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Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation

Cleft lip and palate (CL/P) is the most prevalent craniofacial birth defect in humans. None of the surgical procedures currently used for CL/P repair lead to definitive correction of hard palate bone interruption. Advances in tissue engineering and regenerative medicine aim to develop new strategies...

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Autores principales: Rizzo, M. I., Tomao, L., Tedesco, S., Cajozzo, M., Esposito, M., De Stefanis, C., Ferranti, A. M., Mezzogori, D., Palmieri, A., Pozzato, G., Algeri, M., Locatelli, F., Leone, L., Zama, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285425/
https://www.ncbi.nlm.nih.gov/pubmed/34272436
http://dx.doi.org/10.1038/s41598-021-93951-w
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author Rizzo, M. I.
Tomao, L.
Tedesco, S.
Cajozzo, M.
Esposito, M.
De Stefanis, C.
Ferranti, A. M.
Mezzogori, D.
Palmieri, A.
Pozzato, G.
Algeri, M.
Locatelli, F.
Leone, L.
Zama, M.
author_facet Rizzo, M. I.
Tomao, L.
Tedesco, S.
Cajozzo, M.
Esposito, M.
De Stefanis, C.
Ferranti, A. M.
Mezzogori, D.
Palmieri, A.
Pozzato, G.
Algeri, M.
Locatelli, F.
Leone, L.
Zama, M.
author_sort Rizzo, M. I.
collection PubMed
description Cleft lip and palate (CL/P) is the most prevalent craniofacial birth defect in humans. None of the surgical procedures currently used for CL/P repair lead to definitive correction of hard palate bone interruption. Advances in tissue engineering and regenerative medicine aim to develop new strategies to restore palatal bone interruption by using tissue or organ-decellularized bioscaffolds seeded with host cells. Aim of this study was to set up a new natural scaffold deriving from a decellularized porcine mucoperiosteum, engineered by an innovative micro-perforation procedure based on Quantum Molecular Resonance (QMR) and then subjected to in vitro recellularization with human bone marrow-derived mesenchymal stem cells (hBM-MSCs). Our results demonstrated the efficiency of decellularization treatment gaining a natural, non-immunogenic scaffold with preserved collagen microenvironment that displays a favorable support to hMSC engraftment, spreading and differentiation. Ultrastructural analysis showed that the micro-perforation procedure preserved the collagen mesh, increasing the osteoinductive potential for mesenchymal precursor cells. In conclusion, we developed a novel tissue engineering protocol to obtain a non-immunogenic mucoperiosteal scaffold suitable for allogenic transplantation and CL/P repair. The innovative micro-perforation procedure improving hMSC osteogenic differentiation potentially impacts for enhanced palatal bone regeneration leading to future clinical applications in humans.
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spelling pubmed-82854252021-07-19 Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation Rizzo, M. I. Tomao, L. Tedesco, S. Cajozzo, M. Esposito, M. De Stefanis, C. Ferranti, A. M. Mezzogori, D. Palmieri, A. Pozzato, G. Algeri, M. Locatelli, F. Leone, L. Zama, M. Sci Rep Article Cleft lip and palate (CL/P) is the most prevalent craniofacial birth defect in humans. None of the surgical procedures currently used for CL/P repair lead to definitive correction of hard palate bone interruption. Advances in tissue engineering and regenerative medicine aim to develop new strategies to restore palatal bone interruption by using tissue or organ-decellularized bioscaffolds seeded with host cells. Aim of this study was to set up a new natural scaffold deriving from a decellularized porcine mucoperiosteum, engineered by an innovative micro-perforation procedure based on Quantum Molecular Resonance (QMR) and then subjected to in vitro recellularization with human bone marrow-derived mesenchymal stem cells (hBM-MSCs). Our results demonstrated the efficiency of decellularization treatment gaining a natural, non-immunogenic scaffold with preserved collagen microenvironment that displays a favorable support to hMSC engraftment, spreading and differentiation. Ultrastructural analysis showed that the micro-perforation procedure preserved the collagen mesh, increasing the osteoinductive potential for mesenchymal precursor cells. In conclusion, we developed a novel tissue engineering protocol to obtain a non-immunogenic mucoperiosteal scaffold suitable for allogenic transplantation and CL/P repair. The innovative micro-perforation procedure improving hMSC osteogenic differentiation potentially impacts for enhanced palatal bone regeneration leading to future clinical applications in humans. Nature Publishing Group UK 2021-07-16 /pmc/articles/PMC8285425/ /pubmed/34272436 http://dx.doi.org/10.1038/s41598-021-93951-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Rizzo, M. I.
Tomao, L.
Tedesco, S.
Cajozzo, M.
Esposito, M.
De Stefanis, C.
Ferranti, A. M.
Mezzogori, D.
Palmieri, A.
Pozzato, G.
Algeri, M.
Locatelli, F.
Leone, L.
Zama, M.
Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation
title Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation
title_full Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation
title_fullStr Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation
title_full_unstemmed Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation
title_short Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation
title_sort engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285425/
https://www.ncbi.nlm.nih.gov/pubmed/34272436
http://dx.doi.org/10.1038/s41598-021-93951-w
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