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Herpesviruses Serology Distinguishes Different Subgroups of Patients From the United Kingdom Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Biobank
The evidence of an association between Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and chronic herpesviruses infections remains inconclusive. Two reasons for the lack of consistent evidence are the large heterogeneity of the patients' population with different disease triggers a...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287507/ https://www.ncbi.nlm.nih.gov/pubmed/34291062 http://dx.doi.org/10.3389/fmed.2021.686736 |
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author | Domingues, Tiago Dias Grabowska, Anna D. Lee, Ji-Sook Ameijeiras-Alonso, Jose Westermeier, Francisco Scheibenbogen, Carmen Cliff, Jacqueline M. Nacul, Luis Lacerda, Eliana M. Mouriño, Helena Sepúlveda, Nuno |
author_facet | Domingues, Tiago Dias Grabowska, Anna D. Lee, Ji-Sook Ameijeiras-Alonso, Jose Westermeier, Francisco Scheibenbogen, Carmen Cliff, Jacqueline M. Nacul, Luis Lacerda, Eliana M. Mouriño, Helena Sepúlveda, Nuno |
author_sort | Domingues, Tiago Dias |
collection | PubMed |
description | The evidence of an association between Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and chronic herpesviruses infections remains inconclusive. Two reasons for the lack of consistent evidence are the large heterogeneity of the patients' population with different disease triggers and the use of arbitrary cutoffs for defining seropositivity. In this work we re-analyzed previously published serological data related to 7 herpesvirus antigens. Patients with ME/CFS were subdivided into four subgroups related to the disease triggers: S(0)-42 patients who did not know their disease trigger; S(1)-43 patients who reported a non-infection trigger; S(2)-93 patients who reported an infection trigger, but that infection was not confirmed by a lab test; and S(3)-48 patients who reported an infection trigger and that infection was confirmed by a lab test. In accordance with a sensitivity analysis, the data were compared to those from 99 healthy controls allowing the seropositivity cutoffs to vary within a wide range of possible values. We found a negative association between S(1) and seropositivity to Epstein-Barr virus (VCA and EBNA1 antigens) and Varicella-Zoster virus using specific seropositivity cutoff. However, this association was not significant when controlling for multiple testing. We also found that S(3) had a lower seroprevalence to the human cytomegalovirus when compared to healthy controls for all cutoffs used for seropositivity and after adjusting for multiple testing using the Benjamini-Hochberg procedure. However, this association did not reach statistical significance when using Benjamini-Yekutieli procedure. In summary, herpesviruses serology could distinguish subgroups of ME/CFS patients according to their disease trigger, but this finding could be eventually affected by the problem of multiple testing. |
format | Online Article Text |
id | pubmed-8287507 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82875072021-07-20 Herpesviruses Serology Distinguishes Different Subgroups of Patients From the United Kingdom Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Biobank Domingues, Tiago Dias Grabowska, Anna D. Lee, Ji-Sook Ameijeiras-Alonso, Jose Westermeier, Francisco Scheibenbogen, Carmen Cliff, Jacqueline M. Nacul, Luis Lacerda, Eliana M. Mouriño, Helena Sepúlveda, Nuno Front Med (Lausanne) Medicine The evidence of an association between Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and chronic herpesviruses infections remains inconclusive. Two reasons for the lack of consistent evidence are the large heterogeneity of the patients' population with different disease triggers and the use of arbitrary cutoffs for defining seropositivity. In this work we re-analyzed previously published serological data related to 7 herpesvirus antigens. Patients with ME/CFS were subdivided into four subgroups related to the disease triggers: S(0)-42 patients who did not know their disease trigger; S(1)-43 patients who reported a non-infection trigger; S(2)-93 patients who reported an infection trigger, but that infection was not confirmed by a lab test; and S(3)-48 patients who reported an infection trigger and that infection was confirmed by a lab test. In accordance with a sensitivity analysis, the data were compared to those from 99 healthy controls allowing the seropositivity cutoffs to vary within a wide range of possible values. We found a negative association between S(1) and seropositivity to Epstein-Barr virus (VCA and EBNA1 antigens) and Varicella-Zoster virus using specific seropositivity cutoff. However, this association was not significant when controlling for multiple testing. We also found that S(3) had a lower seroprevalence to the human cytomegalovirus when compared to healthy controls for all cutoffs used for seropositivity and after adjusting for multiple testing using the Benjamini-Hochberg procedure. However, this association did not reach statistical significance when using Benjamini-Yekutieli procedure. In summary, herpesviruses serology could distinguish subgroups of ME/CFS patients according to their disease trigger, but this finding could be eventually affected by the problem of multiple testing. Frontiers Media S.A. 2021-07-05 /pmc/articles/PMC8287507/ /pubmed/34291062 http://dx.doi.org/10.3389/fmed.2021.686736 Text en Copyright © 2021 Domingues, Grabowska, Lee, Ameijeiras-Alonso, Westermeier, Scheibenbogen, Cliff, Nacul, Lacerda, Mouriño and Sepúlveda. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Medicine Domingues, Tiago Dias Grabowska, Anna D. Lee, Ji-Sook Ameijeiras-Alonso, Jose Westermeier, Francisco Scheibenbogen, Carmen Cliff, Jacqueline M. Nacul, Luis Lacerda, Eliana M. Mouriño, Helena Sepúlveda, Nuno Herpesviruses Serology Distinguishes Different Subgroups of Patients From the United Kingdom Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Biobank |
title | Herpesviruses Serology Distinguishes Different Subgroups of Patients From the United Kingdom Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Biobank |
title_full | Herpesviruses Serology Distinguishes Different Subgroups of Patients From the United Kingdom Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Biobank |
title_fullStr | Herpesviruses Serology Distinguishes Different Subgroups of Patients From the United Kingdom Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Biobank |
title_full_unstemmed | Herpesviruses Serology Distinguishes Different Subgroups of Patients From the United Kingdom Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Biobank |
title_short | Herpesviruses Serology Distinguishes Different Subgroups of Patients From the United Kingdom Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Biobank |
title_sort | herpesviruses serology distinguishes different subgroups of patients from the united kingdom myalgic encephalomyelitis/chronic fatigue syndrome biobank |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287507/ https://www.ncbi.nlm.nih.gov/pubmed/34291062 http://dx.doi.org/10.3389/fmed.2021.686736 |
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