Cargando…

A Sequential Three-Phase Pathway Constitutes Tracheary Element Connection in the Arabidopsis/Nicotiana Interfamilial Grafts

Scion-rootstock union formation is a critical step toward the functional assemblage of heterogeneous plants. Interfamilial scion-rootstock interaction often results in graft incompatibility during the assemblage process, and the underlying mechanisms are largely unknown. In this study, we reported t...

Descripción completa

Detalles Bibliográficos
Autores principales: Deng, Zhuying, Wu, Huiyan, Jin, Tianlin, Cai, Tingting, Jiang, Mengting, Wang, Mi, Liang, Dacheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287886/
https://www.ncbi.nlm.nih.gov/pubmed/34290723
http://dx.doi.org/10.3389/fpls.2021.664342
_version_ 1783723996340027392
author Deng, Zhuying
Wu, Huiyan
Jin, Tianlin
Cai, Tingting
Jiang, Mengting
Wang, Mi
Liang, Dacheng
author_facet Deng, Zhuying
Wu, Huiyan
Jin, Tianlin
Cai, Tingting
Jiang, Mengting
Wang, Mi
Liang, Dacheng
author_sort Deng, Zhuying
collection PubMed
description Scion-rootstock union formation is a critical step toward the functional assemblage of heterogeneous plants. Interfamilial scion-rootstock interaction often results in graft incompatibility during the assemblage process, and the underlying mechanisms are largely unknown. In this study, we reported that tracheary element (TE) remodeling, including TE segmentation and deformation, rather than de novo formation from callus or adjacent tissues, took place at the early stage of grafting interface between Arabidopsis thaliana and Nicotiana benthamiana (At/Nb). Following cellular deposits, the short TEs from both partners were overlapping, dependent on the homogeneity of contacting TEs, with each other. Without overlapping, the TEs at the interface would grow laterally, and the TEs above and below the interface would undergo self-fusion to form insulating spiraling bundles. Finally, the overlapping TEs constituted a continuous network through alignment. Our results provide a definitive framework for the critical process of TE behavior in the At/Nb distant grafts, including (1) segmentation and/or deformation, (2) matching, overlapping, and cellular deposits, and (3) aligning or spiraling. These insights might guide us in the future into constructing more compatible distant grafts from the perspective of TE homogeneity.
format Online
Article
Text
id pubmed-8287886
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-82878862021-07-20 A Sequential Three-Phase Pathway Constitutes Tracheary Element Connection in the Arabidopsis/Nicotiana Interfamilial Grafts Deng, Zhuying Wu, Huiyan Jin, Tianlin Cai, Tingting Jiang, Mengting Wang, Mi Liang, Dacheng Front Plant Sci Plant Science Scion-rootstock union formation is a critical step toward the functional assemblage of heterogeneous plants. Interfamilial scion-rootstock interaction often results in graft incompatibility during the assemblage process, and the underlying mechanisms are largely unknown. In this study, we reported that tracheary element (TE) remodeling, including TE segmentation and deformation, rather than de novo formation from callus or adjacent tissues, took place at the early stage of grafting interface between Arabidopsis thaliana and Nicotiana benthamiana (At/Nb). Following cellular deposits, the short TEs from both partners were overlapping, dependent on the homogeneity of contacting TEs, with each other. Without overlapping, the TEs at the interface would grow laterally, and the TEs above and below the interface would undergo self-fusion to form insulating spiraling bundles. Finally, the overlapping TEs constituted a continuous network through alignment. Our results provide a definitive framework for the critical process of TE behavior in the At/Nb distant grafts, including (1) segmentation and/or deformation, (2) matching, overlapping, and cellular deposits, and (3) aligning or spiraling. These insights might guide us in the future into constructing more compatible distant grafts from the perspective of TE homogeneity. Frontiers Media S.A. 2021-07-05 /pmc/articles/PMC8287886/ /pubmed/34290723 http://dx.doi.org/10.3389/fpls.2021.664342 Text en Copyright © 2021 Deng, Wu, Jin, Cai, Jiang, Wang and Liang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Deng, Zhuying
Wu, Huiyan
Jin, Tianlin
Cai, Tingting
Jiang, Mengting
Wang, Mi
Liang, Dacheng
A Sequential Three-Phase Pathway Constitutes Tracheary Element Connection in the Arabidopsis/Nicotiana Interfamilial Grafts
title A Sequential Three-Phase Pathway Constitutes Tracheary Element Connection in the Arabidopsis/Nicotiana Interfamilial Grafts
title_full A Sequential Three-Phase Pathway Constitutes Tracheary Element Connection in the Arabidopsis/Nicotiana Interfamilial Grafts
title_fullStr A Sequential Three-Phase Pathway Constitutes Tracheary Element Connection in the Arabidopsis/Nicotiana Interfamilial Grafts
title_full_unstemmed A Sequential Three-Phase Pathway Constitutes Tracheary Element Connection in the Arabidopsis/Nicotiana Interfamilial Grafts
title_short A Sequential Three-Phase Pathway Constitutes Tracheary Element Connection in the Arabidopsis/Nicotiana Interfamilial Grafts
title_sort sequential three-phase pathway constitutes tracheary element connection in the arabidopsis/nicotiana interfamilial grafts
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287886/
https://www.ncbi.nlm.nih.gov/pubmed/34290723
http://dx.doi.org/10.3389/fpls.2021.664342
work_keys_str_mv AT dengzhuying asequentialthreephasepathwayconstitutestrachearyelementconnectioninthearabidopsisnicotianainterfamilialgrafts
AT wuhuiyan asequentialthreephasepathwayconstitutestrachearyelementconnectioninthearabidopsisnicotianainterfamilialgrafts
AT jintianlin asequentialthreephasepathwayconstitutestrachearyelementconnectioninthearabidopsisnicotianainterfamilialgrafts
AT caitingting asequentialthreephasepathwayconstitutestrachearyelementconnectioninthearabidopsisnicotianainterfamilialgrafts
AT jiangmengting asequentialthreephasepathwayconstitutestrachearyelementconnectioninthearabidopsisnicotianainterfamilialgrafts
AT wangmi asequentialthreephasepathwayconstitutestrachearyelementconnectioninthearabidopsisnicotianainterfamilialgrafts
AT liangdacheng asequentialthreephasepathwayconstitutestrachearyelementconnectioninthearabidopsisnicotianainterfamilialgrafts
AT dengzhuying sequentialthreephasepathwayconstitutestrachearyelementconnectioninthearabidopsisnicotianainterfamilialgrafts
AT wuhuiyan sequentialthreephasepathwayconstitutestrachearyelementconnectioninthearabidopsisnicotianainterfamilialgrafts
AT jintianlin sequentialthreephasepathwayconstitutestrachearyelementconnectioninthearabidopsisnicotianainterfamilialgrafts
AT caitingting sequentialthreephasepathwayconstitutestrachearyelementconnectioninthearabidopsisnicotianainterfamilialgrafts
AT jiangmengting sequentialthreephasepathwayconstitutestrachearyelementconnectioninthearabidopsisnicotianainterfamilialgrafts
AT wangmi sequentialthreephasepathwayconstitutestrachearyelementconnectioninthearabidopsisnicotianainterfamilialgrafts
AT liangdacheng sequentialthreephasepathwayconstitutestrachearyelementconnectioninthearabidopsisnicotianainterfamilialgrafts