Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR

Enzyme encapsulation in metal-organic frameworks (MOFs)/covalent-organic frameworks (COFs) provides advancement in biocatalysis, yet the structural basis underlying the catalytic performance is challenging to probe. Here, we present an effective protocol to determine the orientation and dynamics of...

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Autores principales: Pan, Yanxiong, Li, Hui, Li, Qiaobin, Lenertz, Mary, Schuster, Isabelle, Jordahl, Drew, Zhu, Xiao, Chen, Bingcan, Yang, Zhongyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287244/
https://www.ncbi.nlm.nih.gov/pubmed/34308381
http://dx.doi.org/10.1016/j.xpro.2021.100676
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author Pan, Yanxiong
Li, Hui
Li, Qiaobin
Lenertz, Mary
Schuster, Isabelle
Jordahl, Drew
Zhu, Xiao
Chen, Bingcan
Yang, Zhongyu
author_facet Pan, Yanxiong
Li, Hui
Li, Qiaobin
Lenertz, Mary
Schuster, Isabelle
Jordahl, Drew
Zhu, Xiao
Chen, Bingcan
Yang, Zhongyu
author_sort Pan, Yanxiong
collection PubMed
description Enzyme encapsulation in metal-organic frameworks (MOFs)/covalent-organic frameworks (COFs) provides advancement in biocatalysis, yet the structural basis underlying the catalytic performance is challenging to probe. Here, we present an effective protocol to determine the orientation and dynamics of enzymes in MOFs/COFs using site-directed spin labeling and electron paramagnetic resonance spectroscopy. The protocol is demonstrated using lysozyme and can be generalized to other enzymes. For complete information on the generation and use of this protocol, please refer to Pan et al. (2021a).
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spelling pubmed-82872442021-07-22 Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR Pan, Yanxiong Li, Hui Li, Qiaobin Lenertz, Mary Schuster, Isabelle Jordahl, Drew Zhu, Xiao Chen, Bingcan Yang, Zhongyu STAR Protoc Protocol Enzyme encapsulation in metal-organic frameworks (MOFs)/covalent-organic frameworks (COFs) provides advancement in biocatalysis, yet the structural basis underlying the catalytic performance is challenging to probe. Here, we present an effective protocol to determine the orientation and dynamics of enzymes in MOFs/COFs using site-directed spin labeling and electron paramagnetic resonance spectroscopy. The protocol is demonstrated using lysozyme and can be generalized to other enzymes. For complete information on the generation and use of this protocol, please refer to Pan et al. (2021a). Elsevier 2021-07-14 /pmc/articles/PMC8287244/ /pubmed/34308381 http://dx.doi.org/10.1016/j.xpro.2021.100676 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Pan, Yanxiong
Li, Hui
Li, Qiaobin
Lenertz, Mary
Schuster, Isabelle
Jordahl, Drew
Zhu, Xiao
Chen, Bingcan
Yang, Zhongyu
Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR
title Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR
title_full Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR
title_fullStr Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR
title_full_unstemmed Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR
title_short Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR
title_sort protocol for resolving enzyme orientation and dynamics in advanced porous materials via sdsl-epr
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287244/
https://www.ncbi.nlm.nih.gov/pubmed/34308381
http://dx.doi.org/10.1016/j.xpro.2021.100676
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