbi.nlm.nih.gov/pubmed/33938058 http://dx.doi.org/10.1002/pro.4099

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