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Transcriptome analysis of resistant and susceptible mulberry responses to Meloidogyne enterolobii infection

BACKGROUND: Mulberry (Morus alba L.) is an important sericulture crop; however, root-knot nematode infection seriously limits its production. Understanding the mechanism of interaction between mulberry and nematode is important for control of infection. RESULTS: Using sequencing and de novo transcri...

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Autores principales: Shao, Hudie, Fu, Yu, Zhang, Pan, You, Chunping, Li, Chuanren, Peng, Huan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285880/
https://www.ncbi.nlm.nih.gov/pubmed/34271854
http://dx.doi.org/10.1186/s12870-021-03128-w
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author Shao, Hudie
Fu, Yu
Zhang, Pan
You, Chunping
Li, Chuanren
Peng, Huan
author_facet Shao, Hudie
Fu, Yu
Zhang, Pan
You, Chunping
Li, Chuanren
Peng, Huan
author_sort Shao, Hudie
collection PubMed
description BACKGROUND: Mulberry (Morus alba L.) is an important sericulture crop; however, root-knot nematode infection seriously limits its production. Understanding the mechanism of interaction between mulberry and nematode is important for control of infection. RESULTS: Using sequencing and de novo transcriptome assembly, we identified 55,894 unigenes from root samples of resistant and susceptible mulberry cultivars at different stages after infection with the nematode Meloidogyne enterolobii; 33,987 of these were annotated in the Nr, SWISS-PROT, KEGG, and KOG databases. Gene ontology and pathway enrichment analyses of differentially expressed genes (DEGs) revealed key genes involved in hormone metabolic processes, plant hormone signal transduction, flavonoid biosynthesis, phenylpropanoid biosynthesis, and peroxisomal and photosynthetic pathways. Analysis of key trends in co-expression networks indicated that expression of unigenes 0,015,083, 0,073,272, 0,004,006, and 0,000,628 was positively correlated with resistance to M. enterolobii. Unigene 0015083 encodes tabersonine 16-O-methyltransferase (16OMT), which is involved in alkaloid biosynthesis. Unigene 0073272 encodes a transcription factor contributing to nitric oxide accumulation during plant immune responses. Unigenes 0,004,006 and 0,000,628 encode ERF and MYB transcription factors, respectively, involved in plant hormone signaling. We verified the accuracy of transcriptome sequencing results by RT-qPCR of 21 DEGs. CONCLUSIONS: The results of this study increase our understanding of the resistance mechanisms and candidate genes involved in mulberry–M. enterolobii interaction. Thus, our data will contribute to the development of effective control measures against this pathogen. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03128-w.
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spelling pubmed-82858802021-07-19 Transcriptome analysis of resistant and susceptible mulberry responses to Meloidogyne enterolobii infection Shao, Hudie Fu, Yu Zhang, Pan You, Chunping Li, Chuanren Peng, Huan BMC Plant Biol Research Article BACKGROUND: Mulberry (Morus alba L.) is an important sericulture crop; however, root-knot nematode infection seriously limits its production. Understanding the mechanism of interaction between mulberry and nematode is important for control of infection. RESULTS: Using sequencing and de novo transcriptome assembly, we identified 55,894 unigenes from root samples of resistant and susceptible mulberry cultivars at different stages after infection with the nematode Meloidogyne enterolobii; 33,987 of these were annotated in the Nr, SWISS-PROT, KEGG, and KOG databases. Gene ontology and pathway enrichment analyses of differentially expressed genes (DEGs) revealed key genes involved in hormone metabolic processes, plant hormone signal transduction, flavonoid biosynthesis, phenylpropanoid biosynthesis, and peroxisomal and photosynthetic pathways. Analysis of key trends in co-expression networks indicated that expression of unigenes 0,015,083, 0,073,272, 0,004,006, and 0,000,628 was positively correlated with resistance to M. enterolobii. Unigene 0015083 encodes tabersonine 16-O-methyltransferase (16OMT), which is involved in alkaloid biosynthesis. Unigene 0073272 encodes a transcription factor contributing to nitric oxide accumulation during plant immune responses. Unigenes 0,004,006 and 0,000,628 encode ERF and MYB transcription factors, respectively, involved in plant hormone signaling. We verified the accuracy of transcriptome sequencing results by RT-qPCR of 21 DEGs. CONCLUSIONS: The results of this study increase our understanding of the resistance mechanisms and candidate genes involved in mulberry–M. enterolobii interaction. Thus, our data will contribute to the development of effective control measures against this pathogen. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03128-w. BioMed Central 2021-07-16 /pmc/articles/PMC8285880/ /pubmed/34271854 http://dx.doi.org/10.1186/s12870-021-03128-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Shao, Hudie
Fu, Yu
Zhang, Pan
You, Chunping
Li, Chuanren
Peng, Huan
Transcriptome analysis of resistant and susceptible mulberry responses to Meloidogyne enterolobii infection
title Transcriptome analysis of resistant and susceptible mulberry responses to Meloidogyne enterolobii infection
title_full Transcriptome analysis of resistant and susceptible mulberry responses to Meloidogyne enterolobii infection
title_fullStr Transcriptome analysis of resistant and susceptible mulberry responses to Meloidogyne enterolobii infection
title_full_unstemmed Transcriptome analysis of resistant and susceptible mulberry responses to Meloidogyne enterolobii infection
title_short Transcriptome analysis of resistant and susceptible mulberry responses to Meloidogyne enterolobii infection
title_sort transcriptome analysis of resistant and susceptible mulberry responses to meloidogyne enterolobii infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285880/
https://www.ncbi.nlm.nih.gov/pubmed/34271854
http://dx.doi.org/10.1186/s12870-021-03128-w
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