Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR

Enzyme encapsulation in metal-organic frameworks (MOFs)/covalent-organic frameworks (COFs) provides advancement in biocatalysis, yet the structural basis underlying the catalytic performance is challenging to probe. Here, we present an effective protocol to determine the orientation and dynamics of...

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Detalles Bibliográficos
Autores principales: Pan, Yanxiong, Li, Hui, Li, Qiaobin, Lenertz, Mary, Schuster, Isabelle, Jordahl, Drew, Zhu, Xiao, Chen, Bingcan, Yang, Zhongyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287244/
https://www.ncbi.nlm.nih.gov/pubmed/34308381
http://dx.doi.org/10.1016/j.xpro.2021.100676
Descripción
Sumario:Enzyme encapsulation in metal-organic frameworks (MOFs)/covalent-organic frameworks (COFs) provides advancement in biocatalysis, yet the structural basis underlying the catalytic performance is challenging to probe. Here, we present an effective protocol to determine the orientation and dynamics of enzymes in MOFs/COFs using site-directed spin labeling and electron paramagnetic resonance spectroscopy. The protocol is demonstrated using lysozyme and can be generalized to other enzymes. For complete information on the generation and use of this protocol, please refer to Pan et al. (2021a).

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