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Negative Impact of Citral on Susceptibility of Pseudomonas aeruginosa to Antibiotics
Essential oils (EOs) or their components are widely used by inhalation or nebulization to fight mild respiratory bacterial infections. However, their interaction with antibiotics is poorly known. In this study we evaluated the effects of citral, the main component of lemongrass oil, on in vitro susc...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287888/ https://www.ncbi.nlm.nih.gov/pubmed/34290691 http://dx.doi.org/10.3389/fmicb.2021.709838 |
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author | Tetard, Alexandre Foley, Sarah Mislin, Gaëtan L. A. Brunel, Jean-Michel Oliva, Estefania Torrealba Anzola, Freddy Zedet, Andy Cardey, Bruno Pellequer, Yann Ramseyer, Christophe Plésiat, Patrick Llanes, Catherine |
author_facet | Tetard, Alexandre Foley, Sarah Mislin, Gaëtan L. A. Brunel, Jean-Michel Oliva, Estefania Torrealba Anzola, Freddy Zedet, Andy Cardey, Bruno Pellequer, Yann Ramseyer, Christophe Plésiat, Patrick Llanes, Catherine |
author_sort | Tetard, Alexandre |
collection | PubMed |
description | Essential oils (EOs) or their components are widely used by inhalation or nebulization to fight mild respiratory bacterial infections. However, their interaction with antibiotics is poorly known. In this study we evaluated the effects of citral, the main component of lemongrass oil, on in vitro susceptibility of Pseudomonas aeruginosa to antibiotics. Exposure of strain PA14 to subinhibitory concentrations of citral increased expression of operons encoding the multidrug efflux systems MexEF-OprN and MexXY/OprM, and bacterial resistance to anti-pseudomonal antibiotics including imipenem (twofold), gentamicin (eightfold), tobramycin (eightfold), ciprofloxacin (twofold), and colistin (≥128-fold). Use of pump deletion mutants showed that in addition to efflux other mechanisms were involved in this citral-induced phenotype. Determination of Zeta potential suggested that citral impairs the cell surface binding of aminoglycosides and colistin used at low concentrations (≤10 μg/mL). Moreover, experiments based on Raman spectroscopy and high-resolution mass spectrometry demonstrated formation of a Schiff base between the aldehyde group of citral and amino-groups of tobramycin and colistin. Chemical synthesis of tobracitryl, the imine compound resulting from condensation of citral and tobramycin, confirmed the loss of antibiotic activity due to adduct formation. Altogether these data point to the potential risk concern of self-medication with EOs containing citral in patients suffering from P. aeruginosa chronic lung infections and being treated with aerosols of aminoglycoside or colistin. |
format | Online Article Text |
id | pubmed-8287888 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82878882021-07-20 Negative Impact of Citral on Susceptibility of Pseudomonas aeruginosa to Antibiotics Tetard, Alexandre Foley, Sarah Mislin, Gaëtan L. A. Brunel, Jean-Michel Oliva, Estefania Torrealba Anzola, Freddy Zedet, Andy Cardey, Bruno Pellequer, Yann Ramseyer, Christophe Plésiat, Patrick Llanes, Catherine Front Microbiol Microbiology Essential oils (EOs) or their components are widely used by inhalation or nebulization to fight mild respiratory bacterial infections. However, their interaction with antibiotics is poorly known. In this study we evaluated the effects of citral, the main component of lemongrass oil, on in vitro susceptibility of Pseudomonas aeruginosa to antibiotics. Exposure of strain PA14 to subinhibitory concentrations of citral increased expression of operons encoding the multidrug efflux systems MexEF-OprN and MexXY/OprM, and bacterial resistance to anti-pseudomonal antibiotics including imipenem (twofold), gentamicin (eightfold), tobramycin (eightfold), ciprofloxacin (twofold), and colistin (≥128-fold). Use of pump deletion mutants showed that in addition to efflux other mechanisms were involved in this citral-induced phenotype. Determination of Zeta potential suggested that citral impairs the cell surface binding of aminoglycosides and colistin used at low concentrations (≤10 μg/mL). Moreover, experiments based on Raman spectroscopy and high-resolution mass spectrometry demonstrated formation of a Schiff base between the aldehyde group of citral and amino-groups of tobramycin and colistin. Chemical synthesis of tobracitryl, the imine compound resulting from condensation of citral and tobramycin, confirmed the loss of antibiotic activity due to adduct formation. Altogether these data point to the potential risk concern of self-medication with EOs containing citral in patients suffering from P. aeruginosa chronic lung infections and being treated with aerosols of aminoglycoside or colistin. Frontiers Media S.A. 2021-07-05 /pmc/articles/PMC8287888/ /pubmed/34290691 http://dx.doi.org/10.3389/fmicb.2021.709838 Text en Copyright © 2021 Tetard, Foley, Mislin, Brunel, Oliva, Torrealba Anzola, Zedet, Cardey, Pellequer, Ramseyer, Plésiat and Llanes. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Tetard, Alexandre Foley, Sarah Mislin, Gaëtan L. A. Brunel, Jean-Michel Oliva, Estefania Torrealba Anzola, Freddy Zedet, Andy Cardey, Bruno Pellequer, Yann Ramseyer, Christophe Plésiat, Patrick Llanes, Catherine Negative Impact of Citral on Susceptibility of Pseudomonas aeruginosa to Antibiotics |
title | Negative Impact of Citral on Susceptibility of Pseudomonas aeruginosa to Antibiotics |
title_full | Negative Impact of Citral on Susceptibility of Pseudomonas aeruginosa to Antibiotics |
title_fullStr | Negative Impact of Citral on Susceptibility of Pseudomonas aeruginosa to Antibiotics |
title_full_unstemmed | Negative Impact of Citral on Susceptibility of Pseudomonas aeruginosa to Antibiotics |
title_short | Negative Impact of Citral on Susceptibility of Pseudomonas aeruginosa to Antibiotics |
title_sort | negative impact of citral on susceptibility of pseudomonas aeruginosa to antibiotics |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287888/ https://www.ncbi.nlm.nih.gov/pubmed/34290691 http://dx.doi.org/10.3389/fmicb.2021.709838 |
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