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Modifying Effects of Glucose and Insulin/Insulin-Like Growth Factors on Colon Cancer Cells

There are only a few experimental studies which have investigated effects of glucose alone, and glucose in combination with insulin/insulin-like growth factors (IGF) on the growth of colon cancer. In the present study, we studied in vitro in human colorectal cancer cells originating from four Dukes’...

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Autores principales: Berk, Şeyda, Janssen, Joseph A. M. J. L., van Koetsveld, Peter M., Dogan, Fadime, Değerli, Naci, Özcan, Servet, Kelestimur, Fahrettin, Hofland, Leo J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287530/
https://www.ncbi.nlm.nih.gov/pubmed/34290976
http://dx.doi.org/10.3389/fonc.2021.645732
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author Berk, Şeyda
Janssen, Joseph A. M. J. L.
van Koetsveld, Peter M.
Dogan, Fadime
Değerli, Naci
Özcan, Servet
Kelestimur, Fahrettin
Hofland, Leo J.
author_facet Berk, Şeyda
Janssen, Joseph A. M. J. L.
van Koetsveld, Peter M.
Dogan, Fadime
Değerli, Naci
Özcan, Servet
Kelestimur, Fahrettin
Hofland, Leo J.
author_sort Berk, Şeyda
collection PubMed
description There are only a few experimental studies which have investigated effects of glucose alone, and glucose in combination with insulin/insulin-like growth factors (IGF) on the growth of colon cancer. In the present study, we studied in vitro in human colorectal cancer cells originating from four Dukes’ stages of colorectal cancer the effects of glucose, insulin and IGFs on proliferation, migration, cell cycle progression and gene expression of the IGF system. Growth of colon cancer cells originating from a Dukes’ stage A was glucose-dependent, whereas growth of cancer cells from Dukes’ stage B, C and D was glucose-independent. Stimulatory effects of insulin and IGFs on cell growth were observed only in colon cancer cells originating from Dukes’ stage C and D. IGF-II stimulated migration in Dukes’ stage B cells only. The growth stimulatory effects in Dukes’ stage C and D colorectal cancer cells were accompanied by G2/M arrest and associated with an increased IGF-IR/IGF-II receptor ratio. In conclusion, our in vitro data suggest that the stimulating effects of glucose, IGFs and insulin on proliferation differ between colorectal cancer cells from early and late Dukes’ stages. Stimulatory effects of glucose on proliferation appear predominantly present in stage Dukes’ stage A colorectal cancer cells, while in contrast growth factor-mediated stimulation of cell proliferation is more pronounced in Dukes’ late stage (metastasized) colorectal cancer cells. Moreover, our study suggests that a stringent glucose control may be important to control tumor growth in early stages of colorectal cancer, while inhibition of the endocrine actions of the IGFs and insulin become more important in the late (metastasized) stages of colorectal cancer to restrain growth of colon cancer cells.
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spelling pubmed-82875302021-07-20 Modifying Effects of Glucose and Insulin/Insulin-Like Growth Factors on Colon Cancer Cells Berk, Şeyda Janssen, Joseph A. M. J. L. van Koetsveld, Peter M. Dogan, Fadime Değerli, Naci Özcan, Servet Kelestimur, Fahrettin Hofland, Leo J. Front Oncol Oncology There are only a few experimental studies which have investigated effects of glucose alone, and glucose in combination with insulin/insulin-like growth factors (IGF) on the growth of colon cancer. In the present study, we studied in vitro in human colorectal cancer cells originating from four Dukes’ stages of colorectal cancer the effects of glucose, insulin and IGFs on proliferation, migration, cell cycle progression and gene expression of the IGF system. Growth of colon cancer cells originating from a Dukes’ stage A was glucose-dependent, whereas growth of cancer cells from Dukes’ stage B, C and D was glucose-independent. Stimulatory effects of insulin and IGFs on cell growth were observed only in colon cancer cells originating from Dukes’ stage C and D. IGF-II stimulated migration in Dukes’ stage B cells only. The growth stimulatory effects in Dukes’ stage C and D colorectal cancer cells were accompanied by G2/M arrest and associated with an increased IGF-IR/IGF-II receptor ratio. In conclusion, our in vitro data suggest that the stimulating effects of glucose, IGFs and insulin on proliferation differ between colorectal cancer cells from early and late Dukes’ stages. Stimulatory effects of glucose on proliferation appear predominantly present in stage Dukes’ stage A colorectal cancer cells, while in contrast growth factor-mediated stimulation of cell proliferation is more pronounced in Dukes’ late stage (metastasized) colorectal cancer cells. Moreover, our study suggests that a stringent glucose control may be important to control tumor growth in early stages of colorectal cancer, while inhibition of the endocrine actions of the IGFs and insulin become more important in the late (metastasized) stages of colorectal cancer to restrain growth of colon cancer cells. Frontiers Media S.A. 2021-07-05 /pmc/articles/PMC8287530/ /pubmed/34290976 http://dx.doi.org/10.3389/fonc.2021.645732 Text en Copyright © 2021 Berk, Janssen, van Koetsveld, Dogan, Değerli, Özcan, Kelestimur and Hofland https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Berk, Şeyda
Janssen, Joseph A. M. J. L.
van Koetsveld, Peter M.
Dogan, Fadime
Değerli, Naci
Özcan, Servet
Kelestimur, Fahrettin
Hofland, Leo J.
Modifying Effects of Glucose and Insulin/Insulin-Like Growth Factors on Colon Cancer Cells
title Modifying Effects of Glucose and Insulin/Insulin-Like Growth Factors on Colon Cancer Cells
title_full Modifying Effects of Glucose and Insulin/Insulin-Like Growth Factors on Colon Cancer Cells
title_fullStr Modifying Effects of Glucose and Insulin/Insulin-Like Growth Factors on Colon Cancer Cells
title_full_unstemmed Modifying Effects of Glucose and Insulin/Insulin-Like Growth Factors on Colon Cancer Cells
title_short Modifying Effects of Glucose and Insulin/Insulin-Like Growth Factors on Colon Cancer Cells
title_sort modifying effects of glucose and insulin/insulin-like growth factors on colon cancer cells
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287530/
https://www.ncbi.nlm.nih.gov/pubmed/34290976
http://dx.doi.org/10.3389/fonc.2021.645732
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