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Exploration of Potential Molecular Targets of Dexmedetomidine in the Intestinal Repair of Burnt Rats

BACKGROUND: More and more burn survivors were suffering from varying degrees of damage to the intestinal barrier. Dexmedetomidine (Dex) was frequently used as sedative in more cases, but it was found to have repair effect on intestinal barrier dysfunction recently. This study aimed to explore the po...

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Autores principales: Qin, Chao, Jiang, Yi, Yu, Mingdong, Bian, Yingxue, Yu, Yonghao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286122/
https://www.ncbi.nlm.nih.gov/pubmed/34285543
http://dx.doi.org/10.2147/JIR.S315952
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author Qin, Chao
Jiang, Yi
Yu, Mingdong
Bian, Yingxue
Yu, Yonghao
author_facet Qin, Chao
Jiang, Yi
Yu, Mingdong
Bian, Yingxue
Yu, Yonghao
author_sort Qin, Chao
collection PubMed
description BACKGROUND: More and more burn survivors were suffering from varying degrees of damage to the intestinal barrier. Dexmedetomidine (Dex) was frequently used as sedative in more cases, but it was found to have repair effect on intestinal barrier dysfunction recently. This study aimed to explore the potential specific targets of Dex in intestinal barrier repair in burn rats model. METHODS: Male adult SD rats were used to establish 40% TBSA III degree scald model in our study. The samples were divided into four groups: burn rats (Burn), burn rats with Dex medication (Burn-Dex), sham rats (Sham) and sham rats with Dex medication (Sham-Dex). And plasma FITC-dextran and diamine oxidase (DAO) were detected to determine the intestinal permeability. Differentially expressed proteins were further adopted to protein–protein interaction network analysis, Gene Ontology analysis (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. RESULTS: It showed that 40% TBSA III degree scald model was successfully constructed. And plasma FITC-dextran and DAO decreased significantly after Dex administration. Additionally, differentially expressed genes Psmb10, Psmb7 among the experimental groups were screened, which were significantly enriched in proteasome and other several pathways. CONCLUSION: The results above suggested that Q4KM35 and Q9JHW0, which are encoded by Psmb10 and Psmb7, respectively, are two possible protein targets of Dex in intestinal barrier repair.
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spelling pubmed-82861222021-07-19 Exploration of Potential Molecular Targets of Dexmedetomidine in the Intestinal Repair of Burnt Rats Qin, Chao Jiang, Yi Yu, Mingdong Bian, Yingxue Yu, Yonghao J Inflamm Res Original Research BACKGROUND: More and more burn survivors were suffering from varying degrees of damage to the intestinal barrier. Dexmedetomidine (Dex) was frequently used as sedative in more cases, but it was found to have repair effect on intestinal barrier dysfunction recently. This study aimed to explore the potential specific targets of Dex in intestinal barrier repair in burn rats model. METHODS: Male adult SD rats were used to establish 40% TBSA III degree scald model in our study. The samples were divided into four groups: burn rats (Burn), burn rats with Dex medication (Burn-Dex), sham rats (Sham) and sham rats with Dex medication (Sham-Dex). And plasma FITC-dextran and diamine oxidase (DAO) were detected to determine the intestinal permeability. Differentially expressed proteins were further adopted to protein–protein interaction network analysis, Gene Ontology analysis (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. RESULTS: It showed that 40% TBSA III degree scald model was successfully constructed. And plasma FITC-dextran and DAO decreased significantly after Dex administration. Additionally, differentially expressed genes Psmb10, Psmb7 among the experimental groups were screened, which were significantly enriched in proteasome and other several pathways. CONCLUSION: The results above suggested that Q4KM35 and Q9JHW0, which are encoded by Psmb10 and Psmb7, respectively, are two possible protein targets of Dex in intestinal barrier repair. Dove 2021-07-13 /pmc/articles/PMC8286122/ /pubmed/34285543 http://dx.doi.org/10.2147/JIR.S315952 Text en © 2021 Qin et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Qin, Chao
Jiang, Yi
Yu, Mingdong
Bian, Yingxue
Yu, Yonghao
Exploration of Potential Molecular Targets of Dexmedetomidine in the Intestinal Repair of Burnt Rats
title Exploration of Potential Molecular Targets of Dexmedetomidine in the Intestinal Repair of Burnt Rats
title_full Exploration of Potential Molecular Targets of Dexmedetomidine in the Intestinal Repair of Burnt Rats
title_fullStr Exploration of Potential Molecular Targets of Dexmedetomidine in the Intestinal Repair of Burnt Rats
title_full_unstemmed Exploration of Potential Molecular Targets of Dexmedetomidine in the Intestinal Repair of Burnt Rats
title_short Exploration of Potential Molecular Targets of Dexmedetomidine in the Intestinal Repair of Burnt Rats
title_sort exploration of potential molecular targets of dexmedetomidine in the intestinal repair of burnt rats
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286122/
https://www.ncbi.nlm.nih.gov/pubmed/34285543
http://dx.doi.org/10.2147/JIR.S315952
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