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Negative Impact of Citral on Susceptibility of Pseudomonas aeruginosa to Antibiotics

Essential oils (EOs) or their components are widely used by inhalation or nebulization to fight mild respiratory bacterial infections. However, their interaction with antibiotics is poorly known. In this study we evaluated the effects of citral, the main component of lemongrass oil, on in vitro susc...

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Autores principales: Tetard, Alexandre, Foley, Sarah, Mislin, Gaëtan L. A., Brunel, Jean-Michel, Oliva, Estefania, Torrealba Anzola, Freddy, Zedet, Andy, Cardey, Bruno, Pellequer, Yann, Ramseyer, Christophe, Plésiat, Patrick, Llanes, Catherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287888/
https://www.ncbi.nlm.nih.gov/pubmed/34290691
http://dx.doi.org/10.3389/fmicb.2021.709838
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author Tetard, Alexandre
Foley, Sarah
Mislin, Gaëtan L. A.
Brunel, Jean-Michel
Oliva, Estefania
Torrealba Anzola, Freddy
Zedet, Andy
Cardey, Bruno
Pellequer, Yann
Ramseyer, Christophe
Plésiat, Patrick
Llanes, Catherine
author_facet Tetard, Alexandre
Foley, Sarah
Mislin, Gaëtan L. A.
Brunel, Jean-Michel
Oliva, Estefania
Torrealba Anzola, Freddy
Zedet, Andy
Cardey, Bruno
Pellequer, Yann
Ramseyer, Christophe
Plésiat, Patrick
Llanes, Catherine
author_sort Tetard, Alexandre
collection PubMed
description Essential oils (EOs) or their components are widely used by inhalation or nebulization to fight mild respiratory bacterial infections. However, their interaction with antibiotics is poorly known. In this study we evaluated the effects of citral, the main component of lemongrass oil, on in vitro susceptibility of Pseudomonas aeruginosa to antibiotics. Exposure of strain PA14 to subinhibitory concentrations of citral increased expression of operons encoding the multidrug efflux systems MexEF-OprN and MexXY/OprM, and bacterial resistance to anti-pseudomonal antibiotics including imipenem (twofold), gentamicin (eightfold), tobramycin (eightfold), ciprofloxacin (twofold), and colistin (≥128-fold). Use of pump deletion mutants showed that in addition to efflux other mechanisms were involved in this citral-induced phenotype. Determination of Zeta potential suggested that citral impairs the cell surface binding of aminoglycosides and colistin used at low concentrations (≤10 μg/mL). Moreover, experiments based on Raman spectroscopy and high-resolution mass spectrometry demonstrated formation of a Schiff base between the aldehyde group of citral and amino-groups of tobramycin and colistin. Chemical synthesis of tobracitryl, the imine compound resulting from condensation of citral and tobramycin, confirmed the loss of antibiotic activity due to adduct formation. Altogether these data point to the potential risk concern of self-medication with EOs containing citral in patients suffering from P. aeruginosa chronic lung infections and being treated with aerosols of aminoglycoside or colistin.
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spelling pubmed-82878882021-07-20 Negative Impact of Citral on Susceptibility of Pseudomonas aeruginosa to Antibiotics Tetard, Alexandre Foley, Sarah Mislin, Gaëtan L. A. Brunel, Jean-Michel Oliva, Estefania Torrealba Anzola, Freddy Zedet, Andy Cardey, Bruno Pellequer, Yann Ramseyer, Christophe Plésiat, Patrick Llanes, Catherine Front Microbiol Microbiology Essential oils (EOs) or their components are widely used by inhalation or nebulization to fight mild respiratory bacterial infections. However, their interaction with antibiotics is poorly known. In this study we evaluated the effects of citral, the main component of lemongrass oil, on in vitro susceptibility of Pseudomonas aeruginosa to antibiotics. Exposure of strain PA14 to subinhibitory concentrations of citral increased expression of operons encoding the multidrug efflux systems MexEF-OprN and MexXY/OprM, and bacterial resistance to anti-pseudomonal antibiotics including imipenem (twofold), gentamicin (eightfold), tobramycin (eightfold), ciprofloxacin (twofold), and colistin (≥128-fold). Use of pump deletion mutants showed that in addition to efflux other mechanisms were involved in this citral-induced phenotype. Determination of Zeta potential suggested that citral impairs the cell surface binding of aminoglycosides and colistin used at low concentrations (≤10 μg/mL). Moreover, experiments based on Raman spectroscopy and high-resolution mass spectrometry demonstrated formation of a Schiff base between the aldehyde group of citral and amino-groups of tobramycin and colistin. Chemical synthesis of tobracitryl, the imine compound resulting from condensation of citral and tobramycin, confirmed the loss of antibiotic activity due to adduct formation. Altogether these data point to the potential risk concern of self-medication with EOs containing citral in patients suffering from P. aeruginosa chronic lung infections and being treated with aerosols of aminoglycoside or colistin. Frontiers Media S.A. 2021-07-05 /pmc/articles/PMC8287888/ /pubmed/34290691 http://dx.doi.org/10.3389/fmicb.2021.709838 Text en Copyright © 2021 Tetard, Foley, Mislin, Brunel, Oliva, Torrealba Anzola, Zedet, Cardey, Pellequer, Ramseyer, Plésiat and Llanes. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Tetard, Alexandre
Foley, Sarah
Mislin, Gaëtan L. A.
Brunel, Jean-Michel
Oliva, Estefania
Torrealba Anzola, Freddy
Zedet, Andy
Cardey, Bruno
Pellequer, Yann
Ramseyer, Christophe
Plésiat, Patrick
Llanes, Catherine
Negative Impact of Citral on Susceptibility of Pseudomonas aeruginosa to Antibiotics
title Negative Impact of Citral on Susceptibility of Pseudomonas aeruginosa to Antibiotics
title_full Negative Impact of Citral on Susceptibility of Pseudomonas aeruginosa to Antibiotics
title_fullStr Negative Impact of Citral on Susceptibility of Pseudomonas aeruginosa to Antibiotics
title_full_unstemmed Negative Impact of Citral on Susceptibility of Pseudomonas aeruginosa to Antibiotics
title_short Negative Impact of Citral on Susceptibility of Pseudomonas aeruginosa to Antibiotics
title_sort negative impact of citral on susceptibility of pseudomonas aeruginosa to antibiotics
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287888/
https://www.ncbi.nlm.nih.gov/pubmed/34290691
http://dx.doi.org/10.3389/fmicb.2021.709838
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