Efficient multiplexed gene regulation in Saccharomyces cerevisiae using dCas12a

CRISPR Cas12a is an RNA-programmable endonuclease particularly suitable for gene regulation. This is due to its preference for T-rich PAMs that allows it to more easily target AT-rich promoter sequences, and built-in RNase activity which can process a single CRISPR RNA array encoding multiple spacer...

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Detalles Bibliográficos
Autores principales: Ciurkot, Klaudia, Gorochowski, Thomas E, Roubos, Johannes A, Verwaal, René
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287914/
https://www.ncbi.nlm.nih.gov/pubmed/34197613
http://dx.doi.org/10.1093/nar/gkab529

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287914/
https://www.ncbi.nlm.nih.gov/pubmed/34197613
http://dx.doi.org/10.1093/nar/gkab529

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