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CRISPR/Cas13d-mediated efficient KDM5B mRNA knockdown in porcine somatic cells and parthenogenetic embryos

An efficient mRNA knockdown strategy is needed to explore gene function in cells and embryos, especially to understand the process of maternal mRNA decay during early embryo development. Cas13, a novel RNA-targeting CRISPR effector protein, could bind and cleave complementary single-strand RNA, whic...

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Detalles Bibliográficos
Autores principales:	Bi, Dengfeng, Yao, Jing, Wang, Yu, Qin, Guosong, Zhang, Yunting, Wang, Yanfang, Zhao, Jianguo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Bioscientifica Ltd 2021
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284906/ https://www.ncbi.nlm.nih.gov/pubmed/34096883 http://dx.doi.org/10.1530/REP-21-0053

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