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Modifying Effects of Glucose and Insulin/Insulin-Like Growth Factors on Colon Cancer Cells
There are only a few experimental studies which have investigated effects of glucose alone, and glucose in combination with insulin/insulin-like growth factors (IGF) on the growth of colon cancer. In the present study, we studied in vitro in human colorectal cancer cells originating from four Dukes’...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287530/ https://www.ncbi.nlm.nih.gov/pubmed/34290976 http://dx.doi.org/10.3389/fonc.2021.645732 |
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author | Berk, Şeyda Janssen, Joseph A. M. J. L. van Koetsveld, Peter M. Dogan, Fadime Değerli, Naci Özcan, Servet Kelestimur, Fahrettin Hofland, Leo J. |
author_facet | Berk, Şeyda Janssen, Joseph A. M. J. L. van Koetsveld, Peter M. Dogan, Fadime Değerli, Naci Özcan, Servet Kelestimur, Fahrettin Hofland, Leo J. |
author_sort | Berk, Şeyda |
collection | PubMed |
description | There are only a few experimental studies which have investigated effects of glucose alone, and glucose in combination with insulin/insulin-like growth factors (IGF) on the growth of colon cancer. In the present study, we studied in vitro in human colorectal cancer cells originating from four Dukes’ stages of colorectal cancer the effects of glucose, insulin and IGFs on proliferation, migration, cell cycle progression and gene expression of the IGF system. Growth of colon cancer cells originating from a Dukes’ stage A was glucose-dependent, whereas growth of cancer cells from Dukes’ stage B, C and D was glucose-independent. Stimulatory effects of insulin and IGFs on cell growth were observed only in colon cancer cells originating from Dukes’ stage C and D. IGF-II stimulated migration in Dukes’ stage B cells only. The growth stimulatory effects in Dukes’ stage C and D colorectal cancer cells were accompanied by G2/M arrest and associated with an increased IGF-IR/IGF-II receptor ratio. In conclusion, our in vitro data suggest that the stimulating effects of glucose, IGFs and insulin on proliferation differ between colorectal cancer cells from early and late Dukes’ stages. Stimulatory effects of glucose on proliferation appear predominantly present in stage Dukes’ stage A colorectal cancer cells, while in contrast growth factor-mediated stimulation of cell proliferation is more pronounced in Dukes’ late stage (metastasized) colorectal cancer cells. Moreover, our study suggests that a stringent glucose control may be important to control tumor growth in early stages of colorectal cancer, while inhibition of the endocrine actions of the IGFs and insulin become more important in the late (metastasized) stages of colorectal cancer to restrain growth of colon cancer cells. |
format | Online Article Text |
id | pubmed-8287530 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82875302021-07-20 Modifying Effects of Glucose and Insulin/Insulin-Like Growth Factors on Colon Cancer Cells Berk, Şeyda Janssen, Joseph A. M. J. L. van Koetsveld, Peter M. Dogan, Fadime Değerli, Naci Özcan, Servet Kelestimur, Fahrettin Hofland, Leo J. Front Oncol Oncology There are only a few experimental studies which have investigated effects of glucose alone, and glucose in combination with insulin/insulin-like growth factors (IGF) on the growth of colon cancer. In the present study, we studied in vitro in human colorectal cancer cells originating from four Dukes’ stages of colorectal cancer the effects of glucose, insulin and IGFs on proliferation, migration, cell cycle progression and gene expression of the IGF system. Growth of colon cancer cells originating from a Dukes’ stage A was glucose-dependent, whereas growth of cancer cells from Dukes’ stage B, C and D was glucose-independent. Stimulatory effects of insulin and IGFs on cell growth were observed only in colon cancer cells originating from Dukes’ stage C and D. IGF-II stimulated migration in Dukes’ stage B cells only. The growth stimulatory effects in Dukes’ stage C and D colorectal cancer cells were accompanied by G2/M arrest and associated with an increased IGF-IR/IGF-II receptor ratio. In conclusion, our in vitro data suggest that the stimulating effects of glucose, IGFs and insulin on proliferation differ between colorectal cancer cells from early and late Dukes’ stages. Stimulatory effects of glucose on proliferation appear predominantly present in stage Dukes’ stage A colorectal cancer cells, while in contrast growth factor-mediated stimulation of cell proliferation is more pronounced in Dukes’ late stage (metastasized) colorectal cancer cells. Moreover, our study suggests that a stringent glucose control may be important to control tumor growth in early stages of colorectal cancer, while inhibition of the endocrine actions of the IGFs and insulin become more important in the late (metastasized) stages of colorectal cancer to restrain growth of colon cancer cells. Frontiers Media S.A. 2021-07-05 /pmc/articles/PMC8287530/ /pubmed/34290976 http://dx.doi.org/10.3389/fonc.2021.645732 Text en Copyright © 2021 Berk, Janssen, van Koetsveld, Dogan, Değerli, Özcan, Kelestimur and Hofland https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Berk, Şeyda Janssen, Joseph A. M. J. L. van Koetsveld, Peter M. Dogan, Fadime Değerli, Naci Özcan, Servet Kelestimur, Fahrettin Hofland, Leo J. Modifying Effects of Glucose and Insulin/Insulin-Like Growth Factors on Colon Cancer Cells |
title | Modifying Effects of Glucose and Insulin/Insulin-Like Growth Factors on Colon Cancer Cells |
title_full | Modifying Effects of Glucose and Insulin/Insulin-Like Growth Factors on Colon Cancer Cells |
title_fullStr | Modifying Effects of Glucose and Insulin/Insulin-Like Growth Factors on Colon Cancer Cells |
title_full_unstemmed | Modifying Effects of Glucose and Insulin/Insulin-Like Growth Factors on Colon Cancer Cells |
title_short | Modifying Effects of Glucose and Insulin/Insulin-Like Growth Factors on Colon Cancer Cells |
title_sort | modifying effects of glucose and insulin/insulin-like growth factors on colon cancer cells |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287530/ https://www.ncbi.nlm.nih.gov/pubmed/34290976 http://dx.doi.org/10.3389/fonc.2021.645732 |
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