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Angiotensin-converting enzyme 2, the SARS-CoV-2 cellular receptor, is widely expressed in human myometrium and uterine leiomyoma
OBJECTIVE: Angiotensin-converting-enzyme 2 (ACE2), the cell surface receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is found in a variety of reproductive tissues. The present study evaluated whether uterine fibroids and normal myometrium express ACE2 and, if so, at which t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287083/ https://www.ncbi.nlm.nih.gov/pubmed/34307238 http://dx.doi.org/10.1177/2284026520954068 |
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author | Racilan, Alexon M Assis, Wiviane A Casalechi, Maíra Spagnolo-Souza, Ananda Pascoal-Xavier, Marcelo A Simões-e-Silva, Ana C Del Puerto, Helen L Reis, Fernando M |
author_facet | Racilan, Alexon M Assis, Wiviane A Casalechi, Maíra Spagnolo-Souza, Ananda Pascoal-Xavier, Marcelo A Simões-e-Silva, Ana C Del Puerto, Helen L Reis, Fernando M |
author_sort | Racilan, Alexon M |
collection | PubMed |
description | OBJECTIVE: Angiotensin-converting-enzyme 2 (ACE2), the cell surface receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is found in a variety of reproductive tissues. The present study evaluated whether uterine fibroids and normal myometrium express ACE2 and, if so, at which tissue compartments. METHODS: We included 13 premenopausal women (age range 33–50 years, median 40 years) with uterine fibroids undergoing elective hysterectomy or myomectomy. Samples of leiomyoma (n = 12) and normal myometrial tissue (n = 8) were analyzed by immunohistochemistry for protein localization or by real time PCR for mRNA detection. RESULTS: In normal myometrium, ACE2 immunoreactivity was localized in smooth muscle fibers, arteriolar walls, and endothelial cells. In uterine leiomyoma, ACE2 staining was more intense in smooth muscle cells than in the extracellular matrix, and was also present in vascular endothelium. ACE2 mRNA was detected in myometrium as well as in fibroid samples. CONCLUSION: Human myometrium and uterine leiomyoma express ACE2 mRNA and have abundant distribution of ACE2 protein in their smooth muscle cells and microvasculature. |
format | Online Article Text |
id | pubmed-8287083 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-82870832021-07-19 Angiotensin-converting enzyme 2, the SARS-CoV-2 cellular receptor, is widely expressed in human myometrium and uterine leiomyoma Racilan, Alexon M Assis, Wiviane A Casalechi, Maíra Spagnolo-Souza, Ananda Pascoal-Xavier, Marcelo A Simões-e-Silva, Ana C Del Puerto, Helen L Reis, Fernando M J Endometr Pelvic Pain Disord Covid-19 Article OBJECTIVE: Angiotensin-converting-enzyme 2 (ACE2), the cell surface receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is found in a variety of reproductive tissues. The present study evaluated whether uterine fibroids and normal myometrium express ACE2 and, if so, at which tissue compartments. METHODS: We included 13 premenopausal women (age range 33–50 years, median 40 years) with uterine fibroids undergoing elective hysterectomy or myomectomy. Samples of leiomyoma (n = 12) and normal myometrial tissue (n = 8) were analyzed by immunohistochemistry for protein localization or by real time PCR for mRNA detection. RESULTS: In normal myometrium, ACE2 immunoreactivity was localized in smooth muscle fibers, arteriolar walls, and endothelial cells. In uterine leiomyoma, ACE2 staining was more intense in smooth muscle cells than in the extracellular matrix, and was also present in vascular endothelium. ACE2 mRNA was detected in myometrium as well as in fibroid samples. CONCLUSION: Human myometrium and uterine leiomyoma express ACE2 mRNA and have abundant distribution of ACE2 protein in their smooth muscle cells and microvasculature. SAGE Publications 2021-03 /pmc/articles/PMC8287083/ /pubmed/34307238 http://dx.doi.org/10.1177/2284026520954068 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Covid-19 Article Racilan, Alexon M Assis, Wiviane A Casalechi, Maíra Spagnolo-Souza, Ananda Pascoal-Xavier, Marcelo A Simões-e-Silva, Ana C Del Puerto, Helen L Reis, Fernando M Angiotensin-converting enzyme 2, the SARS-CoV-2 cellular receptor, is widely expressed in human myometrium and uterine leiomyoma |
title | Angiotensin-converting enzyme 2, the SARS-CoV-2 cellular receptor, is
widely expressed in human myometrium and uterine leiomyoma |
title_full | Angiotensin-converting enzyme 2, the SARS-CoV-2 cellular receptor, is
widely expressed in human myometrium and uterine leiomyoma |
title_fullStr | Angiotensin-converting enzyme 2, the SARS-CoV-2 cellular receptor, is
widely expressed in human myometrium and uterine leiomyoma |
title_full_unstemmed | Angiotensin-converting enzyme 2, the SARS-CoV-2 cellular receptor, is
widely expressed in human myometrium and uterine leiomyoma |
title_short | Angiotensin-converting enzyme 2, the SARS-CoV-2 cellular receptor, is
widely expressed in human myometrium and uterine leiomyoma |
title_sort | angiotensin-converting enzyme 2, the sars-cov-2 cellular receptor, is
widely expressed in human myometrium and uterine leiomyoma |
topic | Covid-19 Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287083/ https://www.ncbi.nlm.nih.gov/pubmed/34307238 http://dx.doi.org/10.1177/2284026520954068 |
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