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Protein transduction domain-mediated influenza NP subunit vaccine generates a potent immune response and protection against influenza virus in mice
The nucleoprotein (NP) is a highly conserved internal protein of the influenza virus, a major target for universal influenza vaccine. Our previous studies have proven NP-based subunit vaccine can provide partial protection in mice. It is reported that the protein transduction domain (PTD) TAT protei...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Taylor & Francis
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284974/ https://www.ncbi.nlm.nih.gov/pubmed/32811334 http://dx.doi.org/10.1080/22221751.2020.1812436 |
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author | Yin, Yuan Li, BeiBei Zhou, Linting Luo, Jian Liu, Xueying Wang, Shilei Lu, Qun Tan, Wensong Chen, Ze |
author_facet | Yin, Yuan Li, BeiBei Zhou, Linting Luo, Jian Liu, Xueying Wang, Shilei Lu, Qun Tan, Wensong Chen, Ze |
author_sort | Yin, Yuan |
collection | PubMed |
description | The nucleoprotein (NP) is a highly conserved internal protein of the influenza virus, a major target for universal influenza vaccine. Our previous studies have proven NP-based subunit vaccine can provide partial protection in mice. It is reported that the protein transduction domain (PTD) TAT protein from human immunodeficiency virus-1 (HIV-1) is able to penetrate cells when added exogenous protein and could effectively enhance the immune response induced by the exogenous protein. In present study, the recombinant protein TAT-NP, a fusion of TAT and NP was effectively expressed in Escherichia coli and purified as a candidate component for an influenza vaccine. We evaluated the immunogenicity and protective efficacy of recombinant influenza TAT-NP vaccine by intranasal immunization. In vitro experiments showed that TAT-NP could efficiently penetrate into cells. Animal results showed that mice vaccinated with TAT-NP could not only induce higher levels of IgG and mucosal IgA, but also elicit a robust cellular immune response. Moreover, the TAT-NP fusion protein could significantly increase the protection of mice against lethal doses of homologous influenza virus PR8 and could also provide mice protection against a lethal dose challenge against heterosubtypic H9N2 and H3N2 influenza virus. In conclusion, the recombinant TAT-NP might be a universal vaccine candidate against influenza virus. |
format | Online Article Text |
id | pubmed-8284974 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-82849742021-08-02 Protein transduction domain-mediated influenza NP subunit vaccine generates a potent immune response and protection against influenza virus in mice Yin, Yuan Li, BeiBei Zhou, Linting Luo, Jian Liu, Xueying Wang, Shilei Lu, Qun Tan, Wensong Chen, Ze Emerg Microbes Infect Articles The nucleoprotein (NP) is a highly conserved internal protein of the influenza virus, a major target for universal influenza vaccine. Our previous studies have proven NP-based subunit vaccine can provide partial protection in mice. It is reported that the protein transduction domain (PTD) TAT protein from human immunodeficiency virus-1 (HIV-1) is able to penetrate cells when added exogenous protein and could effectively enhance the immune response induced by the exogenous protein. In present study, the recombinant protein TAT-NP, a fusion of TAT and NP was effectively expressed in Escherichia coli and purified as a candidate component for an influenza vaccine. We evaluated the immunogenicity and protective efficacy of recombinant influenza TAT-NP vaccine by intranasal immunization. In vitro experiments showed that TAT-NP could efficiently penetrate into cells. Animal results showed that mice vaccinated with TAT-NP could not only induce higher levels of IgG and mucosal IgA, but also elicit a robust cellular immune response. Moreover, the TAT-NP fusion protein could significantly increase the protection of mice against lethal doses of homologous influenza virus PR8 and could also provide mice protection against a lethal dose challenge against heterosubtypic H9N2 and H3N2 influenza virus. In conclusion, the recombinant TAT-NP might be a universal vaccine candidate against influenza virus. Taylor & Francis 2020-09-02 /pmc/articles/PMC8284974/ /pubmed/32811334 http://dx.doi.org/10.1080/22221751.2020.1812436 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Yin, Yuan Li, BeiBei Zhou, Linting Luo, Jian Liu, Xueying Wang, Shilei Lu, Qun Tan, Wensong Chen, Ze Protein transduction domain-mediated influenza NP subunit vaccine generates a potent immune response and protection against influenza virus in mice |
title | Protein transduction domain-mediated influenza NP subunit vaccine generates a potent immune response and protection against influenza virus in mice |
title_full | Protein transduction domain-mediated influenza NP subunit vaccine generates a potent immune response and protection against influenza virus in mice |
title_fullStr | Protein transduction domain-mediated influenza NP subunit vaccine generates a potent immune response and protection against influenza virus in mice |
title_full_unstemmed | Protein transduction domain-mediated influenza NP subunit vaccine generates a potent immune response and protection against influenza virus in mice |
title_short | Protein transduction domain-mediated influenza NP subunit vaccine generates a potent immune response and protection against influenza virus in mice |
title_sort | protein transduction domain-mediated influenza np subunit vaccine generates a potent immune response and protection against influenza virus in mice |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284974/ https://www.ncbi.nlm.nih.gov/pubmed/32811334 http://dx.doi.org/10.1080/22221751.2020.1812436 |
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